HPSE2 (Homo sapiens) in pGEn2-DEST

Explanation of Terms

Gene:
Gene Symbol:  HPSE2
Gene Name:  heparanase 2
Original Clone ID:
HGH79_M02
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEn2-DEST
Format: 
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00413154
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1776nts
Open reading frame: 1 to 1776

ATGAGGGTGCTTTGTGCCTTCCCTGAAGCCATGCCCTCCAGCAACTCCCGCCCCCCCGCG
TGCCTAGCCCCGGGGGCTCTCTACTTGGCTCTGTTGCTCCATCTCTCCCTTTCCTCCCAG
GCTGGAGACAGGAGACCCTTGCCTGTAGACAGAGCTGCAGGTTTGAAGGAAAAGACCCTG
ATTCTACTTGATGTGAGCACCAAGAACCCAGTCAGGACAGTCAATGAGAACTTCCTCTCT
CTGCAGCTGGATCCGTCCATCATTCATGATGGCTGGCTCGATTTCCTAAGCTCCAAGCGC
TTGGTGACCCTGGCCCGGGGACTTTCGCCCGCCTTTCTGCGCTTCGGGGGCAAAAGGACC
GACTTCCTGCAGTTCCAGAACCTGAGGAACCCGGCGAAAAGCCGCGGGGGCCCGGGCCCG
GATTACTATCTCAAAAACTATGAGGATGACATTGTTCGAAGTGATGTTGCCTTAGATAAA
CAGAAAGGCTGCAAGATTGCCCAGCACCCTGATGTTATGCTGGAGCTCCAAAGGGAGAAG
GCAGCTCAGATGCATCTGGTTCTTCTAAAGGAGCAATTCTCCAATACTTACAGTAATCTC
ATATTAACAGCCAGGTCTCTAGACAAACTTTATAACTTTGCTGATTGCTCTGGACTCCAC
CTGATATTTGCTCTAAATGCACTGCGTCGTAATCCCAATAACTCCTGGAACAGTTCTAGT
GCCCTGAGTCTGTTGAAGTACAGCGCCAGCAAAAAGTACAACATTTCTTGGGAACTGGGT
AATGAGCCAAATAACTATCGGACCATGCATGGCCGGGCAGTAAATGGCAGCCAGTTGGGA
AAGGATTACATCCAGCTGAAGAGCCTGTTGCAGCCCATCCGGATTTATTCCAGAGCCAGC
TTATATGGCCCTAATATTGGGCGGCCGAGGAAGAATGTCATCGCCCTCCTAGATGGATTC
ATGAAGGTGGCAGGAAGTACAGTAGATGCAGTTACCTGGCAACATTGCTACATTGATGGC
CGGGTGGTCAAGGTGATGGACTTCCTGAAAACTCGCCTGTTAGACACACTCTCTGACCAG
ATTAGGAAAATTCAGAAAGTGGTTAATACATACACTCCAGGAAAGAAGATTTGGCTTGAA
GGTGTGGTGACCACCTCAGCTGGAGGCACAAACAATCTATCCGATTCCTATGCTGCAGGA
TTCTTATGGTTGAACACTTTAGGAATGCTGGCCAATCAGGGCATTGATGTCGTGATACGG
CACTCATTTTTTGACCATGGATACAATCACCTCGTGGACCAGAATTTTAACCCATTACCA
GACTACTGGCTCTCTCTCCTCTACAAGCGCCTGATCGGCCCCAAAGTCTTGGCTGTGCAT
GTGGCTGGGCTCCAGCGGAAGCCACGGCCTGGCCGAGTGATCCGGGACAAACTAAGGATT
TATGCTCACTGCACAAACCACCACAACCACAACTACGTTCGTGGGTCCATTACACTTTTT
ATCATCAACTTGCATCGATCAAGAAAGAAAATCAAGCTGACTGGGACTCTCAGAGACAAG
CTGGTTCACCAGTACCTGCTGCAGCCCTATGGGCAGGAGGGCCTAAAGTCCAAGTCAGTG
CAACTGAATGGCCAGCCCTTAGTGATGGTGGACGACGGGACCCTCCCAGAATTGAAGCCC
CGCCCCCTTCGGGCCGGCCGGACATTGGTCATCCCTCCAGTCACCATGGGCTTTTTTGTG
GTCAAGAATGTCAATGCTTTGGCCTGCCGCTACCGA

Protein sequence predicted based on nucleotide sequence

MRVLCAFPEAMPSSNSRPPACLAPGALYLALLLHLSLSSQAGDRRPLPVDRAAGLKEKTL
ILLDVSTKNPVRTVNENFLSLQLDPSIIHDGWLDFLSSKRLVTLARGLSPAFLRFGGKRT
DFLQFQNLRNPAKSRGGPGPDYYLKNYEDDIVRSDVALDKQKGCKIAQHPDVMLELQREK
AAQMHLVLLKEQFSNTYSNLILTARSLDKLYNFADCSGLHLIFALNALRRNPNNSWNSSS
ALSLLKYSASKKYNISWELGNEPNNYRTMHGRAVNGSQLGKDYIQLKSLLQPIRIYSRAS
LYGPNIGRPRKNVIALLDGFMKVAGSTVDAVTWQHCYIDGRVVKVMDFLKTRLLDTLSDQ
IRKIQKVVNTYTPGKKIWLEGVVTTSAGGTNNLSDSYAAGFLWLNTLGMLANQGIDVVIR
HSFFDHGYNHLVDQNFNPLPDYWLSLLYKRLIGPKVLAVHVAGLQRKPRPGRVIRDKLRI
YAHCTNHHNHNYVRGSITLFIINLHRSRKKIKLTGTLRDKLVHQYLLQPYGQEGLKSKSV
QLNGQPLVMVDDGTLPELKPRPLRAGRTLVIPPVTMGFFVVKNVNALACRYR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable