Gene:
Gene Symbol:
BIVM-ERCC5
Gene Name:
BIVM-ERCC5 readthrough
Original Clone ID:
FLH180237.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00040780
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGGGTCCAGGGGCTCTGGAAGCTGCTGGAGTGCTCCGGGCGGCAGGTCAGCCCCGAA
GCGCTGGAAGGGAAGATCCTGGCTGTTGATATTAGCATTTGGTTAAACCAAGCACTTAAA
GGAGTCCGGGATCGCCACGGGAACTCAATAGAAAATCCTCATCTTCTCACTTTGTTTCAT
CGGCTCTGCAAACTCTTATTTTTTCGAATTCGTCCTATTTTTGTGTTTGATGGGGATGCT
CCACTATTGAAGAAACAGACTTTGGTGAAGAGAAGGCAGAGAAAGGACTTAGCGTCCAGT
GACTCCAGGAAAACGACAGAGAAGCTTCTGAAAACATTTTTGAAAAGACAAGCCATCAAA
ACTGCCTTCAGAAGCAAAAGAGATGAAGCACTACCCAGTCTTACCCAAGTTCGAAGAGAA
AACGACCTCTATGTTTTGCCTCCTTTACAAGAGGAAGAAAAACACAGTTCAGAAGAGGAA
GATGAAAAAGAATGGCAAGAAAGAATGAATCAAAAACAAGCATTACAGGAAGAGTTCTTT
CATAATCCTCAAGCGATAGATATTGAGTCTGAGGACTTCAGCAGCCTGCCCCCTGAAGTA
AAGCATGAAATCTTGACTGATATGAAAGAGTTCACCAAGCGCAGAAGAACATTATTTGAA
GCAATGCCAGAGGAGTCTGATGACTTTTCACAGTACCAACTCAAAGGCTTGCTTAAAAAG
AACTATCTGAACCAGCATATAGAACATGTCCAAAAGGAAATGAATCAGCAACATTCAGGA
CACATCCGAAGGCAGTATGAAGATGAAGGGGGCTTTCTGAAGGAGGTAGAGTCAAGGAGA
GTGGTCTCTGAAGACACTTCACATTACATCTTGATAAAAGGTATTCAAGCTAAGACAGTT
GCAGAAGTGGATTCAGAGTCTCTTCCTTCTTCCAGCAAAATGCACGGCATGTCTTTTGAC
GTGAAGTCATCTCCATGTGAAAAACTGAAGACAGAGAAAGAGCCTGATGCTACCCCTCCT
TCTCCAAGAACTTTACTAGCTATGCAAGCTGCCCTGCTGGGAAGTAGCTCAGAAGAGGAG
CTGGAGAGTGAAAATCGAAGGCAGGCCCGTGGGAGGAACGCACCTGCTGCTGTAGACGAA
GGCTCCATATCACCCCGGACTCTTTCAGCCATTAAGAGAGCTCTTGACGATGACGAAGAT
GTAAAAGTGTGTGCTGGGGATGATGTGCAGACGGGAGGGCCAGGAGCAGAAGAAATGCGT
ATAAACAGCTCCACCGAGAACAGTGATGAAGGACTTAAAGTGAGAGATGGAAAAGGAATA
CCGTTTACTGCAACACTTGCGTCATCTAGTGTGAACTCTGCAGAGGAGCACGTAGCCAGC
ACTAATGAGGGGAGAGAGCCCACAGACTCAGTTCCAAAAGAACAAATGTCACTTGTTCAC
GTGGGGACTGAAGCCTTTCCGATAAGTGATGAGTCTATGATTAAGGACAGAAAAGATCGG
CTGCCTCTGGAGAGTGCAGTGGTTAGACATAGTGACGCACCTGGGCTCCCGAATGGAAGG
GAACTGACACCGGCATCTCCAACTTGTACAAATTCTGTGTCAAAGAATGAAACACATGCT
GAAGTGCTTGAGCAGCAGAACGAACTTTGCCCATATGAGAGTAAATTCGATTCTTCTCTT
CTTTCAAGTGATGATGAAACAAAATGTAAACCGAATTCTGCTTCTGAAGTCATTGGCCCT
GTCAGTTTGCAAGAAACAAGTAGCATAGTAAGTGTCCCTTCAGAGGCAGTAGATAATGTG
GAAAATGTGGTGTCATTTAATGCTAAAGAGCATGAGAATTTTCTGGAAACCATCCAAGAA
CAGCAGACCACTGAATCTGCAGGCCAGGATTTAATTTCCATTCCAAAGGCCGTGGAACCA
ATGGAAATTGACTCGGAAGAAAGTGAATCTGATGGAAGTTTCATTGAAGTGCAAAGTGTG
ATTAGTGATGAGGAACTTCAAGCAGAATTCCCTGAAACTTCCAAACCTCCCTCAGAACAA
GGCGAAGAGGAACTGGTAGGAACTAGGGAGGGAGAAGCCCCTGCTGAGTCCGAGAGCCTC
CTGAGGGACAACTCTGAGAGGGACGACGTGGATGGTGAGCCACAGGAAGCTGAGAAAGAT
GCGGAAGATTCGCTCCATGAATGGCAAGATATTAATTTGGAGGAGTTGGAAACTCTGGAG
AGCAACCTCTTAGCACAGCAGAATTCACTGAAAGCTCAAAAACAGCAGCAAGAACGGATC
GCTGCTACTGTCACCGGACAGATGTTCCTGGAAAGCCAGGAACTCCTGCGCCTGTTCGGC
ATTCCCTACATCCAGGCTCCCATGGAAGCAGAGGCGCAGTGCGCCATCCTGGACCTGACT
GATCAGACTTCCGGAACCATCACTGATGACAGTGATATCTGGCTGTTTGGAGCGCGGCAT
GTCTATAGAAACTTTTTTAATAAAAACAAGTTTGTAGAATATTATCAATATGTGGACTTT
CACAATCAATTGGGATTGGACCGGAATAAGTTAATAAATTTGGCTTATTTGCTTGGAAGT
GATTATACCGAAGGAATACCAACTGTGGGTTGTGTAACCGCCATGGAAATTCTCAATGAA
TTCCCTGGGCATGGCCTGGAACCTCTCCTAAAATTCTCAGAATGGTGGCATGAAGCTCAA
AAAAATCCAAAGATAAGACCTAATCCTCATGACACCAAAGTGAAAAAAAAATTACGGACA
TTGCAACTCACCCCTGGCTTTCCTAACCCAGCTGTTGCCGAGGCCTACCTCAAACCCGTG
GTGGATGACTCGAAGGGATCCTTTCTGTGGGGGAAACCTGATCTCGACAAAATTAGAGAA
TTTTGTCAGCGGTATTTCGGCTGGAACAGAACGAAGACAGATGAATCTCTGTTTCCTGTA
TTAAAGCAACTCGATGCCCAGCAGACACAGCTCCGAATTGATTCCTTCTTTAGATTAGCA
CAACAGGAGAAAGAAGATGCTAAACGTATTAAGAGCCAGAGACTAAACAGAGCTGTGACA
TGTATGCTAAGGAAAGAGAAAGAAGCAGCAGCCAGCGAAATAGAAGCAGTTTCTGTTGCC
ATGGAGAAAGAATTTGAGCTACTTGATAAGGCAAAACGAAAAACCCAGAAGAGAGGCATA
ACAAATACCTTAGAAGAGTCATCAAGCCTGAAAAGAAAGAGGCTTTCAGATTCTAAACGA
AAGAATACATGCGGTGGATTTTTGGGGGAGACCTGCCTCTCAGAATCATCTGATGGATCT
TCAAGTGAAGATGCTGAAAGTTCATCTTTAATGAATGTACAAAGGAGAACAGCTGCGAAA
GAGCCAAAAACCAGTGCTTCAGATTCGCAGAACTCAGTGAAGGAAGCTCCCGTGAAGAAT
GGAGGTGCGACCACCAGCAGCTCTAGTGATAGTGATGACGATGGAGGGAAAGAGAAGATG
GTCCTCGTGACCGCCAGATCTGTGTTTGGGAAGAAAAGAAGGAAACTAAGACGTGCGAGG
GGAAGAAAAAGGAAAACC
Protein sequence predicted based on nucleotide sequence
MGVQGLWKLLECSGRQVSPEALEGKILAVDISIWLNQALKGVRDRHGNSIENPHLLTLFH RLCKLLFFRIRPIFVFDGDAPLLKKQTLVKRRQRKDLASSDSRKTTEKLLKTFLKRQAIK TAFRSKRDEALPSLTQVRRENDLYVLPPLQEEEKHSSEEEDEKEWQERMNQKQALQEEFF HNPQAIDIESEDFSSLPPEVKHEILTDMKEFTKRRRTLFEAMPEESDDFSQYQLKGLLKK NYLNQHIEHVQKEMNQQHSGHIRRQYEDEGGFLKEVESRRVVSEDTSHYILIKGIQAKTV AEVDSESLPSSSKMHGMSFDVKSSPCEKLKTEKEPDATPPSPRTLLAMQAALLGSSSEEE LESENRRQARGRNAPAAVDEGSISPRTLSAIKRALDDDEDVKVCAGDDVQTGGPGAEEMR INSSTENSDEGLKVRDGKGIPFTATLASSSVNSAEEHVASTNEGREPTDSVPKEQMSLVH VGTEAFPISDESMIKDRKDRLPLESAVVRHSDAPGLPNGRELTPASPTCTNSVSKNETHA EVLEQQNELCPYESKFDSSLLSSDDETKCKPNSASEVIGPVSLQETSSIVSVPSEAVDNV ENVVSFNAKEHENFLETIQEQQTTESAGQDLISIPKAVEPMEIDSEESESDGSFIEVQSV ISDEELQAEFPETSKPPSEQGEEELVGTREGEAPAESESLLRDNSERDDVDGEPQEAEKD AEDSLHEWQDINLEELETLESNLLAQQNSLKAQKQQQERIAATVTGQMFLESQELLRLFG IPYIQAPMEAEAQCAILDLTDQTSGTITDDSDIWLFGARHVYRNFFNKNKFVEYYQYVDF HNQLGLDRNKLINLAYLLGSDYTEGIPTVGCVTAMEILNEFPGHGLEPLLKFSEWWHEAQ KNPKIRPNPHDTKVKKKLRTLQLTPGFPNPAVAEAYLKPVVDDSKGSFLWGKPDLDKIRE FCQRYFGWNRTKTDESLFPVLKQLDAQQTQLRIDSFFRLAQQEKEDAKRIKSQRLNRAVT CMLRKEKEAAASEIEAVSVAMEKEFELLDKAKRKTQKRGITNTLEESSSLKRKRLSDSKR KNTCGGFLGETCLSESSDGSSSEDAESSSLMNVQRRTAAKEPKTSASDSQNSVKEAPVKN GGATTSSSSDSDDDGGKEKMVLVTARSVFGKKRRKLRRARGRKRKT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3973
Start on reference sequence
413
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
