Sde_3603 (S. degradans 2-40 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  bgl1A
Gene Name:  putative retaining b-glycosidase
Original Clone ID:
BGH01_B01a2
Keyword:
full-length CDS
Species:
Saccharophagus degradans 2-40
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
SdCD00405601
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1383nts
Open reading frame: 1 to 1383

AAAACCTTTAACCCAGATTTCGTATGGGGAGCAGCCAGTTCCGCCTATCAGGTAGAAGGC
GCCACCACCACCGATGGCAGAGGCCCCAGTATTTGGGATGCGTTCAGTTCCATTCCCGGT
AAAACCTACCACAACCAAAACGCCGACATAGCCTGCGACCACTACAACCGCTGGCAAGAA
GACGTGGCCATAATGAAAGAGATGGGGCTAAAGGCTTACCGCTTTTCTATTTCTTGGTCG
CGCATATTCCCTACTGGGCGCGGCGAAGTTAACGAAAAAGGCGTAGCCTTTTACAACAAC
CTTATCGACGAATTAATAAAAAACGACATTACCCCTTGGGTAACCCTATTTCACTGGGAC
TTTCCTCTGGCACTGCAAATGGAAATGGACGGCCTACTTAACCCCGCCATCGCCGACGAA
TTCGCCAACTACGCCAAGCTGTGTTTCGCGCGCTTTGGCGACCGCGTTACCCACTGGATT
ACCCTAAACGAACCTTGGTGCAGTGCCATGCTTGGCCACGGCATGGGCAGCAAAGCCCCT
GGCCGCGTATCTAAGGATGAACCCTATATAGCCGCCCACAACTTGCTGCGTGCACACGGC
AAAATGGTAGATATTTACCGGCGCGAATTTCAGCCCACACAAAAAGGCATGATAGGCATA
GCCAACAATTGCGACTGGCGCGAACCCAAAACCGATTCTGAATTAGATAAAAAAGCAGCC
GAGCGCGCCCTAGAATTTTTTGTAAGCTGGTTTGCCGACCCCATTTATTTGGGCGACTAC
CCAGCCAGCATGCGCGAGCGCTTGGGTGAGCGTTTACCCACCTTTAGCGACGAAGACATT
GCGCTAATAAAAAACTCTAGCGACTTTTTTGGTTTGAATCACTACACCACCATGCTTGCC
GAACAAACCCACGAAGGTGACGTTGTTGAAGATACTATTCGCGGCAACGGCGGCATATCG
GAAGACCAAATGGTCACCCTCTCCAAAGACCCAAGCTGGGAACAAACCGACATGGAGTGG
AGCATTGTGCCCTGGGGCTGTAAAAAATTATTAATCTGGTTAAGCGAGCGCTACAACTAC
CCCGACATTTACATTACCGAAAACGGCTGCGCCCTACCCGACGAAGACGACGTAAACATA
GCCATTAACGATACACGCCGCGTAGATTTTTACCGCGGTTATATCGATGCGTGTCACCAA
GCAATAGAGGCCGGCGTAAAACTAAAAGGCTATTTTGCATGGACACTTATGGATAACTAC
GAATGGGAAGAAGGCTACACCAAACGCTTTGGCTTAAACCATGTAGATTTCACCACAGGC
AAACGCACACCTAAACAGTCTGCAATTTGGTATAGCACGTTAATTAAAGATGGTGGGTTC
TAG

Protein sequence predicted based on nucleotide sequence

KTFNPDFVWGAASSAYQVEGATTTDGRGPSIWDAFSSIPGKTYHNQNADIACDHYNRWQE
DVAIMKEMGLKAYRFSISWSRIFPTGRGEVNEKGVAFYNNLIDELIKNDITPWVTLFHWD
FPLALQMEMDGLLNPAIADEFANYAKLCFARFGDRVTHWITLNEPWCSAMLGHGMGSKAP
GRVSKDEPYIAAHNLLRAHGKMVDIYRREFQPTQKGMIGIANNCDWREPKTDSELDKKAA
ERALEFFVSWFADPIYLGDYPASMRERLGERLPTFSDEDIALIKNSSDFFGLNHYTTMLA
EQTHEGDVVEDTIRGNGGISEDQMVTLSKDPSWEQTDMEWSIVPWGCKKLLIWLSERYNY
PDIYITENGCALPDEDDVNIAINDTRRVDFYRGYIDACHQAIEAGVKLKGYFAWTLMDNY
EWEEGYTKRFGLNHVDFTTGKRTPKQSAIWYSTLIKDGGF*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable