RL2792 (R. leguminosarum bv. viciae 3841 )
In pET16-DEST (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  RL2792
Gene Name:  beta-mannosidase
Original Clone ID:
BGH02_B02c
Keyword:
full-length CDS
Species:
Rhizobium leguminosarum bv. viciae 3841
Type:
cDNA
Vector Name:
pET16-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term 6xHis tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
RlCD00405581
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 2460nts
Open reading frame: 1 to 2460

ACATCCTTGCCGAAGAACGGTTTCAACATCGACCTTTCCGGTTCGTGGCAGCTCGCCTCC
GTCGACGGCGAGATCCGCACCGCGATCATGCTGCCGGGCGACGTGCACACGGCTCTGCAC
CGGGCAGGATTGATCCCCGATCCCTATTTCGGCCGCAACGAGGAAAAGGTGCAGTGGGTC
GCCGAGCGCGAATGGGCGGTCGAGCGCAGCTTCGCGCTTCAGGAGGTCGAGGGCGACTGG
TATCTCGATATCGATTATCTCGACACGGTTGCCAGCGTCCACGTCAACGGCTTTCTGGCG
CTCGAAGCCGACAACAGCTTCCGCCGCTACCGGCCCGATGTCTCCAGCATGCTGAAATCA
GGCGATAACAGCATCCGCATCGTCTTTGCCTCCAACATCGCTGTCGGTGCCGCGCGACAG
AAGCAGCAGCCCTTCTACATTCCCTACAGCACGGGCAACTCACCGATCCCCGACGGCAAC
ATGCTGCGCAAGCCGCAATGCCATTTCGGCTGGGACTGGAACATCGCCATTGCCCCGCTC
GGTCTTTACGGAACGATCGCGCTGAAAAAGCTCGAAACGGCGCGCATCGAACACGTCGTC
ACCCGCCAGACCCACAATAATGACGGTTCGGTCGACCTCAAAGTGACGGCGACGCTGTTT
TCCAAGGGGCCAGACATTGCCCAGGTCTATTTCGACCTCGATGGCGAGCGCGTGCGCCTG
GATGTCGGCGTCAATGGCGAGACCCATGTCAACCATCTCTTCCATATCGACAATCCGCGC
CTCTGGTGGCCCTCCGGCAGCGGCGAGCAGGCGCTCTACAGGCTTTCCGTCGAATTGCCG
ACGGATGAGGTGACCAAGCAGATCGGTCTTCGCACCATCGAGCTGATCACCACGCCGGAT
GCGGCCGGCAGCCGCTTTGCCTTCAAGGTCAACGGCCGCGAGATCTTTTGCCGCGGCGCC
AACTGGATCCCGGCCGACGCGCTGTTTTCGCTGTCTTCACCTGAGAAGACCGAAGACCTG
CTGCAATCGGCCAAAGCCGCCAACATGAACATGATCCGTGTCTGGGGCGGCGGCTTCTAT
GAGCAGGATCATTTCTACGATCTCTGCGACCGGCTGGGCCTTATGGTCTGGCAGGACTTC
ATGTTTGCCTGCAACCTCTATCCGTCGACCGAGGACTTTCTCGACAATGTGACGATCGAG
GTCGACTACCAGGTGCGCCGGCTCTCCTCGCATGCCTCGATCGTGCTCTGGTGCGGCGAC
AATGAACTGGTCGGAGCGCTCACCTGGTTCGAGGAATCGCGGAAGGATCGCGACCGCTAT
CTCGTCTCTTATGATCGCCTCAACCGCACGATCGAGCAGGCAGTGAAAAAGGCGCTGCCC
GGCGCGGTCTGGTGGCCGTCGAGCCCGGCCTCCGGCTATCTCGATTTCGGCGATGCCTGG
CATGCGGACGGGTCCGGCGACATGCATTACTGGTCGGTCTGGCACGAGAACAAGTCGTTC
GACAATTACCGCTCGGTGCGTCCGCGCTTCTGCTCGGAATTCGGCTTCCAGTCCTATACG
TCGCTGCCTGTGATCAAGACCTATGCCGAGGCGAAAGACATGAACGTCGCTTCCCCGGTC
ATGGAGCTGCACCAGAAGAATGCCGGCGGCAACGAGCGCATCGCCGGCACGATGTTCCGT
TATTTCCGATTCCCCAAGGATTTCCCGAACTTCGTCTATCTCTCCCAGATCCAACAGGGG
CTGGCGATCAAGACGGCGGTGGAATACTGGCGGTCGCTCAAACCCCATTGCATGGGCACG
ATCTACTGGCAGCTCAACGACACCTGGCCGGTCGCCTCCTGGTCGAGCCTCGACTATGGC
GGCCGCTGGAAGGCGATGCACTATCTCGTCAAGCGCTTCTTCCAGCCGGTCGCGGTCGCG
GCCATTCCTTCCGACGACGGAAAGACGATTCGCTTCTCGCTGGTCAACGACACGCTTGCC
GATGTCAGCGTCGACCTTTCGATCTCGCTGCTGACGATGCAGGGCGAACGCCGGCACCTG
AGAGACGTACAGGCCATATGTTCGCCGGATGCGGCCGTCACTGCCACGAGCATCGACGTT
TCCGATATTCCCGAGGGAACGTTGCTTGCCTGGCGTTTCACCGCGTCGAACGGCACGGGC
GGCGAGGGGCACCATGTCAACGGTACCTACAAGGCGCTGGAGCTGGAACCCGCGGGGCTG
ACAGTCACCCACGAATATGTCGAGGAGAATGGCTCGGTCGACATCAACGTCACCGCGAAG
GGACTTGCGCTTTTCGTGATGATCGAAACCGAGACCGACGGCAAATATTCCGACAACGCC
TTCGATCTTGCGGCCGGCGAGAGCCGCCGCATCGCCTTTACCCCGGCACGGCCGCTCGAT
CGTGGCGCGCTTCCGGAGTTCCGGTTCTACGACCTGTATTCCTGCCAGTCGGCGGATTAG

Protein sequence predicted based on nucleotide sequence

TSLPKNGFNIDLSGSWQLASVDGEIRTAIMLPGDVHTALHRAGLIPDPYFGRNEEKVQWV
AEREWAVERSFALQEVEGDWYLDIDYLDTVASVHVNGFLALEADNSFRRYRPDVSSMLKS
GDNSIRIVFASNIAVGAARQKQQPFYIPYSTGNSPIPDGNMLRKPQCHFGWDWNIAIAPL
GLYGTIALKKLETARIEHVVTRQTHNNDGSVDLKVTATLFSKGPDIAQVYFDLDGERVRL
DVGVNGETHVNHLFHIDNPRLWWPSGSGEQALYRLSVELPTDEVTKQIGLRTIELITTPD
AAGSRFAFKVNGREIFCRGANWIPADALFSLSSPEKTEDLLQSAKAANMNMIRVWGGGFY
EQDHFYDLCDRLGLMVWQDFMFACNLYPSTEDFLDNVTIEVDYQVRRLSSHASIVLWCGD
NELVGALTWFEESRKDRDRYLVSYDRLNRTIEQAVKKALPGAVWWPSSPASGYLDFGDAW
HADGSGDMHYWSVWHENKSFDNYRSVRPRFCSEFGFQSYTSLPVIKTYAEAKDMNVASPV
MELHQKNAGGNERIAGTMFRYFRFPKDFPNFVYLSQIQQGLAIKTAVEYWRSLKPHCMGT
IYWQLNDTWPVASWSSLDYGGRWKAMHYLVKRFFQPVAVAAIPSDDGKTIRFSLVNDTLA
DVSVDLSISLLTMQGERRHLRDVQAICSPDAAVTATSIDVSDIPEGTLLAWRFTASNGTG
GEGHHVNGTYKALELEPAGLTVTHEYVEENGSVDINVTAKGLALFVMIETETDGKYSDNA
FDLAAGESRRIAFTPARPLDRGALPEFRFYDLYSCQSAD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable