DNASU Plasmid | FUCA1 (Homo sapiens) in pGEn2-DEST

Explanation of Terms

Gene:

Gene Symbol: FUCA1

Gene Name: fucosidase, alpha-L- 1, tissue

Original Clone ID:

HGH29_M02

Keyword:

partial cds (N-terminal deletion)

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pGEn2-DEST

Format:

CLOSED

Source:

Glyco-enzyme clone collection

Description:

All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen

HsCD00413205

Price:

AVAILABLE

No restriction
Special MTA: SPTA

Insert Sequence
Protein Sequence

Insert sequence: 1308nts

Open reading frame: 1 to 1308

Reference Sequence Alignment

CAGCCTCCGCGCCGCTACACCCCAGACTGGCCGAGCCTGGATTCTCGGCCGCTGCCGGCC
TGGTTCGACGAAGCCAAGTTCGGGGTGTTCATCCACTGGGGCGTGTTCTCGGTGCCCGCC
TGGGGCAGCGAGTGGTTCTGGTGGCACTGGCAGGGCGAGGGGCGGCCGCAGTACCAGCGC
TTCATGCGCGACAACTACCCGCCCGGCTTCAGCTACGCCGACTTCGGACCGCAGTTCACT
GCGCGCTTCTTCCACCCGGAGGAGTGGGCCGACCTCTTCCAGGCCGCGGGCGCCAAGTAT
GTAGTTTTGACGACAAAGCATCACGAAGGCTTCACAAACTGGCCGAGTCCTGTGTCTTGG
AACTGGAACTCCAAAGACGTGGGGCCTCATCGGGATTTGGTTGGTGAATTGGGAACAGCT
CTCCGGAAGAGGAACATCCGCTATGGACTATACCACTCACTCTTAGAGTGGTTCCATCCA
CTCTATCTACTTGATAAGAAAAATGGCTTCAAAACACAGCATTTTGTCAGTGCAAAAACA
ATGCCAGAGCTGTACGACCTTGTTAACAGCTATAAACCTGATCTGATCTGGTCTGATGGG
GAGTGGGAATGTCCTGATACTTACTGGAACTCCACAAATTTTCTTTCATGGCTCTACAAT
GACAGCCCTGTCAAGGATGAGGTGGTAGTAAATGACCGATGGGGTCAGAACTGTTCCTGT
CACCATGGAGGATACTATAACTGTGAAGATAAATTCAAGCCACAGAGCTTGCCAGATCAC
AAGTGGGAGATGTGCACCAGCATTGACAAGTTTTCCTGGGGCTATCGTCGTGACATGGCA
TTGTCTGATGTTACAGAAGAATCTGAAATCATTTCGGAACTGGTTCAGACAGTAAGTTTG
GGAGGCAACTATCTTCTGAACATTGGACCAACTAAAGATGGACTGATTGTTCCCATCTTC
CAAGAAAGGCTTCTTGCTGTTGGGAAATGGCTGAGCATCAATGGGGAGGCTATCTATGCC
TCCAAACCATGGCGGGTGCAATGGGAAAAGAACACAACATCTGTATGGTATACCTCAAAG
GGATCGGCTGTTTATGCCATTTTTCTGCACTGGCCAGAAAATGGAGTCTTAAACCTTGAA
TCCCCCATAACTACCTCAACTACAAAGATAACAATGCTGGGAATTCAAGGAGATCTGAAG
TGGTCCACAGATCCAGATAAAGGTCTCTTCATCTCTCTACCCCAGTTGCCACCCTCTGCT
GTCCCCGCAGAGTTTGCTTGGACTATAAAGCTGACAGGAGTGAAGTAG

Protein sequence predicted based on nucleotide sequence

QPPRRYTPDWPSLDSRPLPAWFDEAKFGVFIHWGVFSVPAWGSEWFWWHWQGEGRPQYQR
FMRDNYPPGFSYADFGPQFTARFFHPEEWADLFQAAGAKYVVLTTKHHEGFTNWPSPVSW
NWNSKDVGPHRDLVGELGTALRKRNIRYGLYHSLLEWFHPLYLLDKKNGFKTQHFVSAKT
MPELYDLVNSYKPDLIWSDGEWECPDTYWNSTNFLSWLYNDSPVKDEVVVNDRWGQNCSC
HHGGYYNCEDKFKPQSLPDHKWEMCTSIDKFSWGYRRDMALSDVTEESEIISELVQTVSL
GGNYLLNIGPTKDGLIVPIFQERLLAVGKWLSINGEAIYASKPWRVQWEKNTTSVWYTSK
GSAVYAIFLHWPENGVLNLESPITTSTTKITMLGIQGDLKWSTDPDKGLFISLPQLPPSA
VPAEFAWTIKLTGVK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

del@1,78

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pGEn2-DEST

Synonyms:

pXLG-NtermTCMhisAviGfp-DEST

Sequencing Primer:

Forward: pGEn2-DEST F

Reverse: pGEn1-DEST R

Description:

Mammalian expression vector with a CMV promoter and N-terminal 8xHis, AviTag, and Super GFP tags; ampicillin resistance in bacteria; Gateway recombinational cloning

Comments:

None

Size (bp):

6930

Parent Vector:

None

Properties:

Gateway, acceptor (destination), fluorescent marker, mammalian expression, recombinational cloning, with tag/fusion/marker

Author Name:

University of Georgia
Kelley Moremen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

GTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCATTAGGCACCCCAGGCTTTACCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAGAGCGCCCAATACGCAAGGAAACAGCTATGACCATGTTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGGAAGGCCCATGAGGCCAGTTAATTAAGAGGTACCTTAATTAAGGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTTGTAGTTAATGATTAACCCGCCATGCTACTTATCTACGTAGCCATGCTCTAGGAAGATCGCAATTCGCCCTTAAGCTAGCTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCTGCAGAAGTTGGTCGTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCAGCGGCCGCCATGAGACTGCTGACCGCCCTGTTCGCCTACTTCATCGTGGCCCTGATCCTGGCCTTCAGCGTGTCCGCCAAGAGCATGCACCACCACCATCACCATCATCACATGAGCGGCCTGAACGACATCTTCGAGGCCCAGAAAATCGAGTGGCACGAGATGAGCAAGGGCGAGGAACTGTTCACCGGCGTGGTGCCCATCCTGGTGGAACTGGACGGCGACGTGAACGGCCACAAGTTCTCTGTGCGGGGCGAGGGCGAGGGGGACGCCACAAATGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGAAAGCTGCCCGTGCCCTGGCCTACCCTGGTCACAACCCTGACCTACGGCGTGCAGTGCTTCAGCAGATACCCCGACCACATGAAGCGGCACGATTTCTTCAAGAGCGCCATGCCCGAGGGCTACGTGCAAGAACGGACCATCAGCTTCAAGGACGACGGCACCTACAAGACCAGAGCCGAAGTGAAGTTCGAGGGCGACACCCTGGTCAACCGGATCGAGCTGAAGGGCATCGACTTCAAAGAGGACGGCAACATCCTGGGCCACAAGCTCGAGTACAACTTCAACAGCCACAACGTGTACATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGGCACAACGTGGAAGATGGCAGCGTGCAGCTGGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAATCACTACCTGAGCACCCAGAGCGTGCTGAGCAAGGACCCCAACGAGAAGCGGGACCACATGGTGCTGCTGGAATTTGTGACCGCCGCTGGCATCACCCACGGCGAATTCGCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCAGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATAGCTTGGATCCAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGAGATCTGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGACTCGAGTTAAGGGCGAATTGCCGATTAGGATCTTCCTAGAGCATGGCTACGTAGATAAGTAGCATGGCGGGTTAATCATTAACTACAAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCCTTAATTAAGAGCTCATGGCGCGCCTAGGCCTTGACGGCCTTCCGCCAATTCGCCCTATAGTGAGTCGTATTACGTCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAAACGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGAGCGCCCTGTAGCGGCCACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

Col E1 origin

1137

1804

death cassette

ccdB

ccdB death cassette (removed in plasmids with insert)

5072

5377

intron

artificial intron

2828

2960

poly-A signal

BGH pA

bovine growth hormone (BGH) polyadenylation signal

6106

6320

primer

forward

forward sequencing primer 5? AGCATGCACCACCACCATCACC 3?

3049

3070

primer

reverse

reverse sequencing primer 5' TGACAACGGGCCACAACTCCTC 3'

5729

5750

promoter

CMV

CMV promoter

2208

2790

recombination site

5'ITR

inverted terminal repeat sequence

1998

2127

recombination site

attR1

attR1 recombinational cloning site

3834

3962

recombination site

attR2

attR2 recombinational cloning site

5418

5542

recombination site

3'-ITR

inverted terminal repeat sequence

6408

6537

selectable marker

AmpR

ampicillin resistance

129

1108

selectable marker

CmR

Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)

4071

4720

signal sequence

TCM

TCM signal sequence

2977

3052

tag

His

N-terminal 8xHis tag

3055

3078

tag

AviTag

3079

3129

tag

GFP

N-terminal SuperGFP tag

3130

3825

trxn regulatory element

WPRE

woodchuck hepatitis post-transcriptional regulatory element

5558

6099

Gene

Gene Symbol :

FUCA1

Symbol Nomenclature :

FUCA1

SYNONYM :

FUCA

Designation :

alpha-L-fucosidase 1|alpha-L-fucosidase I|alpha-L-fucoside fucohydrolase 1|tissue alpha-L-fucosidase

Full Nomenclature :

fucosidase, alpha-L- 1, tissue

GENEID :

2517

Locus Tag :

RP11-45G17.1

GenBank Accession :

BC017338

HGNC :

4006

MIM :

612280

Vega :

OTTHUMG00000002965

Target GenBank:

BC017338

Reference Sequence Alignment

  

HsCD00413205      ------------------------------------------------------------
NM_000147.4       ATGCGGGCTCCGGGGATGAGGTCGCGGCCGGCGGGTCCCGCGCTGTTGCTGCTGCTGCTC
                                                                              

HsCD00413205      ---------------------------------CAGCCTCCGCGCCGCTACACCCCAGAC
NM_000147.4       TTCCTCGGAGCGGCCGAGTCGGTGCGTCGGGCCCAGCCTCCGCGCCGCTACACCCCAGAC
                                                   ***************************

HsCD00413205      TGGCCGAGCCTGGATTCTCGGCCGCTGCCGGCCTGGTTCGACGAAGCCAAGTTCGGGGTG
NM_000147.4       TGGCCGAGCCTGGATTCTCGGCCGCTGCCGGCCTGGTTCGACGAAGCCAAGTTCGGGGTG
                  ************************************************************

HsCD00413205      TTCATCCACTGGGGCGTGTTCTCGGTGCCCGCCTGGGGCAGCGAGTGGTTCTGGTGGCAC
NM_000147.4       TTCATCCACTGGGGCGTGTTCTCGGTGCCCGCCTGGGGCAGCGAGTGGTTCTGGTGGCAC
                  ************************************************************

HsCD00413205      TGGCAGGGCGAGGGGCGGCCGCAGTACCAGCGCTTCATGCGCGACAACTACCCGCCCGGC
NM_000147.4       TGGCAGGGCGAGGGGCGGCCGCAGTACCAGCGCTTCATGCGCGACAACTACCCGCCCGGC
                  ************************************************************

HsCD00413205      TTCAGCTACGCCGACTTCGGACCGCAGTTCACTGCGCGCTTCTTCCACCCGGAGGAGTGG
NM_000147.4       TTCAGCTACGCCGACTTCGGACCGCAGTTCACTGCGCGCTTCTTCCACCCGGAGGAGTGG
                  ************************************************************

HsCD00413205      GCCGACCTCTTCCAGGCCGCGGGCGCCAAGTATGTAGTTTTGACGACAAAGCATCACGAA
NM_000147.4       GCCGACCTCTTCCAGGCCGCGGGCGCCAAGTATGTAGTTTTGACGACAAAGCATCACGAA
                  ************************************************************

HsCD00413205      GGCTTCACAAACTGGCCGAGTCCTGTGTCTTGGAACTGGAACTCCAAAGACGTGGGGCCT
NM_000147.4       GGCTTCACAAACTGGCCGAGTCCTGTGTCTTGGAACTGGAACTCCAAAGACGTGGGGCCT
                  ************************************************************

HsCD00413205      CATCGGGATTTGGTTGGTGAATTGGGAACAGCTCTCCGGAAGAGGAACATCCGCTATGGA
NM_000147.4       CATCGGGATTTGGTTGGTGAATTGGGAACAGCTCTCCGGAAGAGGAACATCCGCTATGGA
                  ************************************************************

HsCD00413205      CTATACCACTCACTCTTAGAGTGGTTCCATCCACTCTATCTACTTGATAAGAAAAATGGC
NM_000147.4       CTATACCACTCACTCTTAGAGTGGTTCCATCCACTCTATCTACTTGATAAGAAAAATGGC
                  ************************************************************

HsCD00413205      TTCAAAACACAGCATTTTGTCAGTGCAAAAACAATGCCAGAGCTGTACGACCTTGTTAAC
NM_000147.4       TTCAAAACACAGCATTTTGTCAGTGCAAAAACAATGCCAGAGCTGTACGACCTTGTTAAC
                  ************************************************************

HsCD00413205      AGCTATAAACCTGATCTGATCTGGTCTGATGGGGAGTGGGAATGTCCTGATACTTACTGG
NM_000147.4       AGCTATAAACCTGATCTGATCTGGTCTGATGGGGAGTGGGAATGTCCTGATACTTACTGG
                  ************************************************************

HsCD00413205      AACTCCACAAATTTTCTTTCATGGCTCTACAATGACAGCCCTGTCAAGGATGAGGTGGTA
NM_000147.4       AACTCCACAAATTTTCTTTCATGGCTCTACAATGACAGCCCTGTCAAGGATGAGGTGGTA
                  ************************************************************

HsCD00413205      GTAAATGACCGATGGGGTCAGAACTGTTCCTGTCACCATGGAGGATACTATAACTGTGAA
NM_000147.4       GTAAATGACCGATGGGGTCAGAACTGTTCCTGTCACCATGGAGGATACTATAACTGTGAA
                  ************************************************************

HsCD00413205      GATAAATTCAAGCCACAGAGCTTGCCAGATCACAAGTGGGAGATGTGCACCAGCATTGAC
NM_000147.4       GATAAATTCAAGCCACAGAGCTTGCCAGATCACAAGTGGGAGATGTGCACCAGCATTGAC
                  ************************************************************

HsCD00413205      AAGTTTTCCTGGGGCTATCGTCGTGACATGGCATTGTCTGATGTTACAGAAGAATCTGAA
NM_000147.4       AAGTTTTCCTGGGGCTATCGTCGTGACATGGCATTGTCTGATGTTACAGAAGAATCTGAA
                  ************************************************************

HsCD00413205      ATCATTTCGGAACTGGTTCAGACAGTAAGTTTGGGAGGCAACTATCTTCTGAACATTGGA
NM_000147.4       ATCATTTCGGAACTGGTTCAGACAGTAAGTTTGGGAGGCAACTATCTTCTGAACATTGGA
                  ************************************************************

HsCD00413205      CCAACTAAAGATGGACTGATTGTTCCCATCTTCCAAGAAAGGCTTCTTGCTGTTGGGAAA
NM_000147.4       CCAACTAAAGATGGACTGATTGTTCCCATCTTCCAAGAAAGGCTTCTTGCTGTTGGGAAA
                  ************************************************************

HsCD00413205      TGGCTGAGCATCAATGGGGAGGCTATCTATGCCTCCAAACCATGGCGGGTGCAATGGGAA
NM_000147.4       TGGCTGAGCATCAATGGGGAGGCTATCTATGCCTCCAAACCATGGCGGGTGCAATGGGAA
                  ************************************************************

HsCD00413205      AAGAACACAACATCTGTATGGTATACCTCAAAGGGATCGGCTGTTTATGCCATTTTTCTG
NM_000147.4       AAGAACACAACATCTGTATGGTATACCTCAAAGGGATCGGCTGTTTATGCCATTTTTCTG
                  ************************************************************

HsCD00413205      CACTGGCCAGAAAATGGAGTCTTAAACCTTGAATCCCCCATAACTACCTCAACTACAAAG
NM_000147.4       CACTGGCCAGAAAATGGAGTCTTAAACCTTGAATCCCCCATAACTACCTCAACTACAAAG
                  ************************************************************

HsCD00413205      ATAACAATGCTGGGAATTCAAGGAGATCTGAAGTGGTCCACAGATCCAGATAAAGGTCTC
NM_000147.4       ATAACAATGCTGGGAATTCAAGGAGATCTGAAGTGGTCCACAGATCCAGATAAAGGTCTC
                  ************************************************************

HsCD00413205      TTCATCTCTCTACCCCAGTTGCCACCCTCTGCTGTCCCCGCAGAGTTTGCTTGGACTATA
NM_000147.4       TTCATCTCTCTACCCCAGTTGCCACCCTCTGCTGTCCCCGCAGAGTTTGCTTGGACTATA
                  ************************************************************

HsCD00413205      AAGCTGACAGGAGTGAAGTAG
NM_000147.4       AAGCTGACAGGAGTGAAGTAA
                  ********************.