gly30A (C. japonicus Ueda107 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  gly30A
Gene Name:  glycoside hydrolase
Original Clone ID:
BGH30_B01b
Keyword:
partial CDS
Species:
Cellvibrio japonicus Ueda107
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
CjCD00405643
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1386nts
Open reading frame: 1 to 1386

ACCCAGGCCGTGGACACCGGCCCGCAAAAAACCTTGCAGGTCTACACCACCGCCAAAGAC
ACAAAATTGCGTCTCACCTTGAGCGATAAACTCTCGCTCAAACCCGCGCAACAGGCGCCG
GAAACCGACACCGCCATTTTCGTCAACCCGGACAAGCGCTACCAAACCCTGCTCGGTATT
GGCGGCGCTATCACCGATGCCAGCGCCGAGGTGTATGCAACACTCGATCAAAAAACGCAG
CGGGAATTTATGCAGGCTTACTATGACCAGGACAAGGGTCTGGGCTACAGCCTGCTGCGC
ACCACCATCCACAGCTCCGACTTTGCCAGCAGCAGCTACACCTATATCGAAGAGGGCGAC
AAAGCGCTTAACACCTTTGATATAACGCCGGATAAAAAACACCGCATCCCCATGATCAAG
CAGGCCATCCGGGCCGCCGGCGGCAGCATCACCACCTACGCCAGCCCCTGGAGCGCACCG
GCGTTTATGAAAGACACCAACAATATGCTGCAAGGCGGCAAGCTGCTGCCCGAATACCGC
GACACCTGGGCCACCTACTTCACCAAATTTATCCGCGCCTATGAGCAGGAAGGCATTCCG
ATTTGGGGGATTACCGTACAAAACGAACCCATGGCCAAGCAAACCTGGGAATCGATGATT
TTCACCGCGGAAGAAGAGCGCGATTTCCTCAAAAACCACCTGGGCCCGATCATGCACACA
TCCGGCCTGGGCGATAAAAAAATCATCGTGTGGGATCACAACCGCGACCTGATCACGCAC
CGCGCCAACACCATCCTCAGCGATCCCGAAGCGGCTAAATATGTCTGGGGCGTGGGCTTC
CACTGGTACGAAACCTGGACCGGCGGCCAACCCATGTTCGGCAACGTGGCCGAAGTCAAG
CAGGCATTCCCGCATATCAACCTGCTGCTCACCGAAGCCACGGTGGAAAAATTTGATGCG
GCGCGCTACCACCACTGGCCCAATGCCGAACGCTACGGCAACTCCATCGTCAATGACTTC
AACGCCGGCGCGGCAGGCTGGACCGACTGGAATATGCTGCTGGACGATAAAGGCGGCCCC
AACCATGTCAGCAATTTCTGCTTCGCCCCCATTCACGCCGACAGCAAAACCGGCAAGCTG
ATTTACACCCCGACCTATTACTACCTCGGCCACTTCTCGCGCTTTATCCGCCCTGAGGCG
CAGCGGGTCAGTGCGGTCAGCAGCCGCAGCGTGCTGCAAACCACGTCGTTTATCAACGCC
GATAACCAACTGGCAACGGTGGTCTTGAATTTAAGCGATGCCGCCGTGACCTATCGCTTT
TATATAGGCACACAGGAAACCACCGTTACTATCCCCGCGCGCGCCATCCAAACGCTGGTG
TATTAG

Protein sequence predicted based on nucleotide sequence

TQAVDTGPQKTLQVYTTAKDTKLRLTLSDKLSLKPAQQAPETDTAIFVNPDKRYQTLLGI
GGAITDASAEVYATLDQKTQREFMQAYYDQDKGLGYSLLRTTIHSSDFASSSYTYIEEGD
KALNTFDITPDKKHRIPMIKQAIRAAGGSITTYASPWSAPAFMKDTNNMLQGGKLLPEYR
DTWATYFTKFIRAYEQEGIPIWGITVQNEPMAKQTWESMIFTAEEERDFLKNHLGPIMHT
SGLGDKKIIVWDHNRDLITHRANTILSDPEAAKYVWGVGFHWYETWTGGQPMFGNVAEVK
QAFPHINLLLTEATVEKFDAARYHHWPNAERYGNSIVNDFNAGAAGWTDWNMLLDDKGGP
NHVSNFCFAPIHADSKTGKLIYTPTYYYLGHFSRFIRPEAQRVSAVSSRSVLQTTSFINA
DNQLATVVLNLSDAAVTYRFYIGTQETTVTIPARAIQTLVY*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable