MCF2L (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MCF2L
Gene Name:  MCF.2 cell line derived transforming sequence-like
Original Clone ID:
FLH182595.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
HsCD00040995
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2952nts
Open reading frame: 1 to 2952

ATGACGGTGCGCCGGCTGTCACTGCTGTGCCGGGACCTCTGGGCGCTGTGGCTGCTGCTG
AAGGCCGGCGCAGATGAAATCATGCACCAGGACATCGTCCCGCTCTGTGCTGCCGACATC
CAGGACCAGCTAAAGAAGCGCTTTGCTTACCTGTCCGGTGGGCGGGGGCAGGACGGAAGC
CCGGTTATCACCTTCCCTGACTACCCGGCCTTCAGCGAGATTCCGGACAAGGAGTTCCAG
AATGTCATGACCTACCTCACCAGCATCCCCAGCCTGCAGGACGCTGGCATCGGATTCATC
CTGGTGATAGACCGGCGACGGGACAAATGGACCTCCGTGAAGGCGTCCGTCCTGCGCATC
GCAGCATCTTTCCCGGCAAACCTGCAGCTCGTCCTCGTGCTTCGCCCGACGGGTTTTTTC
CAAAGGACTCTCTCCGACATCGCTTTCAAATTCAATAGAGATGACTTTAAGATGAAGGTG
CCGGTCATAATGCTGAGCTCCGTACCAGACTTACACGGTTACATCGATAAGTCGCAGCTG
ACCGAGGACCTGGGTGGGACCCTGGACTACTGCCACTCCCGGTGGCTGTGCCAGCGCACG
GCCATCGAAAGTTTCGCCCTCATGGTGAAGCAGACGGCTCAGATGCTGCAGTCCTTCGGG
ACCGAGCTGGCTGAAACAGAGCTGCCCAATGACGTCCAGTCGACAAGCTCAGTGCTGTGT
GCGCACACAGAGAAGAAGGACAAGGCGAAGGAGGATTTGAGGCTGGCACTGAAAGAGGGG
CACAGTGTCCTGGAGAGCCTCAGGGAGCTGCAGGCTGAGGGCTCAGAGCCCAGTGTGAAC
CAGGACCAGCTTGACAACCAGGCCACCGTGCAGAGGCTCCTGGCCCAGCTGAACGAAACC
GAGGCTGCCTTCGATGAGTTCTGGGCAAAGCATCAGCAGAAACTGGAGCAGTGTCTGCAG
CTCCGGCACTTTGAGCAGGGCTTCCGGGAGGTCAAAGCCATCTTGGACGCAGCGTCCCAG
AAGATAGCAACCTTCACAGACATCGGCAACAGCCTGGCGCATGTGGAGCACCTGCTGAGG
GACCTGGCCAGCTTCGAGGAGAAATCAGGCGTGGCCGTGGAGAGGGCCCGGGCCCTGTCT
CTGGACGGCGAGCAGCTCATTGGGAACAAGCACTACGCGGTAGACTCCATCCGCCCAAAG
TGCCAGGAGCTCCGGCACCTCTGTGACCAGTTCTCTGCGGAGATCGCAAGGAGGAGGGGG
CTGCTCAGCAAGTCCCTGGAGCTGCACCGCCGCCTGGAGACGTCCATGAAGTGGTGTGAT
GAAGGGATTTACCTGCTGGCCTCACAACCTGTGGACAAGTGCCAGTCCCAGGACGGCGCG
GAGGCTGCCCTCCAGGAAATCGAGAAGTTTTTGGAGACCGGTGCGGAAAATAAGATCCAG
GAGCTCAACGCGATTTACAAGGAATACGAATCCATCCTCAACCAAGATCTCATGGAGCAC
GTGCGAAAGGTCTTCCAGAAGCAGGCAAGCATGGAGGAGGTGTTCCACCGCAGGCAGGCC
AGCCTGAAGAAGCTGGCGGCCAGGCAGACGCGGCCCGTGCAGCCGGTGGCCCCCAGACCC
GAGGCACTGGCAAAGTCGCCCTGCCCCTCCCCAGGCATTCGGCGAGGCTCTGAGAACTCC
AGCTCCGAGGGCGGTGCGCTCCGGAGAGGGCCCTACCGGAGGGCCAAGAGTGAGATGAGT
GAGAGCCGGCAGGGCCGCGGCTCAGCGGGGGAGGAGGAGGAAAGCCTGGCCATCCTGCGC
AGGCACGTGATGAGCGAGCTCCTGGACACAGAACGGGCCTACGTGGAGGAGCTGCTGTGC
GTCCTGGAGGGCTACGCCGCGGAGATGGATAACCCACTGATGGCTCACCTCCTGTCAACA
GGCCTTCACAACAAGAAGGATGTTTTGTTTGGAAACATGGAGGAAATCTATCACTTCCAC
AACAGGATATTCCTCAGGGAGCTGGAAAACTACACTGACTGCCCAGAACTGGTTGGAAGA
TGCTTTCTGGAGAGGATGGAAGATTTCCAGATCTATGAGAAGTACTGTCAGAACAAGCCC
CGCTCTGAGAGCCTGTGGAGACAGTGCTCCGACTGCCCGTTTTTCCAGGAATGCCAGAGA
AAGCTGGACCACAAGCTGAGCCTGGACTCCTACCTGCTGAAGCCAGTGCAGAGGATCACC
AAGTACCAGCTGCTGCTCAAGGAAATGCTGAAATACAGCAGGAACTGCGAGGGGGCTGAG
GACCTGCAGGAGGCGCTGAGCTCCATCCTGGGCATCCTGAAGGCCGTGAACGACTCCATG
CACCTCATCGCTATCACCGGCTATGACGGGAATCTCGGCGACCTGGGCAAGCTGCTGATG
CAGGGCTCGTTCAGCGTCTGGACCGACCACAAGAGGGGCCACACCAAGGTGAAGGAGCTG
GCCAGGTTCAAGCCCATGCAGCGGCACCTGTTCCTGCACGAGAAGGCAGTGCTCTTCTGC
AAGAAGAGGGAGGAGAATGGGGAGGGGTATGAGAAAGCTCCCTCCTACAGCTACAAGCAG
TCCTTAAACATGGCTGCCGTTGGCATTACGGAGAACGTGAAGGGAGATGCTAAGAAGTTC
GAGATCTGGTACAACGCGCGCGAGGAGGTCTACATCGTCCAGGCGCCAACTCCTGAGATT
AAAGCCGCGTGGGTGAATGAAATTCGGAAAGTGCTGACCAGCCAGCTGCAGGCTTGTAGA
GAAGCCAGCCAGCACCGGGCGCTGGAGCAGTCACAGAGCCTGCCCCTGCCGGCCCCGACC
AGCACCAGTCCCTCAAGAGGAAACTCAAGGAACATCAAGAAGCTGGAAGAAAGGAAAACA
GACCCCCTAAGCCTGGAGGGATACGTCAGCTCAGCGCCACTGACAAAGCCCCCCGAAAAG
GGCAAAGAGCCC

Protein sequence predicted based on nucleotide sequence

MTVRRLSLLCRDLWALWLLLKAGADEIMHQDIVPLCAADIQDQLKKRFAYLSGGRGQDGS
PVITFPDYPAFSEIPDKEFQNVMTYLTSIPSLQDAGIGFILVIDRRRDKWTSVKASVLRI
AASFPANLQLVLVLRPTGFFQRTLSDIAFKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQL
TEDLGGTLDYCHSRWLCQRTAIESFALMVKQTAQMLQSFGTELAETELPNDVQSTSSVLC
AHTEKKDKAKEDLRLALKEGHSVLESLRELQAEGSEPSVNQDQLDNQATVQRLLAQLNET
EAAFDEFWAKHQQKLEQCLQLRHFEQGFREVKAILDAASQKIATFTDIGNSLAHVEHLLR
DLASFEEKSGVAVERARALSLDGEQLIGNKHYAVDSIRPKCQELRHLCDQFSAEIARRRG
LLSKSLELHRRLETSMKWCDEGIYLLASQPVDKCQSQDGAEAALQEIEKFLETGAENKIQ
ELNAIYKEYESILNQDLMEHVRKVFQKQASMEEVFHRRQASLKKLAARQTRPVQPVAPRP
EALAKSPCPSPGIRRGSENSSSEGGALRRGPYRRAKSEMSESRQGRGSAGEEEESLAILR
RHVMSELLDTERAYVEELLCVLEGYAAEMDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFH
NRIFLRELENYTDCPELVGRCFLERMEDFQIYEKYCQNKPRSESLWRQCSDCPFFQECQR
KLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSRNCEGAEDLQEALSSILGILKAVNDSM
HLIAITGYDGNLGDLGKLLMQGSFSVWTDHKRGHTKVKELARFKPMQRHLFLHEKAVLFC
KKREENGEGYEKAPSYSYKQSLNMAAVGITENVKGDAKKFEIWYNAREEVYIVQAPTPEI
KAAWVNEIRKVLTSQLQACREASQHRALEQSQSLPLPAPTSTSPSRGNSRNIKKLEERKT
DPLSLEGYVSSAPLTKPPEKGKEP

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 158
End on reference sequence 3112
Mutations:
None
Discrepancy:
a2402g,Q801R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable