BCE_2168 (B. cereus ATCC 10987 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  BCE_2168
Gene Name:  glycosyltransferase, MGT family
Original Clone ID:
BGT01_T01a
Keyword:
full-length CDS
Species:
Bacillus cereus ATCC 10987
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
BhCD00405631
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1200nts
Open reading frame: 1 to 1200

GCAAACGTACTCGTAATAAATTTCCCTGGAGAAGGTCATATAAATCCGACTTTAGCTATT
GTAAGTGAGTTAATTCGGCGAGGGGAGACAGTTGTTTCGTATTGTATTGAAGATTATAGA
AAGAAGATTGAAGCAACAGGTGCACAATTCCGAGTGTTTGAGAATTTCCTCTCTCAAATT
AATATTATGGAGCGAGTAAATGAAGGTGGGAGTCCTTTGACGATGCTGTCTCATATGATG
GAAGCATCAGAACGTATTGTTACTCAAATTGTAGAAGAAACAAAAGGGGAAAAGTACGAT
TATTTGATATATGATAATCACTTTCCAGTAGGACGTATTATAGCCAATGTTTTAAAGTTA
CCTAGTGTTTCTTCTTGTACAACGTTTGCTTTTAATCAGTACATTACTTTTAACGATGAA
CATGAATCAAGAGAAGTAGATGAAACGAATCCATTGTATCAATCTTGTTTAGCGGGAATG
GAAAAATGGAACAAACAGTATGGAATGAAATGTAATAGTATGTATGATATTATGAACCAT
CCTGGTGATATTACAATTGTGTATACTTCAAAGGAATATCAGCCGCGTTCAGATGTATTC
GATGAATCGTATAAGTTTGTTGGCCCATCAATTGCTACTCGAAAAGAAGTAGGTAGCTTT
CCTATGGAAGATTTAAAAGATGAAAAATTGATTTTCATTTCTATGGGAACAGTTTTTAAT
GAACAACCTGAGTTATATGAAAAATGTTTTGAAGCGTTTAAAGATGTAGAAGCGACAGTC
GTATTAGTTGTTGGTAAGAAGATAAATATAAGTCAATTTGAAAACATTCCGAATAACTTT
AAGTTGTATAATTATGTGCCGCAATTAGAACTATTACAGTATGCTGATGTATTCGTAACA
CACGGCGGTATGAATAGTTCAAGTGAAGCACTATATTACGGTGTCCCGTTAGTTGTAATT
CCGGTAACAGGAGATCAGCCTTTAGTTGCGAAACGAGTAAATGAAGTAGGGGCTGGAATA
AGGCTTAATCGCAAAGAATTAACTTCTGAAATGTTACGTGAGTCTGTAAAGAAAGTGATG
GATGATGTAACGTTTAAGGAAAAAAGTCGTAAAGTTGGAGAGTCACTTCGAAATGCTGGT
GGTTATAATAGGGCAGTTGATGAAATATTAAAAATGAATTCATACTCAAAACTTAAATAG

Protein sequence predicted based on nucleotide sequence

ANVLVINFPGEGHINPTLAIVSELIRRGETVVSYCIEDYRKKIEATGAQFRVFENFLSQI
NIMERVNEGGSPLTMLSHMMEASERIVTQIVEETKGEKYDYLIYDNHFPVGRIIANVLKL
PSVSSCTTFAFNQYITFNDEHESREVDETNPLYQSCLAGMEKWNKQYGMKCNSMYDIMNH
PGDITIVYTSKEYQPRSDVFDESYKFVGPSIATRKEVGSFPMEDLKDEKLIFISMGTVFN
EQPELYEKCFEAFKDVEATVVLVVGKKINISQFENIPNNFKLYNYVPQLELLQYADVFVT
HGGMNSSSEALYYGVPLVVIPVTGDQPLVAKRVNEVGAGIRLNRKELTSEMLRESVKKVM
DDVTFKEKSRKVGESLRNAGGYNRAVDEILKMNSYSKLK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable