MAN1A2 (Homo sapiens) in pGEn2-DEST

Explanation of Terms

Gene:
Gene Symbol:  MAN1A2
Gene Name:  mannosidase, alpha, class 1A, member 2
Original Clone ID:
HGH47_M02
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEn2-DEST
Format: 
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00413127
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1677nts
Open reading frame: 1 to 1677

GCTAAAAACCCCGGAGTCTTCCTGATCCATGGACCCGATGAACATAGACACAGGGAAGAG
GAAGAACGTCTGAGAAATAAAATTCGAGCTGATCATGAGAAGGCCTTGGAAGAAGCAAAA
GAAAAATTAAGAAAGTCAAGAGAGGAAATTCGAGCAGAAATTCAGACAGAGAAAAATAAG
GTAGTCCAAGAAATGAAGATAAAAGAGAACAAGCCACTGCCACCAGTCCCTATTCCCAAC
CTTGTAGGAATACGTGGTGGAGACCCAGAAGATAATGACATAAGAGAGAAAAGGGAAAAA
ATTAAAGAGATGATGAAACATGCTTGGGATAACTATAGGACATATGGGTGGGGACATAAT
GAACTCAGACCTATTGCAAGGAAAGGACACTCCCCTAACATATTTGGAAGTTCACAAATG
GGTGCTACCATAGTAGATGCTTTGGATACCCTTTATATCATGGGACTTCATGATGAATTC
CTAGATGGGCAAAGATGGATTGAAGACAACCTTGATTTCAGTGTGAATTCAGAGGTGTCT
GTGTTTGAAGTCAACATTCGATTTATTGGAGGCCTACTTGCAGCATATTACCTATCAGGA
GAGGAGATATTCAAGATTAAAGCAGTGCAATTGGCTGAGAAACTCCTTCCTGCCTTTAAC
ACACCTACTGGGATTCCTTGGGCAATGGTGAATTTGAAAAGTGGAGTAGGGCGAAACTGG
GGCTGGGCATCTGCAGGTAGCAGCATTCTGGCTGAATTTGGTACACTACATATGGAGTTC
ATCCACCTCAGCTACTTGACAGGGGACCTGACTTACTACAAAAAGGTTATGCACATTCGG
AAACTACTTCAGAAAATGGATCGTCCAAATGGTCTTTATCCAAATTATTTGAACCCCAGA
ACAGGGCGCTGGGGTCAGTATCATACATCTGTCGGTGGCCTGGGAGACAGTTTTTATGAA
TACTTACTGAAAGCATGGTTGATGTCAGATAAAACAGACCATGAGGCAAGAAAGATGTAT
GATGATGCTATTGAGGCTATAGAAAAACATCTTATTAAGAAGTCTCGTGGAGGTCTTACC
TTTATTGGAGAATGGAAGAATGGGCACTTGGAAAAAAAGATGGGGCATTTGGCCTGCTTT
GCTGGGGGAATGTTTGCACTAGGAGCAGATGGTTCCAGAGCAGATAAAGCTGGTCATTAT
TTAGAGCTAGGGGCAGAAATTGCACGTACTTGTCATGAGTCATATGACAGAACTGCATTA
AAGCTAGGTCCTGAATCATTCAAGTTTGATGGTGCAGTGGAGGCTGTGGCTGTCCGGCAG
GCTGAAAAGTATTATATCCTCCGTCCAGAAGTAATTGAAACCTATTGGTACCTATGGCGA
TTCACTCACGATCCAAGATACAGGCAGTGGGGCTGGGAAGCAGCACTGGCCATTGAAAAG
TATTGCCGAGTTAATGGTGGGTTTTCTGGAGTCAAAGATGTATATTCCTCTACTCCTACA
CATGATGATGTACAGCAGAGCTTTTTTCTTGCTGAAACATTAAAATATTTGTATCTGCTG
TTCTCCGGTGATGACCTTTTACCTTTAGACCACTGGGTGTTTAATACAGAGGCTCACCCT
CTGCCTGTGTTACATTTAGCCAACACCACACTTTCAGGTAATCCTGCTGTTCGATAG

Protein sequence predicted based on nucleotide sequence

AKNPGVFLIHGPDEHRHREEEERLRNKIRADHEKALEEAKEKLRKSREEIRAEIQTEKNK
VVQEMKIKENKPLPPVPIPNLVGIRGGDPEDNDIREKREKIKEMMKHAWDNYRTYGWGHN
ELRPIARKGHSPNIFGSSQMGATIVDALDTLYIMGLHDEFLDGQRWIEDNLDFSVNSEVS
VFEVNIRFIGGLLAAYYLSGEEIFKIKAVQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNW
GWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPR
TGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLT
FIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTAL
KLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEK
YCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHP
LPVLHLANTTLSGNPAVR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,249

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable