jhp0765 (H. pylori J99 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  jhp0765
Gene Name:  lipopolysaccharide biosynthesis protein
Original Clone ID:
BGT25_T02c
Keyword:
full-length CDS
Species:
Helicobacter pylori J99
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HpCD00405605
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 819nts
Open reading frame: 1 to 819

CGTGTTTTTATCATTTCTTTAAATCAAAAAGTGTGCGATAAATTTGGTTTGGTTTTTAGA
GACACCACAACTTTACTCAATAATATCAACGCCACCCACCACCAAGCGCAAATTTTTGAT
GCGATTTATTCCAAGACTTTTGAAGGCGGGTTGCACCCCCTAGTTAAAAAGCATTTACAC
CCTTATTTCATCACGCAAAATATCAAAGACATGGGGATTGCAACCAGTCTCATCAGTGAG
GTTTCTAAGTTTTATTACGCTTTAAAATACCATGCGAAATTTATGAGCTTGGGGGAGCTT
GGGTGCTATGCGAGCCATTATTCGTTGTGGCAAAAATGCATAGAGCTCAATGAAGCGATC
TGTATTTTAGAAGACGATATAACCTTGAAAGAGGATTTTAAAGAGGGCTTGGATTTTTTA
GAAAAACACATCCAAGAGTTAGGCTATGCGCGTTTGATGCATTTATTGTATGATGCCAGC
GTGAAAAGTGAGCCTTTAAACCATAAAAACCAAGAGATACAAGAGCGTGTGGGGATCATT
AAAGCTTATAGCCATGGGGTGGGGACGCAAGGCTATGTGATCACGCCCAAGATTGCCAAA
GTTTTTTTGAAACACAGCCGAAAATGGGTTGTTCCTGTGGATACGATAATGGACGCTACT
TTTATCCATGGCGTGAAAAATCTGGTGTTACAACCTTTTGTGATCGCTGATGATGAGCAA
ATCTCTACGATAGCGCGAAAAGAAGAACCCTATAGCCCTAAAATCGCTCTAATGAGAGAA
CTCCATTTTAAATTTTTGAAATGGTGGCAGTTTGTATAG

Protein sequence predicted based on nucleotide sequence

RVFIISLNQKVCDKFGLVFRDTTTLLNNINATHHQAQIFDAIYSKTFEGGLHPLVKKHLH
PYFITQNIKDMGIATSLISEVSKFYYALKYHAKFMSLGELGCYASHYSLWQKCIELNEAI
CILEDDITLKEDFKEGLDFLEKHIQELGYARLMHLLYDASVKSEPLNHKNQEIQERVGII
KAYSHGVGTQGYVITPKIAKVFLKHSRKWVVPVDTIMDATFIHGVKNLVLQPFVIADDEQ
ISTIARKEEPYSPKIALMRELHFKFLKWWQFV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable