BT_0771 (B. thetaiotaomicron VPI-5482 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_0771
Gene Name:  1,4-alpha-glucan branching enzyme
Original Clone ID:
BGH13_B05a3
Keyword:
partial CDS
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
BtCD00405644
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 621nts
Open reading frame: 1 to 621

CTGATTGATGCCGATATGTATTGGCATATGCAGAAAGGCGATGAGAATTATGTAGTCAAT
CGCGGAATCGCTTTACATAAGATGATCCGCTTGCTGACATCTTCGACGATCAATGGCGGT
TATCTGAACTTTATGGGTAATGAGTTCGGGCATCCCGAATGGATCGATTTTCCGCGTGAA
GGTAATGGATGGTCTTGTAAGTATGCCCGTCGTCAGTGGGATCTGGTAGATAACAAGAAT
CTGGCTTATCACTACATGGGGGACTTTGATGCTGAGATGCTGAAAGTGATCAAGAGTGTG
AAAGATTTCCAGGCGACTCCGGTCCAGGAGATCTGGCATAATGATGGTGATCAGGTATTG
GCTTATGGACGTAAGGATCTGATATTTGTATTTAACTTCAATCCGAAACAGTCTTTTGTA
GATTATGGTTTCCTCGTTAGTCCGGGAGCTTATGAAGTAATCTTGAATACGGATAATGTT
GCATTTGGCGGAAATGGTCTTGCAGATGATTCAGTAGTTCATTTCACGATTGCCGATCCG
TTGTATAAGAAGGAAAAGAAAGAGTGGCTGAAACTCTATATTCCTGCACGTACAGCTGTT
GTTTTGAGAAAGAAGAAATAG

Protein sequence predicted based on nucleotide sequence

LIDADMYWHMQKGDENYVVNRGIALHKMIRLLTSSTINGGYLNFMGNEFGHPEWIDFPRE
GNGWSCKYARRQWDLVDNKNLAYHYMGDFDAEMLKVIKSVKDFQATPVQEIWHNDGDQVL
AYGRKDLIFVFNFNPKQSFVDYGFLVSPGAYEVILNTDNVAFGGNGLADDSVVHFTIADP
LYKKEKKEWLKLYIPARTAVVLRKKK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable