SPAM1 (Homo sapiens) in pGEn2-DEST

Explanation of Terms

Gene:
Gene Symbol:  SPAM1
Gene Name:  sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
Original Clone ID:
HGH56_M03
Keyword:
partial cds (N and C terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEn2-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00413098
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1365nts
Open reading frame: 1 to 1365

CTGAATTTCAGAGCACCTCCTGTTATTCCAAATGTGCCTTTCCTCTGGGCCTGGAATGCC
CCAAGTGAATTTTGTCTTGGAAAATTTGATGAGCCACTAGATATGAGCCTCTTCTCTTTC
ATAGGAAGCCCCCGAATAAACGCCACCGGGCAAGGTGTTACAATATTTTATGTTGATAGA
CTTGGCTACTATCCTTACATAGATTCAATCACAGGAGTAACTGTGAATGGAGGAATCCCC
CAGAAGATTTCCTTACAAGACCATCTGGACAAAGCTAAGAAAGACATTACATTTTATATG
CCAGTAGACAATTTGGGAATGGCTGTTATTGACTGGGAAGAATGGAGACCCACTTGGGCA
AGAAACTGGAAACCTAAAGATGTTTACAAGAATAGGTCTATTGAATTGGTTCAGCAACAA
AATGTACAACTTAGTCTCACAGAGGCCACTGAGAAAGCAAAACAAGAATTTGAAAAAGCA
GGGAAGGATTTCCTGGTAGAGACTATAAAATTGGGAAAATTACTTCGGCCAAATCACTTG
TGGGGTTATTATCTTTTTCCGGATTGTTACAACCATCACTATAAGAAACCCGGTTACAAT
GGAAGTTGCTTCAATGTAGAAATAAAAAGAAATGATGATCTCAGCTGGTTGTGGAATGAA
AGCACTGCTCTTTACCCATCCATTTATTTGAACACTCAGCAGTCTCCTGTAGCTGCTACA
CTCTATGTGCGCAATCGAGTTCGGGAAGCCATCAGAGTTTCCAAAATACCTGATGCAAAA
AGTCCACTTCCGGTTTTTGCATATACCCGCATAGTTTTTACTGATCAAGTTTTGAAATTC
CTTTCTCAAGATGAACTTGTGTATACATTTGGCGAAACTGTTGCTCTGGGTGCTTCTGGA
ATTGTAATATGGGGAACCCTCAGTATAATGCGAAGTATGAAATCTTGCTTGCTCCTAGAC
AATTACATGGAGACTATACTGAATCCTTACATAATCAACGTCACACTAGCAGCCAAAATG
TGTAGCCAAGTGCTTTGCCAGGAGCAAGGAGTGTGTATAAGGAAAAACTGGAATTCAAGT
GACTATCTTCACCTCAACCCAGATAATTTTGCTATTCAACTTGAGAAAGGTGGAAAGTTC
ACAGTACGTGGAAAACCGACACTTGAAGACCTGGAGCAATTTTCTGAAAAATTTTATTGC
AGCTGTTATAGCACCTTGAGTTGTAAGGAGAAAGCTGATGTAAAAGACACTGATGCTGTT
GATGTGTGTATTGCTGATGGTGTCTGTATAGATGCTTTTCTAAAACCTCCCATGGAGACA
GAAGAACCTCAAATTTTCTACAATGCTTCACCCTCCACACTATAG

Protein sequence predicted based on nucleotide sequence

LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDR
LGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWA
RNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHL
WGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAAT
LYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASG
IVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSS
DYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAV
DVCIADGVCIDAFLKPPMETEEPQIFYNASPSTL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,105; del@1471,66

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable