BT_3590 (B. thetaiotaomicron VPI-5482 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_3590
Gene Name:  alpha-N-acetylglucosaminidase
Original Clone ID:
BGH89_B01b
Keyword:
partial CDS
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
BtCD00405635
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 2148nts
Open reading frame: 1 to 2148

CAACGCAATGTAGAAACAGGCCCTCCTGTATTAAAAGAAATGTGCCAAAGATTATTTCCC
CGGCATGCCCAGTCGTTTCTATTCGAGTTACTGACAGATTCTATTGATACAGACCGTTTT
ATACTGGAATCGAGTCAAGGGAAAATACGGATAAAAGGAAACAATCGTAACTCATTGGCT
GCCGGTTTGAATCATTATCTGAAAAACTACTGTCATACCCATGTTTCCTGGTATGCCTCA
GAGACAGTGGAAATGCCTGATGTACTACCGGAGATACCTCAGCCAGTATATATCCGTTCC
AAATGTGACAATCGTTTTTTTCTCAACTATTGTACATTCGGTTATACTATGCCCTATTGG
AAATGGCAGGACTGGGAACGACTGATAGACTGGATGGCATTAAACGGAGTCACCATGCCT
CTTGCTATTACCGGACAAGAATCCATTTGGTACAAAGTATGGACAGACATGGGTTTGAGC
GACGAACAGGTTCGCTCATACTTTACAGGGCCAGCCCATCTTCCCTGGCATCGGATGTCC
AATGTCGATTTCTGGCAAAGTCCACTACCTCAATCCTGGCTAAAGGATCAGGAGGAATTA
CAAAAACGTATTCTCGAAAGAGAACGTGAGTTTGATATGACTCCTGTATTACCAGCATTT
GCCGGTCATGTACCAGCCGAATTAAAAACAATTTATCCGAATGCAAAAATCTATCAGATG
AGTCAGTGGGGTGGCTTCGATGAAAAATACCGCAGTCATTTTATTGATCCGATGGACTCA
TTATACTCAATCATTCAGCGCCGTTTTCTGGAGGAACAAACCAAAGTATATGGTACTGAC
CATATTTATGGAATCGATCCTTTCAATGAAGTCGACTCTCCTGACTGGAGCGAGGATTTT
TTAGCCAATGTTTCAAGCAAGATTTACGAGTCGATTCATCAGGTAGACTCAGCAGCCCAA
TGGTTACAAATGACCTGGATGTTCTTCTATGACAAAAAGAAATGGACTCAACCCCGTATC
CGTTCCTTTCTAAAAGCAGTACCGGATAACAAACTGATATTACTCGATTACTATTGCGAT
CATACAGAAATCTGGCGAAATACTGAAAAATATTACGGGAATCCCTATATTTGGTGTTAT
CTGGGTAATTTTGGCGGCAATACGATGATAGCAGGCAATCTGAATGATATTGATTTCAAG
ATTAAACGACTCTTCAAGGAGGGAGGAGACAATGTCTATGGCTTGGGAGCAACTCTTGAA
GGCTTTGATGTAAACCCTCTTATGTACGAATTTGTTTTTGACCAGGCATGGGATTATCCT
GTCACTACCGACCAATGGATAACCAACTGGTCCATGTGCAGAGGAGGAAATCAGGATGCC
AATATCATCAAAGCGTGGAGAGCCTTGCATCAGAAAATTTATACCGAACATGCCACATGC
GGCCAATCGGTATTAATGAACGCACGCCCCAGACTAACGGGAACTAAAAGTTGGAATACA
AACCCCGGCATACATTACGCAAATAACGACTTATGGCAAATATGGAAAGAGCTACTAAAA
GCCCGGAACATCAACAATAGTGATTTTCGCTTTGACGTAATCAATATCGGAAGACAAGTA
CTAGGAAACTTGTTCTCCGAATATAGAGATCAGTTTACCGCTTGCTATAACCGGAAAGAT
ACAACAGGCATGAGAGAATGGAGTACCCGTATGGACAACCTCTTGCTTGACGTAGACCGC
CTCCTTTCCTGTGACGCGACTCTATCCATCGGGAAATGGTTACAAGACGCCAGAAACTGT
GGTGCAACCGTGTCGGAAAAGGATTACTATGAAGAAAATGCACGCTGTATCCTGACCGTA
TGGGGGCAACAGGACACCCAACTGAATGACTATGCCAATCGAGGCTGGGGCGGACTTACC
CGAAGCTTCTACCGGGAACGTTGGAAACGTTTCACAGACGGAGTCATCGCTGCTGTTTCG
GAAGATAAACCTTTTGACGAAGACAAATTCCACCAGGACATTACGCAATTTGAATACAAC
TGGACATTACAGAAAGACTCTTTCCCTATTGTTTCAGAGGAAGACCCGATTCAAATAGCA
GACAGTCTTATCTTAAAATACGATACGTACTTTACCAAAGCACAATAG

Protein sequence predicted based on nucleotide sequence

QRNVETGPPVLKEMCQRLFPRHAQSFLFELLTDSIDTDRFILESSQGKIRIKGNNRNSLA
AGLNHYLKNYCHTHVSWYASETVEMPDVLPEIPQPVYIRSKCDNRFFLNYCTFGYTMPYW
KWQDWERLIDWMALNGVTMPLAITGQESIWYKVWTDMGLSDEQVRSYFTGPAHLPWHRMS
NVDFWQSPLPQSWLKDQEELQKRILEREREFDMTPVLPAFAGHVPAELKTIYPNAKIYQM
SQWGGFDEKYRSHFIDPMDSLYSIIQRRFLEEQTKVYGTDHIYGIDPFNEVDSPDWSEDF
LANVSSKIYESIHQVDSAAQWLQMTWMFFYDKKKWTQPRIRSFLKAVPDNKLILLDYYCD
HTEIWRNTEKYYGNPYIWCYLGNFGGNTMIAGNLNDIDFKIKRLFKEGGDNVYGLGATLE
GFDVNPLMYEFVFDQAWDYPVTTDQWITNWSMCRGGNQDANIIKAWRALHQKIYTEHATC
GQSVLMNARPRLTGTKSWNTNPGIHYANNDLWQIWKELLKARNINNSDFRFDVINIGRQV
LGNLFSEYRDQFTACYNRKDTTGMREWSTRMDNLLLDVDRLLSCDATLSIGKWLQDARNC
GATVSEKDYYEENARCILTVWGQQDTQLNDYANRGWGGLTRSFYRERWKRFTDGVIAAVS
EDKPFDEDKFHQDITQFEYNWTLQKDSFPIVSEEDPIQIADSLILKYDTYFTKAQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable