mll0582 (M. japonicum MAFF 303099 )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  mll0582
Gene Name:  hypothetical protein
Original Clone ID:
BGT25_T02a1
Keyword:
full-length CDS
Species:
Mesorhizobium japonicum MAFF 303099
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
for N-term fusion with a TEV cleavage site (no start codon)
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
MlCD00405652
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 2793nts
Open reading frame: 1 to 2793

AGCACCATCGGCCTGTGCATGATTGTCAAGAACGAGACGAAGGTCATCCTGCAATGCCTC
GCCAGCGTGCTCCCCCTTGTCGACTACGTGCTCATCGTCGACACCGGTTCCGAGGACGGC
ACGCAAGACCTGATACGCGGCTTTCTCGCCGATAACGGTGTCCAAGGCGCTGTCATTGAC
GAGCCATGGCGGGACTTCGCGTACAACCGGACCTTCGCCCTTGAACGATTGCGTAAAGTC
GAGACTGTCGACTATGCAATGATCATCGACGCGGACGATGTTCTGATCCGGGACGCCGAC
TTCGATCCGATCAGGTTCAAGTCGCAGATGGAACACGATTTATACGACGTCGAGGTGTTA
CATGGTGGCATTTCGTTTTATCGCCCGCAGATCTGCCGCAACCGTCTGCCGTTCGCCTTC
AAGGGCGTCCTGCACGAATATCTCGAGGCCCCTCCTGGCCATCTCACGCGGGAGACCGCC
AAGGGTTTTCGCGTGGCGACGGGGCGTGGCGGAGCACGCAGTCAAAACCCACGAAAGTAC
CAGGACGACTCGGCCGTTCTTGAAAATGCGCTTGCAACGGAGACCGACCCCTTTCTTGTT
TCGCGATACACCTTCTATCTGGCGCAGAGCTACAGGGATTCCGGCGAGCGTGAAAAATCA
CTCGCGAACTATCTGAAGCGCGCCGATCTAGGTTTTTGGGACGAGGAGGTCTATGTCAGC
CTGCTTGAGGCGGGCAACCTCATGGCAGCATTGGGGCGGCCATTCGACGAGGTCGTTGCC
GCCTTTGAGCGCGCGGCGCAGGTCGTCCCCGCCCGCGCGGAGGCTCTACACGCTGCGAGC
CTCTATTGCCGCAACCAGGGCCGGAATGCGGAAGGAACGGAATTTGCGCGTCGAGGGCTC
GACCTTGTTCAACCCGCCGGACTTTTCATTCAGCCCTGGGTTTACGATTACGGACTGCTC
GACGAGTTTGCTGTCAATGCCTACTGGGCAGGCGCCTACCGGGAATCGCTCGACGCTTGT
CTAAAATTGCTCGCCTCCGACAAATTGCCTGCGGAAATGGCGAAGCGTGTGGTGGCCAAC
GCTCGCTTCGCCACCGGCAAGCTTCCGGTCAATGAGCCGCCGAAACTTGGGGCGTTCGGG
ACCGAGAGCTTGATCGACCAGCACAGGCTTGTCCGGCAGCGGTCGTTGCGGTCGCGCATC
GAGGGCACGCCGCGCATCCTGGTGACAATCCTCGCAAAGCAGAAAGAGCCGGCACTGCCG
CTGTACCTCGAGTGCATCGAGGCGCTCGACTACCCCAAGGCGTCGATCGTCCTGTACATC
AGGACAAACAACAACACCGACAGGACCGAGCACATACTGCGCGAATGGGTGGAGCGCGTT
GGTCACCTCTATGCCGCGGTTGAATTCGATGCCTCCAACGTGGCCGATAGAGTCGAGCAG
TTTGGCGAACACGAATGGAACGAGACGCGCTTCAGGGTGCTTGGCCGAATCCGCAACATA
AGTTTGCGGAAAACTCTCGAACACAGCTGCGATTTCTATTTCGTTGCCGACGTCGATAAT
TTCGTGCGACCGGCCACGCTACGCGAGCTTGTCGCGCTCGACGTGCCGATCGTCGCTCCC
CTGCTTCGCTCCATCTCTCCCGGGCAATACTATTCGAACTACCACGCCGAAATCGACGCC
AACGGCTACTATATGCAGTGCGATCAATATGGGTGGGTGCTCAACCGCCACGTGCGCGGC
ATTATCGAGATGCCGCTTGTGCACTGCACCTATCTTGTCCGCGCCGACGTACTGCCCGAA
CTGACATACGAGGACGCGACATCGCGCTACGAGTACGTGATCTTTGCCGACAGCGCCCGC
AAGGCCGGCATCGTCCAGTATATGGATAACCGACAAGTCTATGGCTACATCACCTTCGGC
GATGGCCAATATTATGTGAGTGACGGGATCGAGCGCGCCCGAGCGCTGTTGCACGGTGCC
GGCGACACGCCGGTATTCACTGCCGCCACGCCTCCGGGCGCCGTACCGATGCCAGATCGA
CATGTCGAGCCGCCGCAAATTCACCTAATAAACTTGGACAGCAGCGTCGAGCGCTGGCGC
AAATATCAAGATAGGAATCCTCATCTAGCCGCCAATACAATTCGGGTATCGGCCGTCGAC
GGAGCATCCCTCGATAGGTCGACCTTGCAAGCCTTGGTGGACGATGGAGTGATTGCCGAG
GATTGCGGGTATCTGCCGGGCGCATTGGGATGTGCTCTATCGCACATCAACTTGTGGAAG
TTAGCGGTATCCGAGAAGCGGCCAATTACCATTTTCGAGGACGACGTCTACTCGTCGTTC
AACTTTCTTGAGGAGTCGACTCGCATTCTGTCCATGGCTCCAGAGAACTGGGATATGGTA
AAGTGGGGATTCAATTTCGACCCTCTATTCCTGTGGTTGAACTTCGATTTCTCCAAAGCC
AAGCTGGAGTTCTACAACCGACAACACAAACAGATTCCGGCCCAATTTCAATCTGAAACC
TATCCGCGATCTCTTATCCGACTAGTCCATTCCTTTGGTGCAATGGCTTATTCAGTAACT
CCAAGGGGAGCTCAAGTACTTCTTGAAAAATGCCTACCTCTTCGAAAGCGGCTTATTCCG
TTTCCTGGGACTGGAGTGGTCCTCGAGGATGAGGGCATCGACTGCGCAATGTCCGCGGTG
TATGACTCGATGCATGCGTTCGTCTGCATGCCGCCACTGGTCATTCACGACGACGAGCAG
GCATCAGATCGGGAAGTCGCGAATCGGCAGTAG

Protein sequence predicted based on nucleotide sequence

STIGLCMIVKNETKVILQCLASVLPLVDYVLIVDTGSEDGTQDLIRGFLADNGVQGAVID
EPWRDFAYNRTFALERLRKVETVDYAMIIDADDVLIRDADFDPIRFKSQMEHDLYDVEVL
HGGISFYRPQICRNRLPFAFKGVLHEYLEAPPGHLTRETAKGFRVATGRGGARSQNPRKY
QDDSAVLENALATETDPFLVSRYTFYLAQSYRDSGEREKSLANYLKRADLGFWDEEVYVS
LLEAGNLMAALGRPFDEVVAAFERAAQVVPARAEALHAASLYCRNQGRNAEGTEFARRGL
DLVQPAGLFIQPWVYDYGLLDEFAVNAYWAGAYRESLDACLKLLASDKLPAEMAKRVVAN
ARFATGKLPVNEPPKLGAFGTESLIDQHRLVRQRSLRSRIEGTPRILVTILAKQKEPALP
LYLECIEALDYPKASIVLYIRTNNNTDRTEHILREWVERVGHLYAAVEFDASNVADRVEQ
FGEHEWNETRFRVLGRIRNISLRKTLEHSCDFYFVADVDNFVRPATLRELVALDVPIVAP
LLRSISPGQYYSNYHAEIDANGYYMQCDQYGWVLNRHVRGIIEMPLVHCTYLVRADVLPE
LTYEDATSRYEYVIFADSARKAGIVQYMDNRQVYGYITFGDGQYYVSDGIERARALLHGA
GDTPVFTAATPPGAVPMPDRHVEPPQIHLINLDSSVERWRKYQDRNPHLAANTIRVSAVD
GASLDRSTLQALVDDGVIAEDCGYLPGALGCALSHINLWKLAVSEKRPITIFEDDVYSSF
NFLEESTRILSMAPENWDMVKWGFNFDPLFLWLNFDFSKAKLEFYNRQHKQIPAQFQSET
YPRSLIRLVHSFGAMAYSVTPRGAQVLLEKCLPLRKRLIPFPGTGVVLEDEGIDCAMSAV
YDSMHAFVCMPPLVIHDDEQASDREVANRQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable