malP (E. coli str. K-12 substr. MG1655 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  malP
Gene Name:  maltodextrin phosphorylase
Original Clone ID:
BGT35_T01b
Keyword:
full-length CDS
Species:
Escherichia coli str. K-12 substr. MG1655
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
EcCD00405638
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 2391nts
Open reading frame: 1 to 2391

TCACAACCTATTTTTAACGATAAGCAATTTCAGGAAGCGCTTTCACGTCAGTGGCAGCGT
TATGGCTTAAATTCTGCGGCTGAAATGACTCCTCGCCAGTGGTGGCTAGCAGTGAGTGAA
GCACTGGCCGAAATGCTGCGTGCTCAGCCATTCGCCAAGCCGGTGGCGAATCAGCGACAT
GTTAACTACATCTCAATGGAGTTTTTGATTGGTCGCCTGACGGGCAACAACCTGTTGAAT
CTCGGCTGGTATCAGGATGTACAGGATTCGTTGAAGGCTTATGACATCAATCTGACGGAC
CTGCTGGAAGAAGAGATCGACCCGGCGCTGGGTAACGGTGGTCTGGGACGTCTGGCGGCG
TGCTTCCTCGACTCAATGGCAACTGTCGGTCAGTCTGCGACGGGTTACGGTCTGAACTAT
CAATATGGTTTGTTCCGCCAGTCTTTTGTCGATGGCAAACAGGTTGAAGCGCCGGATGAC
TGGCATCGCAGTAACTACCCGTGGTTCCGCCACAACGAAGCACTGGATGTGCAGGTAGGG
ATTGGCGGTAAAGTGACGAAAGACGGACGCTGGGAGCCGGAGTTTACCATTACCGGTCAA
GCGTGGGATCTCCCCGTTGTCGGCTATCGTAATGGCGTGGCGCAGCCGCTGCGTCTGTGG
CAGGCGACGCACGCGCATCCGTTTGATCTGACTAAATTTAACGACGGTGATTTCTTGCGT
GCCGAACAGCAGGGCATCAATGCGGAAAAACTGACCAAAGTTCTCTATCCAAACGACAAC
CATACTGCCGGTAAAAAGCTGCGCCTGATGCAGCAATACTTCCAGTGTGCCTGTTCGGTA
GCGGATATTTTGCGTCGCCATCATCTGGCGGGGCGTAAACTGCACGAACTGGCGGATTAC
GAAGTTATTCAGCTGAACGATACCCACCCAACTATCGCGATTCCAGAACTGCTGCGCGTG
CTGATCGATGAGCACCAGATGAGCTGGGATGACGCCTGGGCCATTACCAGCAAAACTTTC
GCTTACACCAACCATACCCTGATGCCAGAAGCGCTGGAACGCTGGGATGTGAAACTGGTG
AAAGGCTTACTGCCGCGCCACATGCAGATTATTAACGAAATTAATACTCGCTTTAAAACG
CTGGTAGAGAAAACCTGGCCGGGCGATGAAAAAGTGTGGGCCAAACTGGCGGTGGTGCAC
GACAAACAAGTGCATATGGCGAACCTGTGTGTGGTTGGCGGTTTCGCGGTGAACGGTGTT
GCGGCGCTGCACTCGGATCTGGTGGTGAAAGATCTGTTCCCGGAATATCACCAGCTATGG
CCGAACAAATTCCATAACGTCACCAACGGTATTACCCCACGTCGCTGGATCAAACAGTGC
AACCCGGCACTGGCGGCTCTGTTGGATAAATCACTGCAAAAAGAGTGGGCTAACGATCTC
GATCAGCTGATCAATCTGGAAAAATTCGCTGATGATGCGAAATTCCGTCAGCAATATCGC
GAGATCAAGCAGGCGAATAAAGTCCGTCTGGCGGAGTTTGTGAAAGTTCGTACCGGTATT
GAGATCAATCCACAGGCGATTTTCGATATTCAGATCAAACGTTTGCATGAGTACAAACGC
CAGCACCTGAATCTGCTGCATATTCTGGCGTTGTACAAAGAAATTCGTGAAAACCCGCAG
GCTGATCGCGTACCGCGCGTCTTCCTCTTCGGCGCGAAAGCGGCACCGGGCTACTACCTG
GCGAAGAATATTATCTTTGCGATCAACAAAGTGGCTGACGTGATCAACAACGATCCGCTG
GTTGGCGATAAGTTGAAGGTGGTGTTCCTGCCGGATTATTGCGTTTCGGCGGCGGAAAAA
CTGATCCCGGCGGCGGATATCTCCGAACAAATTTCGACTGCAGGTAAAGAAGCTTCCGGT
ACCGGCAATATGAAACTGGCGCTCAATGGTGCGCTTACTGTCGGTACGCTGGATGGGGCG
AACGTTGAAATCGCCGAGAAAGTCGGTGAAGAAAATATCTTTATTTTTGGTCATACCGTG
GAACAAGTGAAGGCAATTCTGGCCAAAGGCTACGACCCGGTGAAATGGCGGAAGAAAGAT
AAGGTGCTGGACGCAGTATTGAAAGAGCTGGAAAGCGGTAAATACAGCGACGGCGATAAG
CATGCCTTCGACCAGATGCTGCACAGTATCGGCAAACAGGGCGGCGATCCGTATCTGGTG
ATGGCGGATTTCGCAGCCTATGTAGAGGCACAAAAGCAGGTGGATGTGCTGTACCGCGAC
CAGGAGGCCTGGACTCGCGCGGCGATCCTCAATACCGCCCGCTGCGGTATGTTTAGCTCG
GATCGCTCTATTCGCGATTATCAGGCTCGTATCTGGCAGGCAAAACGCTAG

Protein sequence predicted based on nucleotide sequence

SQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALAEMLRAQPFAKPVANQRH
VNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDLLEEEIDPALGNGGLGRLAA
CFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPDDWHRSNYPWFRHNEALDVQVG
IGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPLRLWQATHAHPFDLTKFNDGDFLR
AEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQCACSVADILRRHHLAGRKLHELADY
EVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAWAITSKTFAYTNHTLMPEALERWDVKLV
KGLLPRHMQIINEINTRFKTLVEKTWPGDEKVWAKLAVVHDKQVHMANLCVVGGFAVNGV
AALHSDLVVKDLFPEYHQLWPNKFHNVTNGITPRRWIKQCNPALAALLDKSLQKEWANDL
DQLINLEKFADDAKFRQQYREIKQANKVRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKR
QHLNLLHILALYKEIRENPQADRVPRVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPL
VGDKLKVVFLPDYCVSAAEKLIPAADISEQISTAGKEASGTGNMKLALNGALTVGTLDGA
NVEIAEKVGEENIFIFGHTVEQVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDK
HAFDQMLHSIGKQGGDPYLVMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSS
DRSIRDYQARIWQAKR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable