Gene:
Gene Symbol:
LCTL
Gene Name:
lactase-like
Original Clone ID:
HGH01_M04
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEn2-DEST
Format:
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00413051
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
CCAGAAGAGGCCTCCTTCTACTATGGAACCTTCCCTCTTGGCTTCTCCTGGGGCGTGGGC
AGTTCTGCCTACCAGACGGAGGGCGCCTGGGACCAGGACGGGAAAGGGCCTAGCATCTGG
GACGTCTTCACACACAGTGGGAAGGGGAAAGTGCTTGGGAATGAGACGGCAGATGTAGCC
TGTGACGGCTACTACAAGGTCCAGGAGGACATCATTCTGCTGAGGGAACTGCACGTCAAC
CACTACCGATTCTCCCTGTCTTGGCCCCGGCTCCTGCCCACAGGCATCCGAGCCGAGCAG
GTGAACAAGAAGGGAATCGAATTCTACAGTGATCTTATCGATGCCCTTCTGAGCAGCAAC
ATCACTCCCATCGTGACCTTGCACCACTGGGATCTGCCACAGCTGCTCCAGGTCAAATAC
GGTGGGTGGCAGAATGTGAGCATGGCCAACTACTTCAGAGACTACGCCAACCTGTGCTTT
GAGGCCTTTGGGGACCGTGTGAAGCACTGGATCACGTTCAGTGATCCTCGGGCAATGGCA
GAAAAAGGCTATGAGACGGGCCACCATGCGCCGGGCCTGAAGCTCCGCGGCACCGGCCTG
TACAAGGCAGCACACCACATCATTAAGGCCCACGCCAAAGCCTGGCATTCTTATAACACC
ACGTGGCGCAGCAAGCAGCAAGGTCTGGTGGGAATTTCATTGAACTGTGACTGGGGGGAA
CCTGTGGACATTAGTAACCCCAAGGACCTAGAGGCTGCCGAGAGATACCTACAGTTCTGT
CTGGGCTGGTTTGCCAACCCCATTTATGCCGGTGACTACCCCCAAGTCATGAAGGACTAC
ATTGGAAGAAAGAGTGCAGAGCAAGGCCTGGAGATGTCGAGGTTACCGGTGTTCTCACTC
CAGGAGAAGAGCTACATTAAAGGCACATCCGATTTCTTGGGATTAGGTCATTTTACTACT
CGGTACATCACGGAAAGGAACTACCCCTCCCGCCAGGGGCCCAGCTACCAGAACGATCGT
GACTTGATAGAGCTGGTTGACCCAAACTGGCCAGATCTGGGGTCTAAATGGCTATATTCT
GTGCCATGGGGATTTAGGAGGCTCCTTAACTTTGCTCAGACTCAATACGGTGATCCTCCC
ATATATGTGATGGAAAATGGAGCATCTCAAAAATTCCACTGTACTCAATTATGTGATGAG
TGGAGAATTCAATACCTTAAAGGATACATAAATGAAATGCTAAAAGCTATAAAAGATGGT
GCTAATATAAAGGGGTATACTTCCTGGTCTCTGTTGGATAAGTTTGAATGGGAGAAAGGA
TACTCAGATAGATATGGATTCTACTATGTTGAATTTAACGACAGAAATAAGCCTCGCTAT
CCAAAGGCTTCAGTTCAATATTACAAGAAGATTATCATTGCCAATGGGTTTCCCAATCCA
AGAGAGGTGGAAAGTTGGTACCTCAAAGCTTTGGAAACTTGCTCTATCAACAATCAGATG
CTTGCTGCAGAGCCCTTGCTAAGTCACATGCAAATGGTTACGGAG
Protein sequence predicted based on nucleotide sequence
PEEASFYYGTFPLGFSWGVGSSAYQTEGAWDQDGKGPSIWDVFTHSGKGKVLGNETADVA CDGYYKVQEDIILLRELHVNHYRFSLSWPRLLPTGIRAEQVNKKGIEFYSDLIDALLSSN ITPIVTLHHWDLPQLLQVKYGGWQNVSMANYFRDYANLCFEAFGDRVKHWITFSDPRAMA EKGYETGHHAPGLKLRGTGLYKAAHHIIKAHAKAWHSYNTTWRSKQQGLVGISLNCDWGE PVDISNPKDLEAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSL QEKSYIKGTSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYS VPWGFRRLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDG ANIKGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNP REVESWYLKALETCSINNQMLAAEPLLSHMQMVTE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,78; del@1627,75
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGEn2-DEST
Synonyms:
pXLG-NtermTCMhisAviGfp-DEST
Sequencing Primer:
Forward:
pGEn2-DEST F
Reverse:
pGEn1-DEST R
Description:
Mammalian expression vector with a CMV promoter and N-terminal 8xHis, AviTag, and Super GFP tags; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments:
None
Size (bp):
6930
Parent Vector:
None
Properties:
Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
Col E1 origin
1137
1804
death cassette
ccdB
ccdB death cassette (removed in plasmids with insert)
5072
5377
intron
artificial intron
artificial intron
2828
2960
poly-A signal
BGH pA
bovine growth hormone (BGH) polyadenylation signal
6106
6320
primer
forward
forward sequencing primer 5? AGCATGCACCACCACCATCACC 3?
3049
3070
primer
reverse
reverse sequencing primer 5' TGACAACGGGCCACAACTCCTC 3'
5729
5750
promoter
CMV
CMV promoter
2208
2790
recombination site
5'ITR
inverted terminal repeat sequence
1998
2127
recombination site
attR1
attR1 recombinational cloning site
3834
3962
recombination site
attR2
attR2 recombinational cloning site
5418
5542
recombination site
3'-ITR
inverted terminal repeat sequence
6408
6537
selectable marker
AmpR
ampicillin resistance
129
1108
selectable marker
CmR
Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)
4071
4720
signal sequence
TCM
TCM signal sequence
2977
3052
tag
His
N-terminal 8xHis tag
3055
3078
tag
AviTag
AviTag
3079
3129
tag
GFP
N-terminal SuperGFP tag
3130
3825
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
5558
6099
