DDX20 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DDX20
Gene Name:  DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
Original Clone ID:
FLH184505.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00041199
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2472nts
Open reading frame: 1 to 2472

ATGGCGGCGGCATTTGAAGCCTCGGGAGCCTTAGCGGCAGTGGCGACTGCTATGCCGGCT
GAGCATGTGGCCGTGCAGGTCCCGGCCCCAGAGCCAACACCCGGGCCTGTGAGGATCCTG
CGGACCGCTCAGGATCTCAGCAGCCCGCGGACCCGCACGGGGGATGTGCTGTTGGCGGAG
CCGGCCGACTTCGAGTCACTGCTGCTTTCGCGGCCGGTGCTGGAGGGGCTGCGGGCGGCC
GGCTTCGAGAGGCCCTCGCCGGTGCAGCTCAAGGCCATCCCGCTGGGGCGCTGCGGGCTC
GATTTAATTGTTCAAGCTAAATCTGGCACCGGGAAAACCTGTGTGTTCTCCACCATAGCT
TTGGACTCTCTTGTTCTTGAAAACTTAAGTACCCAGATTTTGATCTTGGCTCCTACAAGA
GAAATTGCTGTACAGATACATTCTGTTATTACAGCCATTGGAATAAAAATGGAAGGCTTA
GAGTGTCATGTCTTTATTGGAGGGACCCCATTATCACAAGACAAAACCAGACTTAAAAAG
TGTCATATTGCTGTTGGATCTCCTGGCAGAATTAAGCAACTCATAGAACTTGACTACTTG
AACCCAGGCAGTATACGCCTCTTTATTCTTGATGAAGCAGATAAGCTTTTAGAAGAAGGC
AGCTTCCAGGAGCAAATAAATTGGATTTATTCTTCCTTGCCTGCCAGTAAACAGATGCTG
GCAGTATCAGCTACTTATCCCGAATTTTTGGCTAATGCTTTGACAAAGTACATGAGAGAT
CCCACTTTTGTAAGACTGAATTCCAGTGATCCAAGTCTCATAGGTTTGAAGCAGTATTAC
AAAGTTGTCAATTCATACCCTTTGGCACATAAGGTTTTTGAGGAAAAGACTCAGCATTTA
CAGGAACTGTTCAGCAGAATTCCATTTAATCAAGCTTTAGTCTTTTCTAATTTGCACAGC
AGAGCACAACATTTGGCTGATATCCTTTCTTCTAAAGGCTTTCCTGCTGAGTGCATTTCA
GGCAATATGAATCAGAATCAGCGTCTTGATGCTATGGCTAAACTGAAGCACTTTCATTGC
AGAGTCCTCATTTCCACAGATTTGACTTCTCGTGGGATTGATGCTGAGAAGGTGAATCTG
GTTGTAAATCTGGATGTACCATTGGATTGGGAGACATACATGCATCGGATTGGGAGAGCT
GGCCGTTTTGGTACATTGGGGCTGACAGTGACCTACTGTTGCCGGGGAGAGGAAGAAAAT
ATGATGATGAGAATTGCCCAGAAATGTAATATCAACCTTCTCCCTTTACCAGATCCCATT
CCTTCTGGTCTGATGGAAGAATGTGTGGATTGGGATGTGGAAGTTAAAGCTGCTGTGCAT
ACATATGGTATAGCAAGTGTACCTAACCAACCCTTAAAAAAGCAAATTCAGAAAATAGAG
AGAACCCTTCAAATTCAGAAAGCTCATGGTGACCACATGGCTTCCTCTAGAAATAATTCT
GTATCTGGACTATCAGTCAAATCAAAAAATAATACCAAACAAAAGCTTCCTGTGAAAAGC
CACTCAGAATGTGGAATCATAGAAAAAGCAACATCACCAAAAGAACTGGGCTGTGACAGG
CAATCCGAAGAGCAAATGAAGAATTCTGTTCAGACTCCCGTTGAAAACTCCACCAACAGT
CAGCACCAGGTCAAAGAAGCTTTACCTGTGTCACTCCCCCAGATTCCTTGTCTGTCTTCC
TTTAAAATCCATCAGCCATACACGTTGACTTTTGCTGAATTGGTAGAGGATTATGAACAT
TATATTAAAGAGGGGTTAGAGAAACCTGTGGAAATCATCAGGCACTACACAGGCCCTGGG
GATCAGACTGTGAATCCTCAAAATGGTTTTGTGAGAAATAAAGTTACTGAACAGAGAGTC
CCTGTATTGGCAAGTAGTAGCCAATCTGGAGACTCTGAGAGTGACAGTGATTCTTACAGC
TCAAGAACCTCTTCCCAGAGCAAAGGAAATAAGTCATACTTGGAAGGCTCTTCTGATAAT
CAGCTGAAAGACTCTGAATCTACGCCTGTGGATGATCGTATTTCTTTGGAACAACCACCA
AATGGAAGTGACACCCCCAATCCAGAGAAATATCAAGAATCACCTGGAATCCAGATGAAG
ACAAGACTTAAAGAGGGGGCTAGCCAGAGAGCTAAGCAGAGCCGGAGAAACCTACCCAGG
CGGTCTTCCTTCAGATTGCAGACTGAAGCCCAGGAAGATGATTGGTATGACTGTCATAGG
GAAATACGTCTGAGTTTTTCTGATACCTATCAGGATTATGAGGAGTACTGGAGAGCTTAC
TACAGGGCATGGCAAGAATATTATGCTGCCGCTTCTCATTCATATTATTGGAATGCTCAG
AGACATCCAAGTTGGATGGCAGCTTATCACATGAATACCATTTATCTACAAGAAATGATG
CATAGTAACCAG

Protein sequence predicted based on nucleotide sequence

MAAAFEASGALAAVATAMPAEHVAVQVPAPEPTPGPVRILRTAQDLSSPRTRTGDVLLAE
PADFESLLLSRPVLEGLRAAGFERPSPVQLKAIPLGRCGLDLIVQAKSGTGKTCVFSTIA
LDSLVLENLSTQILILAPTREIAVQIHSVITAIGIKMEGLECHVFIGGTPLSQDKTRLKK
CHIAVGSPGRIKQLIELDYLNPGSIRLFILDEADKLLEEGSFQEQINWIYSSLPASKQML
AVSATYPEFLANALTKYMRDPTFVRLNSSDPSLIGLKQYYKVVNSYPLAHKVFEEKTQHL
QELFSRIPFNQALVFSNLHSRAQHLADILSSKGFPAECISGNMNQNQRLDAMAKLKHFHC
RVLISTDLTSRGIDAEKVNLVVNLDVPLDWETYMHRIGRAGRFGTLGLTVTYCCRGEEEN
MMMRIAQKCNINLLPLPDPIPSGLMEECVDWDVEVKAAVHTYGIASVPNQPLKKQIQKIE
RTLQIQKAHGDHMASSRNNSVSGLSVKSKNNTKQKLPVKSHSECGIIEKATSPKELGCDR
QSEEQMKNSVQTPVENSTNSQHQVKEALPVSLPQIPCLSSFKIHQPYTLTFAELVEDYEH
YIKEGLEKPVEIIRHYTGPGDQTVNPQNGFVRNKVTEQRVPVLASSSQSGDSESDSDSYS
SRTSSQSKGNKSYLEGSSDNQLKDSESTPVDDRISLEQPPNGSDTPNPEKYQESPGIQMK
TRLKEGASQRAKQSRRNLPRRSSFRLQTEAQEDDWYDCHREIRLSFSDTYQDYEEYWRAY
YRAWQEYYAAASHSYYWNAQRHPSWMAAYHMNTIYLQEMMHSNQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 11
End on reference sequence 2485
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable