Gene:
Gene Symbol:
BT_0290
Gene Name:
beta-galactosidase
Original Clone ID:
BGH35_B04c
Keyword:
partial CDS
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
BtCD00405649
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1890nts
Open reading frame: 1 to 1890
TCTCAATCTTCGGAGGGCACATTTGAAGTGGGGAAGAATACTTTCTTGCTGAATGGAGAA
CCGTTTGTTGTAAAAGCGGCAGAGATTCATTATCCGCGTATCCCGAAAGAATATTGGGAA
CATCGTATTAAAATGTGCAAGGCACTGGGTATGAATACGATTTGCTTGTATGTGTTCTGG
AATTTTCATGAACCGGAAGAAGGAAGGTATGATTTTGCCGGACAGAAAGATATAGCTGCG
TTTTGCCGTTTGGCTCAAGAGAATGGAATGTATGTCATAGTGCGTCCCGGACCTTATGTC
TGTGCGGAATGGGAAATGGGCGGCTTGCCTTGGTGGCTGCTGAAGAAGAAGGATATCAAA
CTTCGCGAGCAAGACCCATACTACATGGAACGTGTGAAACTCTTTTTGAACGAAGTGGGC
AAACAGCTGGCTGATCTTCAGATTAGTAAAGGAGGGAATATCATTATGGTACAGGTGGAG
AATGAGTATGGAGCATTTGGAATCGATAAGCCGTATATATCCGAAATCCGTGACATGGTA
AAACAAGCCGGTTTTACAGGTGTTCCTTTATTCCAGTGTGACTGGAACTCGAATTTTGAG
AATAATGCACTGGATGATTTGCTCTGGACTATTAATTTCGGTACGGGTGCTAACATCGAC
GAGCAATTTAAACGGTTGAAAGAACTACGGCCGGACACTCCATTGATGTGCAGTGAATTT
TGGTCCGGATGGTTTGACCATTGGGGAGCCAAGCATGAAACAAGAAGTGCCGAAGAATTG
GTAAAAGGTATGAAAGAAATGCTGGACCGCAATATTTCTTTCAGCTTGTATATGACTCAC
GGGGGGACTAGTTTCGGGCATTGGGGAGGCGCGAATTTCCCGAACTTTTCACCGACTTGC
ACATCGTATGATTATGATGCTCCTATCAATGAATCCGGTAAGGTGACTCCTAAATATCTG
GAAGTACGGAATCTGCTTGGTAACTACCTGCCGGAAGGTGAAACCTTGCCGGAAATTCCG
GATTCGATACCGACTATAGCTATTCCTACTATTAAAATGACTGAAATGGCAGTCTTATTT
GATAATCTGCCTCACCCCAAAGAGAGTGAGGATATCCGGACAATGGAAGCTTTTGATCAG
GGATGGGGTAGTATTCTGTATCGGACTTCATTATCCGCTTCTGATAAAGAACAGACATTA
CTGATCACAGAAGCTCATGACTGGGCACAAGTCTTTCTGAATGGTAAGAAACTGGCTACT
CTGAGCCGTTTGAAGGGGGAAGGTGTAGTAAAGTTACCACCATTGAAAGAAGGTGACAGA
CTGGATATCCTGGTGGAAGCAATGGGAAGAATGAATTTCGGCAAAGGTATTTATGACTGG
AAAGGTATTACCGAGAAAGTAGAACTGCAATCTGATAAAGGAGTTGAACTGGTGAAGGAC
TGGCAGGTATATACTATTCCGGTAGATTATAGTTTTGCCCGTGATAAGCAGTATAAACAA
CAGGAGAATGCTGAAAATCAACCGGCTTATTATCGGTCGACGTTTAACCTCAATGAGTTA
GGGGATACATTCTTGAATATGATGAACTGGAGTAAAGGTATGGTATGGGTGAATGGCCAT
GCGATTGGTCGTTATTGGGAAATCGGACCTCAACAGACCTTATATGTACCGGGCTGCTGG
TTGAAGAAAGGAGAGAATGAAATCATTATATTGGATATGGCTGGTCCTTCAAAAGCTGAA
ACTGAAGGTCTGCGTCAACCAATTCTGGATGTACAACGTGGTAACGGAGCTTATGCTCAT
CGTAAGATGGGTGAGAACTTGGACTTAACAAATGAAACTCCTGTTTATCAAGGGATATTT
AAGTCAGGCAATGGTTGGCAACATGTGTAG
Protein sequence predicted based on nucleotide sequence
SQSSEGTFEVGKNTFLLNGEPFVVKAAEIHYPRIPKEYWEHRIKMCKALGMNTICLYVFW NFHEPEEGRYDFAGQKDIAAFCRLAQENGMYVIVRPGPYVCAEWEMGGLPWWLLKKKDIK LREQDPYYMERVKLFLNEVGKQLADLQISKGGNIIMVQVENEYGAFGIDKPYISEIRDMV KQAGFTGVPLFQCDWNSNFENNALDDLLWTINFGTGANIDEQFKRLKELRPDTPLMCSEF WSGWFDHWGAKHETRSAEELVKGMKEMLDRNISFSLYMTHGGTSFGHWGGANFPNFSPTC TSYDYDAPINESGKVTPKYLEVRNLLGNYLPEGETLPEIPDSIPTIAIPTIKMTEMAVLF DNLPHPKESEDIRTMEAFDQGWGSILYRTSLSASDKEQTLLITEAHDWAQVFLNGKKLAT LSRLKGEGVVKLPPLKEGDRLDILVEAMGRMNFGKGIYDWKGITEKVELQSDKGVELVKD WQVYTIPVDYSFARDKQYKQQENAENQPAYYRSTFNLNELGDTFLNMMNWSKGMVWVNGH AIGRYWEIGPQQTLYVPGCWLKKGENEIIILDMAGPSKAETEGLRQPILDVQRGNGAYAH RKMGENLDLTNETPVYQGIFKSGNGWQHV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
