DNASU Plasmid | HYAL4 (Homo sapiens) in pGEn2-DEST

Explanation of Terms

Gene:

Gene Symbol: HYAL4

Gene Name: hyaluronoglucosaminidase 4

Original Clone ID:

HGH56_M07

Keyword:

partial cds (N and C terminal deletion)

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pGEn2-DEST

Format:

CLOSED

Source:

Glyco-enzyme clone collection

Description:

All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen

HsCD00413119

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 1275nts

Open reading frame: 1 to 1275

Reference Sequence Alignment

AAGTCTATCTCTTGTCTAAAACCTGCTCGACTTCCAATTTATCAAAGGAAACCTTTTATA
GCTGCTTGGAATGCTCCAACAGATCAGTGTTTGATAAAATATAATTTAAGACTAAATTTG
AAAATGTTTCCTGTGATTGGAAGCCCACTGGCCAAGGCCAGGGGGCAAAATGTCACTATA
TTTTATGTCAACAGATTGGGATACTATCCGTGGTATACATCACAAGGGGTCCCCATTAAT
GGAGGTCTCCCACAGAACATAAGTTTACAAGTACATCTGGAAAAAGCTGACCAAGATATT
AATTATTACATCCCTGCTGAAGATTTCAGTGGACTTGCTGTTATAGATTGGGAATATTGG
CGACCACAGTGGGCCCGGAACTGGAACTCAAAAGATGTTTACAGACAGAAGTCAAGAAAG
CTTATTTCCGATATGGGAAAGAATGTATCAGCTACCGATATTGAATATTTAGCCAAAGTG
ACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAGGAAACCATCAAATTGGGAATTAAGAGC
CGACCCAAAGGCCTTTGGGGTTATTATTTATATCCTGATTGCCACAATTATAACGTTTAT
GCCCCAAACTACTCTGGGTCATGCCCAGAAGACGAAGTCTTGAGGAACAATGAGCTCTCT
TGGCTCTGGAACAGCAGTGCTGCTTTATATCCTTCTATCGGTGTCTGGAAATCCCTTGGA
GACAGTGAAAACATTTTGCGCTTCTCCAAATTTCGGGTGCATGAATCCATGAGGATCTCC
ACCATGACATCTCATGATTATGCTCTGCCTGTATTTGTCTACACAAGGCTAGGGTACAGA
GATGAACCTTTATTTTTCCTTTCTAAGCAAGATCTAGTCAGCACCATAGGAGAAAGTGCT
GCCTTGGGAGCTGCAGGCATTGTTATTTGGGGAGACATGAATTTAACTTCATCCAAGGCC
AACTGTACAAAGGTGAAGCAGTTTGTGAGTTCTGATTTAGGGAGCTACATAGCCAATGTG
ACCAGAGCTGCTGAGGTATGCAGCCTTCACCTCTGCAGGAACAATGGCAGGTGCATAAGG
AAGATGTGGAACGCGCCCAGTTACCTTCACTTGAACCCTGCAAGTTACCACATAGAGGCC
TCTGAGGACGGGGAGTTTACTGTGAAAGGAAAAGCATCTGATACAGACCTGGCAGTGATG
GCAGATACATTTTCCTGTCATTGTTATCAGGGATATGAAGGAGCTGATTGCAGAGAAATA
AAGACGGCTGATTAG

Protein sequence predicted based on nucleotide sequence

KSISCLKPARLPIYQRKPFIAAWNAPTDQCLIKYNLRLNLKMFPVIGSPLAKARGQNVTI
FYVNRLGYYPWYTSQGVPINGGLPQNISLQVHLEKADQDINYYIPAEDFSGLAVIDWEYW
RPQWARNWNSKDVYRQKSRKLISDMGKNVSATDIEYLAKVTFEESAKAFMKETIKLGIKS
RPKGLWGYYLYPDCHNYNVYAPNYSGSCPEDEVLRNNELSWLWNSSAALYPSIGVWKSLG
DSENILRFSKFRVHESMRISTMTSHDYALPVFVYTRLGYRDEPLFFLSKQDLVSTIGESA
ALGAAGIVIWGDMNLTSSKANCTKVKQFVSSDLGSYIANVTRAAEVCSLHLCRNNGRCIR
KMWNAPSYLHLNPASYHIEASEDGEFTVKGKASDTDLAVMADTFSCHCYQGYEGADCREI
KTAD*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

del@1,87; del@1363,84

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pGEn2-DEST

Synonyms:

pXLG-NtermTCMhisAviGfp-DEST

Sequencing Primer:

Forward: pGEn2-DEST F

Reverse: pGEn1-DEST R

Description:

Mammalian expression vector with a CMV promoter and N-terminal 8xHis, AviTag, and Super GFP tags; ampicillin resistance in bacteria; Gateway recombinational cloning

Comments:

None

Size (bp):

6930

Parent Vector:

None

Properties:

Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning, with tag/fusion/marker

Author Name:

University of Georgia
Kelley Moremen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCATTAGGCACCCCAGGCTTTACCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAGAGCGCCCAATACGCAAGGAAACAGCTATGACCATGTTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGGAAGGCCCATGAGGCCAGTTAATTAAGAGGTACCTTAATTAAGGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGGAAGATCGCAATTCGCCCTTAAGCTAGCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCTGCAGAAGTTGGTCGTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCAGCGGCCGCCATGAGACTGCTGACCGCCCTGTTCGCCTACTTCATCGTGGCCCTGATCCTGGCCTTCAGCGTGTCCGCCAAGAGCATGCACCACCACCATCACCATCATCACATGAGCGGCCTGAACGACATCTTCGAGGCCCAGAAAATCGAGTGGCACGAGATGAGCAAGGGCGAGGAACTGTTCACCGGCGTGGTGCCCATCCTGGTGGAACTGGACGGCGACGTGAACGGCCACAAGTTCTCTGTGCGGGGCGAGGGCGAGGGGGACGCCACAAATGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGAAAGCTGCCCGTGCCCTGGCCTACCCTGGTCACAACCCTGACCTACGGCGTGCAGTGCTTCAGCAGATACCCCGACCACATGAAGCGGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAAGAACGGACCATCAGCTTCAAGGACGACGGCACCTACAAGACCAGAGCCGAAGTGAAGTTCGAGGGCGACACCCTGGTCAACCGGATCGAGCTGAAGGGCATCGACTTCAAAGAGGACGGCAACATCCTGGGCCACAAGCTCGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGGCACAACGTGGAAGATGGCAGCGTGCAGCTGGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAATCACTACCTGAGCACCCAGAGCGTGCTGAGCAAGGACCCCAACGAGAAGCGGGACCACATGGTGCTGCTGGAATTTGTGACCGCCGCTGGCATCACCCACGGCGAATTCGCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCAGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATAGCTTGGATCCAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGAGATCTGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACTCGAGTTAAGGGCGAATTGCCGATTAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCCTTAATTAAGAGCTCATGGCGCGCCTAGGCCTTGACGGCCTTCCGCCAATTCGCCCTATAGTGAGTCGTATTACGTCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAAACGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGAGCGCCCTGTAGCGGCCACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

Col E1 origin

1137

1804

death cassette

ccdB

ccdB death cassette (removed in plasmids with insert)

5072

5377

intron

artificial intron

2828

2960

poly-A signal

BGH pA

bovine growth hormone (BGH) polyadenylation signal

6106

6320

primer

forward

forward sequencing primer 5? AGCATGCACCACCACCATCACC 3?

3049

3070

primer

reverse

reverse sequencing primer 5' TGACAACGGGCCACAACTCCTC 3'

5729

5750

promoter

CMV

CMV promoter

2208

2790

recombination site

5'ITR

inverted terminal repeat sequence

1998

2127

recombination site

attR1

attR1 recombinational cloning site

3834

3962

recombination site

attR2

attR2 recombinational cloning site

5418

5542

recombination site

3'-ITR

inverted terminal repeat sequence

6408

6537

selectable marker

AmpR

ampicillin resistance

129

1108

selectable marker

CmR

Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)

4071

4720

signal sequence

TCM

TCM signal sequence

2977

3052

tag

His

N-terminal 8xHis tag

3055

3078

tag

AviTag

3079

3129

tag

GFP

N-terminal SuperGFP tag

3130

3825

trxn regulatory element

WPRE

woodchuck hepatitis post-transcriptional regulatory element

5558

6099

Gene

Gene Symbol :

HYAL4

Symbol Nomenclature :

HYAL4

SYNONYM :

CSHY

Designation :

Full Nomenclature :

hyaluronoglucosaminidase 4

GENEID :

23553

GenBank Accession :

BC104788

HGNC :

5323

MIM :

604510

Vega :

OTTHUMG00000157349

Target GenBank:

BC104788

Reference Sequence Alignment

  

HsCD00413119        ------------------------------------------------------------
XM_005250238.1      ATGAAAGTATTATCTGAAGGACAGTTAAAGCTTTGTGTTGTTCAACCAGTACATCTCACT
NM_012269.2         ATGAAAGTATTATCTGAAGGACAGTTAAAGCTTTGTGTTGTTCAACCAGTACATCTCACT
                                                                                

HsCD00413119        ---------------------------AAGTCTATCTCTTGTCTAAAACCTGCTCGACTT
XM_005250238.1      TCATGGCTCCTTATATTTTTTATTCTAAAGTCTATCTCTTGTCTAAAACCTGCTCGACTT
NM_012269.2         TCATGGCTCCTTATATTTTTTATTCTAAAGTCTATCTCTTGTCTAAAACCTGCTCGACTT
                                               *********************************

HsCD00413119        CCAATTTATCAAAGGAAACCTTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTG
XM_005250238.1      CCAATTTATCAAAGGAAACCTTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTG
NM_012269.2         CCAATTTATCAAAGGAAACCTTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTG
                    ************************************************************

HsCD00413119        ATAAAATATAATTTAAGACTAAATTTGAAAATGTTTCCTGTGATTGGAAGCCCACTGGCC
XM_005250238.1      ATAAAATATAATTTAAGACTAAATTTGAAAATGTTTCCTGTGATTGGAAGCCCACTGGCC
NM_012269.2         ATAAAATATAATTTAAGACTAAATTTGAAAATGTTTCCTGTGATTGGAAGCCCACTGGCC
                    ************************************************************

HsCD00413119        AAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACAGATTGGGATACTATCCGTGG
XM_005250238.1      AAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACAGATTGGGATACTATCCGTGG
NM_012269.2         AAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACAGATTGGGATACTATCCGTGG
                    ************************************************************

HsCD00413119        TATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAGTTTACAAGTA
XM_005250238.1      TATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAGTTTACAAGTA
NM_012269.2         TATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAGTTTACAAGTA
                    ************************************************************

HsCD00413119        CATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA
XM_005250238.1      CATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA
NM_012269.2         CATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA
                    ************************************************************

HsCD00413119        CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAA
XM_005250238.1      CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAA
NM_012269.2         CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAA
                    ************************************************************

HsCD00413119        GATGTTTACAGACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCT
XM_005250238.1      GATGTTTACAGACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCT
NM_012269.2         GATGTTTACAGACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCT
                    ************************************************************

HsCD00413119        ACCGATATTGAATATTTAGCCAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAG
XM_005250238.1      ACCGATATTGAATATTTAGCCAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAG
NM_012269.2         ACCGATATTGAATATTTAGCCAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAG
                    ************************************************************

HsCD00413119        GAAACCATCAAATTGGGAATTAAGAGCCGACCCAAAGGCCTTTGGGGTTATTATTTATAT
XM_005250238.1      GAAACCATCAAATTGGGAATTAAGAGCCGACCCAAAGGCCTTTGGGGTTATTATTTATAT
NM_012269.2         GAAACCATCAAATTGGGAATTAAGAGCCGACCCAAAGGCCTTTGGGGTTATTATTTATAT
                    ************************************************************

HsCD00413119        CCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACTCTGGGTCATGCCCAGAAGAC
XM_005250238.1      CCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACTCTGGGTCATGCCCAGAAGAC
NM_012269.2         CCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACTCTGGGTCATGCCCAGAAGAC
                    ************************************************************

HsCD00413119        GAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGCTTTATATCCT
XM_005250238.1      GAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGCTTTATATCCT
NM_012269.2         GAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGCTTTATATCCT
                    ************************************************************

HsCD00413119        TCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT
XM_005250238.1      TCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT
NM_012269.2         TCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT
                    ************************************************************

HsCD00413119        CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTA
XM_005250238.1      CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTA
NM_012269.2         CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTA
                    ************************************************************

HsCD00413119        TTTGTCTACACAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGAT
XM_005250238.1      TTTGTCTACACAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGAT
NM_012269.2         TTTGTCTACACAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGAT
                    ************************************************************

HsCD00413119        CTAGTCAGCACCATAGGAGAAAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGA
XM_005250238.1      CTAGTCAGCACCATAGGAGAAAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGA
NM_012269.2         CTAGTCAGCACCATAGGAGAAAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGA
                    ************************************************************

HsCD00413119        GACATGAATTTAACTTCATCCAAGGCCAACTGTACAAAGGTGAAGCAGTTTGTGAGTTCT
XM_005250238.1      GACATGAATTTAACTGCATCCAAGGCCAACTGTACAAAGGTGAAGCAGTTTGTGAGTTCT
NM_012269.2         GACATGAATTTAACTGCATCCAAGGCCAACTGTACAAAGGTGAAGCAGTTTGTGAGTTCT
                    *************** ********************************************

HsCD00413119        GATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTGAGGTATGCAGCCTTCACCTC
XM_005250238.1      GATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTGAGGTATGCAGCCTTCACCTC
NM_012269.2         GATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTGAGGTATGCAGCCTTCACCTC
                    ************************************************************

HsCD00413119        TGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTACCTTCACTTG
XM_005250238.1      TGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTACCTTCACTTG
NM_012269.2         TGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTACCTTCACTTG
                    ************************************************************

HsCD00413119        AACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA
XM_005250238.1      AACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA
NM_012269.2         AACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA
                    ************************************************************

HsCD00413119        GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGA
XM_005250238.1      GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGA
NM_012269.2         GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGA
                    ************************************************************

HsCD00413119        TATGAAGGAGCTGATTGCAGAGAAATAAAGACGGCTGATTAG------------------
XM_005250238.1      TATGAAGGAGCTGATTGCAGAGAAATAAAGACGGCTGATGGCTGCTCTGGGGTTTCCCCT
NM_012269.2         TATGAAGGAGCTGATTGCAGAGAAATAAAGACGGCTGATGGCTGCTCTGGGGTTTCCCCT
                    *************************************** .                   

HsCD00413119        ------------------------------------------------------------
XM_005250238.1      TCTCCTGGTTCACTAATGACACTTTGTCTACTGCTTTTAGCAAGTTATCGAAGCATTCAG
NM_012269.2         TCTCCTGGTTCACTAATGACACTTTGTCTACTGCTTTTAGCAAGTTATCGAAGCATTCAG
                                                                                

HsCD00413119        ------
XM_005250238.1      TTGTGA
NM_012269.2         TTGTGA