aga27A (C. japonicus Ueda107 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  aga27A
Gene Name:  putative alpha-galactosidase, aga27A
Original Clone ID:
BGH27_B01c
Keyword:
partial CDS
Species:
Cellvibrio japonicus Ueda107
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
CjCD00405628
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1146nts
Open reading frame: 1 to 1146

CAAAAATTTGAGCAACTCGCTAAAACACCGCAAATGGGTTGGAATAGCTGGAATACCTTC
GGCTGCAACGTGGATGAAAAAATGATTCGCGCAATGGCCGATGCCATGGTCACATCCGGT
ATGAAGGCTGCCGGTTATGAATACATTAATATTGACGATTGCTGGCACGGCGAGCGCGAT
AAAAATGGCTTTATCCAGGCGGATAAAAAACATTTCCCTTCGGGCATGAAAGCACTTGCT
GATTACGTGCATGCCAAAGGTTTGAAATTGGGGATTTATTCCGATGCCGGTAATACCACC
TGTGCCGGCCGCCCCGGCAGTCGCGGCCATGAATACCAGGACGCACTGACCTACGCGAGT
TGGGGTATCGATTACGTAAAATACGATTGGTGCGATACCCAGGATATTAATCCCAAGTCC
GCCTACGCCACCATGCGCGATGCCATCCATAAAGCGGGCCGCCCCATGTTGTTCAGTATT
TGCGAATGGGGCGATAACCAGCCCTGGGAATGGGCGCAGGATGTCGGCCATTCCTGGCGC
ACAACCGGCGACATCTATCCCTGTTGGAACTGCGAACACAACCACGGCTCCTGGTCTTCC
TTTGGGGTGTTGCCCATCCTCGACAAACAAGCGGGCTTGCGTAAATACGCCGGCCCCGGC
CACTGGAATGACATGGATATGATGGAAGTGGGCAACGGCATGACCGAGGAAGAAGATCGC
GCGCATTTCTCACTCTGGGCATTTATGGCATCACCGCTGATTGCCGGCAATGACCTGCGC
AACATGAGCGACACCACCCGCGCTATCCTGACCCACAAGGAAACCATTGCGATTAACCAG
GACAAACTGGGCATCCAGGCCATGAAGTGGATCGATGAAGGCGACCTGGAGATTTATATC
AAGCCATTGGAAAAAGGCCATTACGCTGTGCTGTTCCTCAATCGTGCCGATGATGCCATG
GACTATCGTTTTGACTGGTCTTTCCATTACATGAAAGACGATATCAGCAAGCACGAAATT
TTCTTTGATAAGCAGGCCTTTAACTGGCGCAATATCTGGAATGGTGAAACAGGCTCTACA
AAAGAGGTGCTGAATATCAAGGTGCCAGCGCACGGTGTCGTGGTATTGCGTTTAAGCCCG
CGTTAG

Protein sequence predicted based on nucleotide sequence

QKFEQLAKTPQMGWNSWNTFGCNVDEKMIRAMADAMVTSGMKAAGYEYINIDDCWHGERD
KNGFIQADKKHFPSGMKALADYVHAKGLKLGIYSDAGNTTCAGRPGSRGHEYQDALTYAS
WGIDYVKYDWCDTQDINPKSAYATMRDAIHKAGRPMLFSICEWGDNQPWEWAQDVGHSWR
TTGDIYPCWNCEHNHGSWSSFGVLPILDKQAGLRKYAGPGHWNDMDMMEVGNGMTEEEDR
AHFSLWAFMASPLIAGNDLRNMSDTTRAILTHKETIAINQDKLGIQAMKWIDEGDLEIYI
KPLEKGHYAVLFLNRADDAMDYRFDWSFHYMKDDISKHEIFFDKQAFNWRNIWNGETGST
KEVLNIKVPAHGVVVLRLSPR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable