CTBS (Homo sapiens) in pGEn2-DEST

Explanation of Terms

Gene:
Gene Symbol:  CTBS
Gene Name:  chitobiase, di-N-acetyl-
Original Clone ID:
HGH18_M07
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEn2-DEST
Format: 
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00413066
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1041nts
Open reading frame: 1 to 1041

ACCGACTGCCCATGCCCGGAGCCTGAGCTCTGCCGCCCGATTCGCCACCATCCAGATTTC
GAGGTCTTTGTGTTTGATGTTGGACAGAAAACTTGGAAATCTTATGATTGGTCACAGATT
ACAACTGTGGCAACATTTGGAAAATATGACTCAGAACTTATGTGCTACGCTCATTCAAAA
GGAGCCAGAGTAGTACTTAAAGGAGATGTATCCTTAAAGGATATCATTGATCCTGCTTTC
AGAGCATCCTGGATAGCTCAAAAACTTAATTTGGCCAAAACACAATATATGGATGGAATT
AATATAGATATAGAGCAAGAAGTTAATTGTTTATCACCTGAATATGATGCATTAACTGCT
TTAGTCAAAGAAACTACAGACTCTTTCCATCGTGAAATTGAGGGATCACAGGTAACCTTT
GATGTAGCTTGGTCTCCAAAGAACATAGACAGAAGATGCTATAATTATACTGGAATCGCA
GATGCTTGTGACTTCCTCTTTGTGATGTCTTATGATGAACAAAGTCAGATCTGGTCAGAA
TGTATTGCAGCAGCCAATGCTCCCTATAATCAGACATTAACTGGATATAATGACTACATC
AAGATGAGCATTAATCCTAAGAAACTTGTAATGGGTGTTCCTTGGTATGGTTATGATTAT
ACCTGCCTGAATCTGTCTGAGGATCATGTTTGTACCATTGCAAAAGTCCCTTTCCGGGGG
GCTCCTTGTAGTGACGCTGCAGGACGTCAGGTGCCCTACAAAACGATCATGAAGCAAATA
AATAGTTCTATTTCTGGAAACCTATGGGATAAAGATCAGCGGGCTCCTTATTATAACTAT
AAAGATCCTGCTGGCCACTTTCATCAAGTATGGTATGATAACCCTCAGAGTATTTCTTTA
AAGGCAACATATATACAAAACTATCGCTTACGGGGCATTGGCATGTGGAATGCAAACTGT
CTTGACTACTCTGGAGATGCTGTAGCCAAACAGCAAACTGAAGAAATGTGGGAAGTCTTA
AAGCCAAAGCTGTTACAGAGA

Protein sequence predicted based on nucleotide sequence

TDCPCPEPELCRPIRHHPDFEVFVFDVGQKTWKSYDWSQITTVATFGKYDSELMCYAHSK
GARVVLKGDVSLKDIIDPAFRASWIAQKLNLAKTQYMDGINIDIEQEVNCLSPEYDALTA
LVKETTDSFHREIEGSQVTFDVAWSPKNIDRRCYNYTGIADACDFLFVMSYDEQSQIWSE
CIAAANAPYNQTLTGYNDYIKMSINPKKLVMGVPWYGYDYTCLNLSEDHVCTIAKVPFRG
APCSDAAGRQVPYKTIMKQINSSISGNLWDKDQRAPYYNYKDPAGHFHQVWYDNPQSISL
KATYIQNYRLRGIGMWNANCLDYSGDAVAKQQTEEMWEVLKPKLLQR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,114

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable