Sde_2938 (S. degradans 2-40 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Sde_2938
Gene Name:  putative amylase
Original Clone ID:
BGH13_B01b2
Keyword:
partial CDS
Species:
Saccharophagus degradans 2-40
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
SdCD00405641
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1326nts
Open reading frame: 1 to 1326

CAACCGCGAACCGCGTTCGTTCATTTGTTCGAATGGCAGTGGAACGATATTGCTAGCGAG
TGCGAAAATGTACTGGGGCCAAAAGGCTATGCGGCAGTGCAGGTATCCCCACCCCAAAAA
TCGGTTAGTAGCAGCCAATGGTGGAGTCGTTACCAACCTGTTAGCTATGCCATAGAGGGC
CGTTCTGGCAATCGCAACGAATTCGCCAGCATGGTAAGCCGGTGTAAAAATGCTGGGGTG
GATATATACGTAGATGCCGTAATTAATCATATGGCTGCCGGCGGGCGCTATTACCCAGAA
GTGCCTTACTACGCAGAGCATTTTCACACGTGCTACGGCCAAATTGATTACGGTAACCGC
TGGCAGGTACAAAACTGTGACTTGGTAGGGCTAAACGATTTAGCAACAGAACAAGACTAT
GTACGCACAAAAATAGCCAATTACTTAAACGACTTAACTAACCTTGGCGTAGCTGGCTTC
CGCTTTGATGCTGCGAAACATATCCCCGTGGGTGACATTGCTGCAATTAAAGCAAAACTT
ATTGGCAACCCGTATATATTCCAAGAAGTGATAGGTGCGCCGGGCGAGCCCATCCAACCA
TCAGAATATACCTACTTAGCAGACGTAACAGAGTTTAACTTTACCAAATCGGTTAGCCAT
TTTTTTAAAGGGCGCGGACCAATTAAAGAGTTAAAAAATATTGGTACTTGGGGCGGTTGG
GTTGCAAGTGCAGACGCCGTAACCTTTGTTGCCAACCACGATAACCAGCGCCAGGATACA
AACAACACCATTACCCATAAAGATGGTGATAACCGCAATACTATGGCGCACGTATTTATG
CTGGGCTGGCCCTATGGGTACCCTAAAGTAATGTCTAGTTATGAGTGGTACGATCACGAC
CAAGGCCCTCCCGCCCACGGTGCTAGCAGCTGTAACAGCGGCTGGTTATGTGAACACCGC
ACGCCAGCTATCGCCAATATGGTTGCTTTTCGCAATTTCACTGCAGACGAATTTCGCGTA
ACCAACTATTGGGATAACGGCAACAATCAAATAGCTTGGGGCCGAGGCGGCAAAGGGTTT
GTAGTTATAAATATGGAAGGCAACAATTTAACCCGTACATTTCAAACAGGGATGCCCGCT
GGCCGTTATTGCGACATTATTTCGGGTAACTACAACGCGTCTAATAAAAGTTGTTCTGGC
AATATTATAGATGTAGATAACAATGGCAACGCAGTAATCACCGCTTACTACCGCAACGGT
GCCGCAATTCATGTAGGCGCTATTGTTGAAGACTGTGACGGTACAGATTGCTGCACAAAC
TGCTAG

Protein sequence predicted based on nucleotide sequence

QPRTAFVHLFEWQWNDIASECENVLGPKGYAAVQVSPPQKSVSSSQWWSRYQPVSYAIEG
RSGNRNEFASMVSRCKNAGVDIYVDAVINHMAAGGRYYPEVPYYAEHFHTCYGQIDYGNR
WQVQNCDLVGLNDLATEQDYVRTKIANYLNDLTNLGVAGFRFDAAKHIPVGDIAAIKAKL
IGNPYIFQEVIGAPGEPIQPSEYTYLADVTEFNFTKSVSHFFKGRGPIKELKNIGTWGGW
VASADAVTFVANHDNQRQDTNNTITHKDGDNRNTMAHVFMLGWPYGYPKVMSSYEWYDHD
QGPPAHGASSCNSGWLCEHRTPAIANMVAFRNFTADEFRVTNYWDNGNNQIAWGRGGKGF
VVINMEGNNLTRTFQTGMPAGRYCDIISGNYNASNKSCSGNIIDVDNNGNAVITAYYRNG
AAIHVGAIVEDCDGTDCCTNC*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable