PMN2A_1233 (P. marinus str. NATL2A )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PMN2A_1233
Gene Name:  TPR repeat-containing protein
Original Clone ID:
BGT41_T01a2
Keyword:
partial CDS
Species:
Prochlorococcus marinus str. NATL2A
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
PmCD00405608
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1527nts
Open reading frame: 1 to 1527

ACCGAAGAAAGAAAGAATCAAAAGCAAGAAGGCTCTAAAGTAAAAACATTCCCAGTCTCT
TTTGCTTTAGGTGAAATTAAAGAAAACATCACTATTACGACTAATAGCGCTTCTAATACT
TCAAAGGAACAAAAAAGATTTGGAGACCAGAGTAAAACTATAAAGAAAAAAGATACCAAT
ACAATTACTAAACCTTCCAAAGATCAAATCATTAATCAAGCATTTAAATTTCATTCACAA
GGCAATATAAAAGAAGCAGCAAAAAATTATCAATATTTCATTAATCAAGGTTTCTCTGAC
CACATGGTTTTTTCTAATTATGGAGCAATATTAAGAGATCTTGGTAATTTACAAGATGCA
GAATTATATACTCGCAAAGCAATTAAAATTAATCCTAATTACGCATTGGCTTACTCTAAT
CTGGGAAATGTATTAAAAGATCTTGGTAAGTCACAAGATGCAGAATTGTCATACCGCAAA
GCAATTCAAATTAATCCTAATTACGCAGATGCACATTACAATCTGGGAATAATATTGAAA
GAACTTGGTAATTTACAAGACGCAGAATTGTCATATCGCAAAGCAATTCAAATTAATCCT
AATTACGCAGATGCATATTCCAATCTGGGAAATGTATTAAAAGATCTTGATAATTTACAA
GACGCAGAATTGTCATACCGCAAAGCAATTCAAATTAATCCTAGTTACGCAGACGCATAT
TCAAATCTGGGAAATGTATTAAAAGATCTTGGTAATTTACAAGACGCAGAGTTGTCATAC
CGCAAAGCAATTCAAATTAATCCTGATTACGCAGAGGCGCATTTCAATCTAGGAAATCTA
TTAAAAGATCTTGGTAAATTACAAGACGCAGAGTTGTCATATCGCAAAGCAATTCAAATC
AAATCTGATTACGCTGAGGCGCATTACAATCTAGGAATCATATTGAAAGACCTTGGTAAT
TTACAAGACGCAGAATTTTATAATCGCAAAGCAATTCAAATCAAACCTGATTACGCTGAG
GCGCATTTCAATCTGGGAATCATATTGAAAGACCTTGGTAATTTACAAGACGCAGAATTC
TCATATCGCCAAGCAATTCAAATCAAACCTGATTACGCAGATGCCTACTCCAATCTGGGA
AATGTATTAAAAGATCTTGGTAAGTTAAAAGACGCAGAATTGTCATATCGCAAAGCAATT
CAAATCAAACCTGATTACGCTGAGGTTTATTCCAATCTGGGAAATGTATTAAAAGATCTT
GGTAATTTACAAGACGCAGAATTTTCATACCGCAAAGCAATTCAAATCAAACCTGATTAC
GCAGATGCTTACTCCAATCTGGGAAATATATTAAAAGAGCTAAGTAATTTCACTGACGCT
ATAAATCAATTCAAGGATGCACTAAAATTGAACAATGAATTAACATCAGCTCAGACTGGT
TTAATGTCAACTCAGGGTAATATATGCGATTGGAGTGATGAGGAGACTCATAATAAATGG
CTTAAATCACTTGGTATTAAAGGATAG

Protein sequence predicted based on nucleotide sequence

TEERKNQKQEGSKVKTFPVSFALGEIKENITITTNSASNTSKEQKRFGDQSKTIKKKDTN
TITKPSKDQIINQAFKFHSQGNIKEAAKNYQYFINQGFSDHMVFSNYGAILRDLGNLQDA
ELYTRKAIKINPNYALAYSNLGNVLKDLGKSQDAELSYRKAIQINPNYADAHYNLGIILK
ELGNLQDAELSYRKAIQINPNYADAYSNLGNVLKDLDNLQDAELSYRKAIQINPSYADAY
SNLGNVLKDLGNLQDAELSYRKAIQINPDYAEAHFNLGNLLKDLGKLQDAELSYRKAIQI
KSDYAEAHYNLGIILKDLGNLQDAEFYNRKAIQIKPDYAEAHFNLGIILKDLGNLQDAEF
SYRQAIQIKPDYADAYSNLGNVLKDLGKLKDAELSYRKAIQIKPDYAEVYSNLGNVLKDL
GNLQDAEFSYRKAIQIKPDYADAYSNLGNILKELSNFTDAINQFKDALKLNNELTSAQTG
LMSTQGNICDWSDEETHNKWLKSLGIKG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable