mlr5848 (M. japonicum MAFF 303099 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  mlr5848
Gene Name:  nodulation protein NodZ
Original Clone ID:
BGT23_T02b
Keyword:
full-length CDS
Species:
Mesorhizobium japonicum MAFF 303099
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
MlCD00405618
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 987nts
Open reading frame: 1 to 987

CTTGTTGATCGCTCAAGGAACGATCGGTTCGTTGTTTCTCGACGGCGCACGGGTCTCGGT
GATTGCCTGTGGTCTCTCGCGTCGGCTTGGGACTACGCACATCGGACGGGACGAGCTTTA
GCAATAGATTGGCGCGGCTCTTGTTACCTTGACAACCCGTTTGCGAATGCCTTTCCAGCT
TTCTTCAAGCCAATTGAGGATATCGGTGGTGTACGGGTCATTTGCGATGACCAGATCAAC
CACGTCTCATTCCCTGGTCCCTTCTTCCCGTCGTGGTGGAACAAGCCATCCATCGACTGC
GTGTACCGTCCGGATGAACAGATTTTCCAAGAACGTGACGAGCTTAACGATCTTTTCCAG
GCCCAAGATGATTGCGAAGCCAACACGGTAGTGTGTGATGCTTGCTTGATGTGGCGCTGC
GATGAAAAGGCCGAGCGAACCATATTTAGGAGCATCACACCGAGGGTCGAAATTGAGGAT
CGCATTGAAGCGCTCTACCAGGAACATTTTGACGGGCACAGCATAATTGGAGTCCATGTC
CGCCACGGCAACGGCGAAGATATTATGGATCACGCACCTTACTGGGCTGATCCGAAGGTT
GCCTTAGAGCAGGTATGCACTGCCATTCGTATGGCCAAGGCGCAACCGCATTCAAGACCT
GTAAGGGTGTTCTTGTGTACAGACAGCGCCAAGGTTGTTGATCACCTGTCGGGCGTATTT
CCCGAACTTTTCACCGTCCCAAAACGCTTCCAGGCGGATCAGGCCGGCCCCTTACACAGT
GCAGCACTGGGCACCGACGGGGGGGGTTCCGCGCTCGTTGAGATGTATCTCCTCGGTCGA
TGCGACACTGTTATTCGCTTTCCTCCGACTAGCGCCTTCACCCGCTACGCCCGCCTCTTC
GTGCCACGAATTGTCGAATTCGATTTGAATGACCCGCGTCGTTTGATCTTGATCGACAAC
ACTTTCGAACGTTCCCCGGCTCCATAG

Protein sequence predicted based on nucleotide sequence

LVDRSRNDRFVVSRRRTGLGDCLWSLASAWDYAHRTGRALAIDWRGSCYLDNPFANAFPA
FFKPIEDIGGVRVICDDQINHVSFPGPFFPSWWNKPSIDCVYRPDEQIFQERDELNDLFQ
AQDDCEANTVVCDACLMWRCDEKAERTIFRSITPRVEIEDRIEALYQEHFDGHSIIGVHV
RHGNGEDIMDHAPYWADPKVALEQVCTAIRMAKAQPHSRPVRVFLCTDSAKVVDHLSGVF
PELFTVPKRFQADQAGPLHSAALGTDGGGSALVEMYLLGRCDTVIRFPPTSAFTRYARLF
VPRIVEFDLNDPRRLILIDNTFERSPAP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable