DNASU Plasmid | ALG1 (Homo sapiens) in pGEn2-DEST

Explanation of Terms

Gene:

Gene Symbol: ALG1

Gene Name: ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase

Original Clone ID:

HGT33_M01

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pGEn2-DEST

Format:

FUSION

Source:

Glyco-enzyme clone collection

Description:

All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen

HsCD00413048

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 1323nts

Open reading frame: 1 to 1323

Reference Sequence Alignment

AAGCGCTGGCGCCGGGGGCGGGCGGCCCGGCATGTAGTAGCGGTGGTGCTGGGCGACGTG
GGCCGCAGCCCCCGTATGCAGTACCACGCGCTGTCGTTGGCCATGCACGGCTTCTCGGTG
ACCCTCCTGGGGTTCTGCAACTCCAAACCCCATGATGAGCTCTTGCAGAACAACAGAATT
CAGATTGTGGGGTTGACAGAACTTCAGAGTCTTGCAGTTGGGCCCCGAGTTTTCCAGTAC
GGAGTCAAAGTTGTACTTCAGGCTATGTACTTGCTGTGGAAGTTGATGTGGAGGGAGCCA
GGTGCCTATATCTTTCTCCAGAACCCCCCAGGTCTGCCTAGCATTGCTGTCTGCTGGTTC
GTGGGCTGCCTTTGTGGAAGCAAGCTCGTCATTGACTGGCACAACTATGGCTACTCCATC
ATGGGTCTGGTGCATGGCCCCAACCATCCCCTCGTTCTGCTGGCCAAGTGGTACGAGAAG
TTCTTTGGGCGCCTGTCCCACCTGAACCTGTGTGTTACCAATGCTATGCGAGAAGACCTG
GCGGATAACTGGCACATCAGGGCTGTGACCGTCTACGACAAGCCCGCATCTTTCTTTAAA
GAGACACCTCTGGACCTGCAGCACCGGCTCTTCATGAAGCTGGGCAGCATGCACTCTCCG
TTCAGGGCCCGCTCAGAACCTGAGGACCCAGTCACGGAGCGGTCGGCCTTCACGGAGCGG
GATGCTGGGAACGGGCTGGTGACGCGTCTCCGTGAGCGGCCAGCCCTGCTGGTCAGCAGC
ACGAGCTGGACAGAGGACGAAGACTTCTCCATCCTGCTGGCAGCTTTAGAAAAGTTTGAA
CAACTGACTCTTGATGGACACAACCTTCCTTCTCTCGTCTGTGTGATAACAGGCAAAGGG
CCTCTGAGGGAGTATTATAGCCGCCTCATCCACCAGAAGCACTTCCAGCACATCCAGGTC
TGCACCCCCTGGCTGGAGGCCGAGGACTACCCCCTGCTTCTAGGGTCGGCGGACCTGGGT
GTCTGTCTGCACACGTCCTCCAGTGGCCTGGACCTGCCCATGAAGGTGGTGGACATGTTC
GGGTGCTGTTTGCCTGTGTGTGCTGTGAACTTCAAGTGTTTACATGAGCTGGTGAAACAT
GAAGAAAATGGCCTGGTCTTTGAGGACTCAGAGGAACTGGCAGCTCAGCTGCAGATGCTT
TTCTCAAACTTTCCTGATCCTGCGGGCAAGCTAAACCAGTTCCGGAAGAACCTGCGGGAG
TCGCAGCAGCTCCGATGGGATGAGAGCTGGGTGCAGACTGTGCTCCCTTTGGTTATGGAC
ACA

Protein sequence predicted based on nucleotide sequence

KRWRRGRAARHVVAVVLGDVGRSPRMQYHALSLAMHGFSVTLLGFCNSKPHDELLQNNRI
QIVGLTELQSLAVGPRVFQYGVKVVLQAMYLLWKLMWREPGAYIFLQNPPGLPSIAVCWF
VGCLCGSKLVIDWHNYGYSIMGLVHGPNHPLVLLAKWYEKFFGRLSHLNLCVTNAMREDL
ADNWHIRAVTVYDKPASFFKETPLDLQHRLFMKLGSMHSPFRARSEPEDPVTERSAFTER
DAGNGLVTRLRERPALLVSSTSWTEDEDFSILLAALEKFEQLTLDGHNLPSLVCVITGKG
PLREYYSRLIHQKHFQHIQVCTPWLEAEDYPLLLGSADLGVCLHTSSSGLDLPMKVVDMF
GCCLPVCAVNFKCLHELVKHEENGLVFEDSEELAAQLQMLFSNFPDPAGKLNQFRKNLRE
SQQLRWDESWVQTVLPLVMDT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

del@1,69

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pGEn2-DEST

Synonyms:

pXLG-NtermTCMhisAviGfp-DEST

Sequencing Primer:

Forward: pGEn2-DEST F

Reverse: pGEn1-DEST R

Description:

Mammalian expression vector with a CMV promoter and N-terminal 8xHis, AviTag, and Super GFP tags; ampicillin resistance in bacteria; Gateway recombinational cloning

Comments:

None

Size (bp):

6930

Parent Vector:

None

Properties:

Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning, with tag/fusion/marker

Author Name:

University of Georgia
Kelley Moremen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCATTAGGCACCCCAGGCTTTACCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAGAGCGCCCAATACGCAAGGAAACAGCTATGACCATGTTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGGAAGGCCCATGAGGCCAGTTAATTAAGAGGTACCTTAATTAAGGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGGAAGATCGCAATTCGCCCTTAAGCTAGCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCTGCAGAAGTTGGTCGTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCAGCGGCCGCCATGAGACTGCTGACCGCCCTGTTCGCCTACTTCATCGTGGCCCTGATCCTGGCCTTCAGCGTGTCCGCCAAGAGCATGCACCACCACCATCACCATCATCACATGAGCGGCCTGAACGACATCTTCGAGGCCCAGAAAATCGAGTGGCACGAGATGAGCAAGGGCGAGGAACTGTTCACCGGCGTGGTGCCCATCCTGGTGGAACTGGACGGCGACGTGAACGGCCACAAGTTCTCTGTGCGGGGCGAGGGCGAGGGGGACGCCACAAATGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGAAAGCTGCCCGTGCCCTGGCCTACCCTGGTCACAACCCTGACCTACGGCGTGCAGTGCTTCAGCAGATACCCCGACCACATGAAGCGGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAAGAACGGACCATCAGCTTCAAGGACGACGGCACCTACAAGACCAGAGCCGAAGTGAAGTTCGAGGGCGACACCCTGGTCAACCGGATCGAGCTGAAGGGCATCGACTTCAAAGAGGACGGCAACATCCTGGGCCACAAGCTCGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGGCACAACGTGGAAGATGGCAGCGTGCAGCTGGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAATCACTACCTGAGCACCCAGAGCGTGCTGAGCAAGGACCCCAACGAGAAGCGGGACCACATGGTGCTGCTGGAATTTGTGACCGCCGCTGGCATCACCCACGGCGAATTCGCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCAGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATAGCTTGGATCCAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGAGATCTGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACTCGAGTTAAGGGCGAATTGCCGATTAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCCTTAATTAAGAGCTCATGGCGCGCCTAGGCCTTGACGGCCTTCCGCCAATTCGCCCTATAGTGAGTCGTATTACGTCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAAACGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGAGCGCCCTGTAGCGGCCACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

Col E1 origin

1137

1804

death cassette

ccdB

ccdB death cassette (removed in plasmids with insert)

5072

5377

intron

artificial intron

2828

2960

poly-A signal

BGH pA

bovine growth hormone (BGH) polyadenylation signal

6106

6320

primer

forward

forward sequencing primer 5? AGCATGCACCACCACCATCACC 3?

3049

3070

primer

reverse

reverse sequencing primer 5' TGACAACGGGCCACAACTCCTC 3'

5729

5750

promoter

CMV

CMV promoter

2208

2790

recombination site

5'ITR

inverted terminal repeat sequence

1998

2127

recombination site

attR1

attR1 recombinational cloning site

3834

3962

recombination site

attR2

attR2 recombinational cloning site

5418

5542

recombination site

3'-ITR

inverted terminal repeat sequence

6408

6537

selectable marker

AmpR

ampicillin resistance

129

1108

selectable marker

CmR

Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)

4071

4720

signal sequence

TCM

TCM signal sequence

2977

3052

tag

His

N-terminal 8xHis tag

3055

3078

tag

AviTag

3079

3129

tag

GFP

N-terminal SuperGFP tag

3130

3825

trxn regulatory element

WPRE

woodchuck hepatitis post-transcriptional regulatory element

5558

6099

Gene

Gene Symbol :

ALG1

Symbol Nomenclature :

ALG1

SYNONYM :

CDG1K; HMAT1; HMT-1; HMT1; MT-1; Mat-1; hMat-1

Designation :

GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase|GDP-mannose-dolichol diphosphochitobiose mannosyltransferase|asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase)|asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog|asparagine-linked glycosylation protein 1 homolog|beta-1,4 mannosyltransferase|beta-1,4-mannosyltransferase|chitobiosyldiphosphodolichol beta-mannosyltransferase|mannosyltransferase-1

Full Nomenclature :

ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase

GENEID :

56052

Locus Tag :

PSEC0061

GenBank Accession :

BC004402

HGNC :

18294

MIM :

605907

Target GenBank:

BC004402

Reference Sequence Alignment

  

HsCD00413048      ------------------------------------------------------------
NM_019109.4       ATGGCGGCCTCATGCTTGGTCCTGCTGGCGCTGTGTCTGCTGCTGCCGCTGCTGCTGCTG
                                                                              

HsCD00413048      ---------AAGCGCTGGCGCCGGGGGCGGGCGGCCCGGCATGTAGTAGCGGTGGTGCTG
NM_019109.4       GGAGGATGGAAGCGCTGGCGCCGGGGGCGGGCGGCCCGGCATGTAGTAGCGGTGGTGCTG
                           ***************************************************

HsCD00413048      GGCGACGTGGGCCGCAGCCCCCGTATGCAGTACCACGCGCTGTCGTTGGCCATGCACGGC
NM_019109.4       GGCGACGTGGGCCGCAGCCCCCGTATGCAGTACCACGCGCTGTCGTTGGCCATGCACGGC
                  ************************************************************

HsCD00413048      TTCTCGGTGACCCTCCTGGGGTTCTGCAACTCCAAACCCCATGATGAGCTCTTGCAGAAC
NM_019109.4       TTCTCGGTGACCCTCCTGGGGTTCTGCAACTCCAAACCCCATGATGAGCTCTTGCAGAAC
                  ************************************************************

HsCD00413048      AACAGAATTCAGATTGTGGGGTTGACAGAACTTCAGAGTCTTGCAGTTGGGCCCCGAGTT
NM_019109.4       AACAGAATTCAGATTGTGGGGTTGACAGAACTTCAGAGTCTTGCAGTTGGGCCCCGAGTT
                  ************************************************************

HsCD00413048      TTCCAGTACGGAGTCAAAGTTGTACTTCAGGCTATGTACTTGCTGTGGAAGTTGATGTGG
NM_019109.4       TTCCAGTACGGAGTCAAAGTTGTACTTCAGGCTATGTACTTGCTGTGGAAGTTGATGTGG
                  ************************************************************

HsCD00413048      AGGGAGCCAGGTGCCTATATCTTTCTCCAGAACCCCCCAGGTCTGCCTAGCATTGCTGTC
NM_019109.4       AGGGAGCCAGGTGCCTATATCTTTCTCCAGAACCCCCCAGGTCTGCCTAGCATTGCTGTC
                  ************************************************************

HsCD00413048      TGCTGGTTCGTGGGCTGCCTTTGTGGAAGCAAGCTCGTCATTGACTGGCACAACTATGGC
NM_019109.4       TGCTGGTTCGTGGGCTGCCTTTGTGGAAGCAAGCTCGTCATTGACTGGCACAACTATGGC
                  ************************************************************

HsCD00413048      TACTCCATCATGGGTCTGGTGCATGGCCCCAACCATCCCCTCGTTCTGCTGGCCAAGTGG
NM_019109.4       TACTCCATCATGGGTCTGGTGCATGGCCCCAACCATCCCCTCGTTCTGCTGGCCAAGTGG
                  ************************************************************

HsCD00413048      TACGAGAAGTTCTTTGGGCGCCTGTCCCACCTGAACCTGTGTGTTACCAATGCTATGCGA
NM_019109.4       TACGAGAAGTTCTTTGGGCGCCTGTCCCACCTGAACCTGTGTGTTACCAATGCTATGCGA
                  ************************************************************

HsCD00413048      GAAGACCTGGCGGATAACTGGCACATCAGGGCTGTGACCGTCTACGACAAGCCCGCATCT
NM_019109.4       GAAGACCTGGCGGATAACTGGCACATCAGGGCTGTGACCGTCTACGACAAGCCCGCATCT
                  ************************************************************

HsCD00413048      TTCTTTAAAGAGACACCTCTGGACCTGCAGCACCGGCTCTTCATGAAGCTGGGCAGCATG
NM_019109.4       TTCTTTAAAGAGACACCTCTGGACCTGCAGCACCGGCTCTTCATGAAGCTGGGCAGCATG
                  ************************************************************

HsCD00413048      CACTCTCCGTTCAGGGCCCGCTCAGAACCTGAGGACCCAGTCACGGAGCGGTCGGCCTTC
NM_019109.4       CACTCTCCGTTCAGGGCCCGCTCAGAACCTGAGGACCCAGTCACGGAGCGGTCGGCCTTC
                  ************************************************************

HsCD00413048      ACGGAGCGGGATGCTGGGAACGGGCTGGTGACGCGTCTCCGTGAGCGGCCAGCCCTGCTG
NM_019109.4       ACGGAGCGGGATGCTGGGAGCGGGCTGGTGACGCGTCTCCGTGAGCGGCCAGCCCTGCTG
                  *******************.****************************************

HsCD00413048      GTCAGCAGCACGAGCTGGACAGAGGACGAAGACTTCTCCATCCTGCTGGCAGCTTTAGAA
NM_019109.4       GTCAGCAGCACGAGCTGGACAGAGGACGAAGACTTCTCCATCCTGCTGGCAGCTTTAGAA
                  ************************************************************

HsCD00413048      AAGTTTGAACAACTGACTCTTGATGGACACAACCTTCCTTCTCTCGTCTGTGTGATAACA
NM_019109.4       AAGTTTGAACAACTGACTCTTGATGGACACAACCTTCCTTCTCTCGTCTGTGTGATAACA
                  ************************************************************

HsCD00413048      GGCAAAGGGCCTCTGAGGGAGTATTATAGCCGCCTCATCCACCAGAAGCACTTCCAGCAC
NM_019109.4       GGCAAAGGGCCTCTGAGGGAGTATTATAGCCGCCTCATCCACCAGAAGCACTTCCAGCAC
                  ************************************************************

HsCD00413048      ATCCAGGTCTGCACCCCCTGGCTGGAGGCCGAGGACTACCCCCTGCTTCTAGGGTCGGCG
NM_019109.4       ATCCAGGTCTGCACCCCCTGGCTGGAGGCCGAGGACTACCCCCTGCTTCTAGGGTCGGCG
                  ************************************************************

HsCD00413048      GACCTGGGTGTCTGTCTGCACACGTCCTCCAGTGGCCTGGACCTGCCCATGAAGGTGGTG
NM_019109.4       GACCTGGGTGTCTGTCTGCACACGTCCTCCAGTGGCCTGGACCTGCCCATGAAGGTGGTG
                  ************************************************************

HsCD00413048      GACATGTTCGGGTGCTGTTTGCCTGTGTGTGCTGTGAACTTCAAGTGTTTACATGAGCTG
NM_019109.4       GACATGTTCGGGTGCTGTTTGCCTGTGTGTGCTGTGAACTTCAAGTGTTTACATGAGCTG
                  ************************************************************

HsCD00413048      GTGAAACATGAAGAAAATGGCCTGGTCTTTGAGGACTCAGAGGAACTGGCAGCTCAGCTG
NM_019109.4       GTGAAACATGAAGAAAATGGCCTGGTCTTTGAGGACTCAGAGGAACTGGCAGCTCAGCTG
                  ************************************************************

HsCD00413048      CAGATGCTTTTCTCAAACTTTCCTGATCCTGCGGGCAAGCTAAACCAGTTCCGGAAGAAC
NM_019109.4       CAGATGCTTTTCTCAAACTTTCCTGATCCTGCGGGCAAGCTAAACCAGTTCCGGAAGAAC
                  ************************************************************

HsCD00413048      CTGCGGGAGTCGCAGCAGCTCCGATGGGATGAGAGCTGGGTGCAGACTGTGCTCCCTTTG
NM_019109.4       CTGCGGGAGTCGCAGCAGCTCCGATGGGATGAGAGCTGGGTGCAGACTGTGCTCCCTTTG
                  ************************************************************

HsCD00413048      GTTATGGACACATAG
NM_019109.4       GTTATGGACACATAA
                  **************.

Pathways

Reactome :

Asparagine N-linked glycosylation

Reactome :

Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein

Reactome :

Metabolism of proteins

Reactome :

Post-translational protein modification

Protein

UniProt :

Q9BT22

HPRD :

10437

Clone

Cloning Information :

581232

Original Clone ID :

HGT33_M01

Organism

Species Specific ID:

56052

Genome

Chromosome :

Map Location :

16p13.3

Reagents

Labome :

ALG1-antibody