MAN2B1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MAN2B1
Gene Name:  mannosidase, alpha, class 2B, member 1
Original Clone ID:
FLH180864.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00040828
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3033nts
Open reading frame: 1 to 3033

ATGGGCGCCTACGCGCGGGCTTCGGGGGTCTGCGCTCGCGGCTGCCTGGACTCAGCAGGC
CCCTGGACCATGTCCCGCGCCCTGCGGCCACCGCTCCCGCCTCTCTGCTTTTTCCTTTTG
TTGCTGGCGGCTGCCGGTGCTCGGGCCGGGGGATACGAGACATGCCCCACAGTGCAGCCG
AACATGCTGAACGTGCACCTGCTGCCTCACACACATGATGACGTGGGCTGGCTCAAAACC
GTGGACCAGTACTTTTATGGAATCAAGAATGACATCCAGCACGCCGGTGTGCAGTACATC
CTGGACTCGGTCATCTCTGCCTTGCTGGCAGATCCCACCCGTCGCTTCATTTACGTGGAG
ATTGCCTTCTTCTCCCGTTGGTGGCACCAGCAGACAAATGCCACACAGGAAGTCGTGCGA
GACCTTGTGCGCCAGGGGCGCCTGGAGTTCGCCAATGGTGGCTGGGTGATGAACGATGAG
GCAGCCACCCACTACGGTGCCATCGTGGACCAGATGACACTTGGGCTGCGCTTTCTGGAG
GACACATTTGGCAATGATGGGCGACCCCGTGTGGCCTGGCACATTGACCCCTTCGGCCAC
TCTCGGGAGCAGGCCTCGCTGTTTGCGCAGATGGGCTTCGACGGCTTCTTCTTTGGGCGC
CTTGATTATCAAGATAAGTGGGTACGGATGCAGAAGCTGGAGATGGAGCAGGTGTGGCGG
GCCAGCACCAGCCTGAAGCCCCCGACCGCGGACCTCTTCACTGGTGTGCTTCCCAATGGT
TACAACCCGCCAAGGAATCTGTGCTGGGATGTGCTGTGTGTCGATCAGCCGCTGGTGGAG
GACCCTCGCAGCCCCGAGTACAACGCCAAGGAGCTGGTCGATTACTTCCTAAATGTGGCC
ACTGCCCAGGGCCGGTATTACCGCACCAACCACACTGTGATGACCATGGGCTCGGACTTC
CAATATGAGAATGCCAACATGTGGTTCAAGAACCTTGACAAGCTCATCCGGCTGGTAAAT
GCGCAGCAGGCAAAAGGAAGCAGTGTCCATGTTCTCTACTCCACCCCCGCTTGTTACCTC
TGGGAGCTGAACAAGGCCAACCTCACCTGGTCAGTGAAACATGACGACTTCTTCCCTTAC
GCGGATGGCCCCCACCAGTTCTGGACCGGTTACTTTTCCAGTCGGCCGGCCCTCAAACGC
TACGAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAACCAGCTGGAGGCGCTGGTGGGC
CTGGCGGCCAACGTGGGACCCTATGGCTCCGGAGACAGTGCACCCCTCAATGAGGCGATG
GCTGTGCTCCAGCATCACGACGCCGTCAGCGGCACCTCCCGCCAGCACGTGGCCAACGAC
TACGCGCGCCAGCTTGCGGCAGGCTGGGGGCCTTGCGAGGTTCTTCTGAGCAACGCGCTG
GCGCGGCTCAGAGGCTTCAAAGATCACTTCACCTTTTGCCAACAGCTAAACATCAGCATC
TGCCCGCTCAGCCAGACGGCGGCGCGCTTCCAGGTCATCGTTTATAATCCCCTGGGGCGG
AAGGTGAATTGGATGGTACGGCTGCCGGTCAGCGAAGGCGTTTTCGTTGTGAAGGACCCC
AATGGCAGGACAGTGCCCAGCGATGTGGTAATATTTCCCAGCTCAGACAGCCAGGCGCAC
CCTCCGGAGCTGCTGTTCTCAGCCTCACTGCCCGCCCTGGGCTTCAGCACCTATTCAGTA
GCCCAGGTGCCTCGCTGGAAGCCCCAGGCCCGCGCACCACAGCCCATCCCCAGAAGATCC
TGGTCCCCTGCTTTAACCATCGAAAATGAGCACATCCGGGCAACGTTTGATCCTGACACA
GGGCTGTTGATGGAGATTATGAACATGAATCAGCAACTCCTGCTGCCTGTTCGCCAGACC
TTCTTCTGGTACAACGCCAGTATAGGTGACAACGAAAGTGACCAGGCCTCAGGTGCCTAC
ATCTTCAGACCCAACCAACAGAAACCGCTGCCTGTGAGCCGCTGGGCTCAGATCCACCTG
GTGAAGACACCCTTGGTGCAGGAGGTGCACCAGAACTTCTCAGCTTGGTGTTCCCAGGTG
GTTCGCCTGTACCCAGGACAGCGGCACCTGGAGCTAGAGTGGTCGGTGGGGCCGATACCT
GTGGGCGACACCTGGGGGAAGGAGGTCATCAGCCGTTTTGACACACCGCTGGAGACAAAG
GGACGCTTCTACACAGACAGCAATGGCCGGGAGATCCTGGAGAGGAGGCGGGATTATCGA
CCCACCTGGAAACTGAACCAGACGGAGCCCGTGGCAGGAAACTACTATCCAGTCAACACC
CGGATTTACATCACGGATGGAAACATGCAGCTGACTGTGCTGACTGACCGCTCCCAGGGG
GGCAGCAGCCTGAGAGATGGCTCGCTGGAGCTCATGGTGCACCGAAGGCTGCTGAAGGAC
GATGGACGCGGAGTATCGGAGCCACTAATGGAGAACGGGTCGGGGGCGTGGGTGCGAGGG
CGCCACCTGGTGCTGCTGGACACAGCCCAGGCTGCAGCCGCCGGACACCGGCTCCTGGCG
GAGCAGGAGGTCCTGGCCCCTCAGGTGGTGCTGGCCCCGGGTGGCGGCGCCGCCTACAAT
CTCGGGGCTCCTCCGCGCACGCAGTTCTCAGGGCTGCGCAGGGACCTGCCGCCCTCGGTG
CACCTGCTCACGCTGGCCAGCTGGGGCCCCGAAATGGTGCTGCTGCGCTTGGAGCACCAG
TTTGCCGTAGGAGAGGATTCCGGACGTAACCTGAGCGCCCCCGTTACCTTGAACTTGAGG
GACCTGTTCTCCACCTTCACCATCACCCGCCTGCAGGAGACCACGCTGGTGGCCAACCAG
CTCCGCGAGGCAGCCTCCAGGCTCAAGTGGACAACAAACACAGGCCCCACACCCCACCAA
ACTCCGTACCAGCTGGACCCGGCCAACATCACGCTGGAACCCATGGAAATCCGCACTTTC
CTGGCCTCAGTTCAATGGAAGGAGGTGGATGGT

Protein sequence predicted based on nucleotide sequence

MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQP
NMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVE
IAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLE
DTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWR
ASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVA
TAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVHVLYSTPACYL
WELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVG
LAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNAL
ARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDP
NGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRS
WSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAY
IFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIP
VGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNT
RIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRG
RHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSV
HLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQ
LREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 3067
Start on reference sequence 32
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable