BT_0458 (B. thetaiotaomicron VPI-5482 )
In pET16-DEST (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_0458
Gene Name:  beta-mannosidase
Original Clone ID:
BGH02_B01a
Keyword:
partial CDS
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pET16-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
N-term 6xHis tag and TEV cleavage site
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
BtCD00405592
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 2520nts
Open reading frame: 1 to 2520

CAAGGTAATGACACTTCGGAAGTCATGCTACTGGATACCGGTTGGGAATTTTCTCAATCA
GGGACTGAAAAGTGGATGCCAGCCACGGTTCCTGGTACGGTTCATCAGGACCTGATCAGT
CATGAATTACTGCCGAATCCTTTTTATGGGATGAATGAAAAGAAAATTCAATGGGTGGAG
AATGAGGACTGGGAATATAGAACCTCCTTTATAGTCTCCGAAGAGCAATTGAATCGTGAC
GGAATCCAACTTATTTTTGAAGGTCTTGATACCTATGCAGACGTATATCTCAACGGCTCA
TTACTGCTGAAGGCTGATAATATGTTTGTGGGTTATACACTTCCTGTAAAATCTGTGTTG
CGGAAGGGCGAGAATCACTTATATATTTATTTCCATTCACCTATTCGGCAAACGTTGCCA
CAATATGCTTCCAATGGCTTCAATTATCCGGCGGATAACGATCATCACGAGAAACATCTT
AGTGTGTTCAGCCGTAAGGCTCCGTATAGTTACGGTTGGGACTGGGGCATCCGAATGGTG
ACAAGTGGTGTCTGGCGCCCGGTTACTCTTCGTTTTTACGACATAGCTACCATATCCGAC
TACTATGTCAGACAATTATCGCTGACAGATGAAAACGCTCGTCTGTCTAATGAGCTGATT
GTAAACCAGATCGTTCCTCAGAAAATTCCGGCTGAAGTAAGGGTCAATGTATCGTTGAAT
GGAACTACCGTTACAGAAGTAAAACAGCAGGTGACACTGCAACCCGGGATAAATCATATC
ACTCTTCCTGCTGAAGTGACGAACCCTGTACGATGGATGCCAAATGGTTGGGGAACTCCG
ACTCTGTATGATTTCTCGGCTCAGATAGCTTGTGGAGATCGGATTGTTGCGGAACAATCT
CATCGGATTGGTTTACGAACGATTCGTGTGGTAAATGAAAAAGATAAGGACGGAGAATCT
TTTTATTTTGAAGTCAATGGCATACCGATGTTTGCCAAAGGAGCTAATTATATTCCTCAG
GATGCACTGCTGCCTAATGTTACTACGGAACGCTATCAGACTTTGTTCCGTGACATGAAG
GAGGCCAATATGAATATGGTTCGTATATGGGGTGGTGGGACATATGAGAATAATCTTTTC
TATGACCTTGCCGATGAGAACGGGATACTTGTCTGGCAGGATTTTATGTTTGCGTGTACC
CCTTATCCATCCGATCCTACTTTCCTGAAACGGGTAGAGGCGGAGGCTGTTTACAATATT
CGTCGTCTTCGGAATCATGCCTCTTTGGCGATGTGGTGTGGTAATAATGAAATACTGGAA
GCTCTGAAATATTGGGGTTTTGAGAAGAAGTTTACTCCGGAGGTTTATCAAGGATTAATG
CATGGTTATGATAAACTGTTCCGTGAGCTGTTGCCTTCAACGGTTAAAGAATTTGATTCG
GATCGTTTTTATGTGCACAGTTCGCCTTATCTCGCTAACTGGGGGCGTCCGGAGTCATGG
GGGACAGGAGACAGTCATAATTGGGGAGTCTGGTACGGAAAGAAGCCATTTGAGTCTTTA
GATACCGATTTGCCGCGTTTTATGAGTGAATTTGGTTTCCAGTCATTTCCGGAGATGAAA
ACTATTGCTGCTTTTGCTGCTCCTGAAGATTATCAGATTGAGTCTGAAGTGATGAATGCG
CATCAGAAGAGTAGTATTGGTAATTCGTTGATCCGTACTTATATGGAACGTGATTATATC
ATTCCCGAAAGTTTCGAGGATTTTGTGTATGTCGGATTAGTCTTGCAAGGACAGGGGATG
CGGCATGGACTGGAAGCTCACAGACGCAATCGTCCTTATTGTATGGGGACATTGTATTGG
CAGTTGAACGACAGTTGGCCGGTAGTATCCTGGTCAAGTATTGATTATTATGGTAATTGG
AAGGCATTACATTATCAGGCAAAGCGTGCTTTTGCACCGGTCTTGATCAATCCTATACAG
CAGAATGACAGTTTGAGTGTCTATCTGATCTCAGACCGGCTTGATACAATGGAGCAAATG
ACATTGGAAATGAAAGTCGTGGATTTTGATGGAAAAACGCTGGGTAAGAAGATACAAGTA
CATTCGTTGGAAGTTCCGGCAAATACTTCAAAATGTGTTTATCGTGCAAAACTGGATGGC
TGGCTGACTCCCGAAGACTGCCGACGTAGCTTCTTGAAATTGATACTCAAAGATAAATCG
GGACATCAGGTGGCTGAGAGCGTCCATTTTTTCAGAAAGACGAAAGATTTGCAACTTCCT
CCGACATCAGTATCCTATCAAATGAAGCAGACGGATGGTAAATGTGAATTGACTCTCTTT
TCATCAATGTTGGCAAAAGATATTTTTATTGAGACTCCATTGCAAGGAGCCCGTTACAGT
GACAACTTCTTTGATCTCTTACCCGGAGAACGTAAGAAAGTAATTATCACTTCTCCCCGG
ATAAAGAAGGGAGAAGAATTACCTGTCAATATCAAGCATATTCGAGAAACCTATAAATAG

Protein sequence predicted based on nucleotide sequence

QGNDTSEVMLLDTGWEFSQSGTEKWMPATVPGTVHQDLISHELLPNPFYGMNEKKIQWVE
NEDWEYRTSFIVSEEQLNRDGIQLIFEGLDTYADVYLNGSLLLKADNMFVGYTLPVKSVL
RKGENHLYIYFHSPIRQTLPQYASNGFNYPADNDHHEKHLSVFSRKAPYSYGWDWGIRMV
TSGVWRPVTLRFYDIATISDYYVRQLSLTDENARLSNELIVNQIVPQKIPAEVRVNVSLN
GTTVTEVKQQVTLQPGINHITLPAEVTNPVRWMPNGWGTPTLYDFSAQIACGDRIVAEQS
HRIGLRTIRVVNEKDKDGESFYFEVNGIPMFAKGANYIPQDALLPNVTTERYQTLFRDMK
EANMNMVRIWGGGTYENNLFYDLADENGILVWQDFMFACTPYPSDPTFLKRVEAEAVYNI
RRLRNHASLAMWCGNNEILEALKYWGFEKKFTPEVYQGLMHGYDKLFRELLPSTVKEFDS
DRFYVHSSPYLANWGRPESWGTGDSHNWGVWYGKKPFESLDTDLPRFMSEFGFQSFPEMK
TIAAFAAPEDYQIESEVMNAHQKSSIGNSLIRTYMERDYIIPESFEDFVYVGLVLQGQGM
RHGLEAHRRNRPYCMGTLYWQLNDSWPVVSWSSIDYYGNWKALHYQAKRAFAPVLINPIQ
QNDSLSVYLISDRLDTMEQMTLEMKVVDFDGKTLGKKIQVHSLEVPANTSKCVYRAKLDG
WLTPEDCRRSFLKLILKDKSGHQVAESVHFFRKTKDLQLPPTSVSYQMKQTDGKCELTLF
SSMLAKDIFIETPLQGARYSDNFFDLLPGERKKVIITSPRIKKGEELPVNIKHIRETYK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable