Gene:
Gene Symbol:
GUSB
Gene Name:
glucuronidase, beta
Original Clone ID:
HGH02_M01
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEn1-DEST
Format:
FUSION
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00413288
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
CTGCAGGGCGGGATGCTGTACCCCCAGGAGAGCCCGTCGCGGGAGTGCAAGGAGCTGGAC
GGCCTCTGGAGCTTCCGCGCCGACTTCTCTGACAACCGACGCCGGGGCTTCGAGGAGCAG
TGGTACCGGCGGCCGCTGTGGGAGTCAGGCCCCACCGTGGACATGCCAGTTCCCTCCAGC
TTCAATGACATCAGCCAGGACTGGCGTCTGCGGCATTTTGTCGGCTGGGTGTGGTACGAA
CGGGAGGTGATCCTGCCGGAGCGATGGACCCAGGACCTGCGCACAAGAGTGGTGCTGAGG
ATTGGCAGTGCCCATTCCTATGCCATCGTGTGGGTGAATGGGGTCGACACGCTAGAGCAT
GAGGGGGGCTACCTCCCCTTCGAGGCCGACATCAGCAACCTGGTCCAGGTGGGGCCCCTG
CCCTCCCGGCTCCGAATCACTATCGCCATCAACAACACACTCACCCCCACCACCCTGCCA
CCAGGGACCATCCAATACCTGACTGACACCTCCAAGTATCCCAAGGGTTACTTTGTCCAG
AACACATATTTTGACTTTTTCAACTACGCTGGACTGCAGCGGTCTGTACTTCTGTACACG
ACACCCACCACCTACATCGATGACATCACCGTCACCACCAGCGTGGAGCAAGACAGTGGG
CTGGTGAATTACCAGATCTCTGTCAAGGGCAGTAACCTGTTCAAGTTGGAAGTGCGTCTT
TTGGATGCAGAAAACAAAGTCGTGGCGAATGGGACTGGGACCCAGGGCCAACTTAAGGTG
CCAGGTGTCAGCCTCTGGTGGCCGTACCTGATGCACGAACGCCCTGCCTATCTGTATTCA
TTGGAGGTGCAGCTGACTGCACAGACGTCACTGGGGCCTGTGTCTGACTTCTACACACTC
CCTGTGGGGATCCGCACTGTGGCTGTCACCAAGAGCCAGTTCCTCATCAATGGGAAACCT
TTCTATTTCCACGGTGTCAACAAGCATGAGGATGCGGACATCCGAGGGAAGGGCTTCGAC
TGGCCGCTGCTGGTGAAGGACTTCAACCTGCTTCGCTGGCTTGGTGCCAACGCTTTCCGT
ACCAGCCACTACCCCTATGCAGAGGAAGTGATGCAGATGTGTGACCGCTATGGGATTGTG
GTCATCGATGAGTGTCCCGGCGTGGGCCTGGCGCTGCCGCAGTTCTTCAACAACGTTTCT
CTGCATCACCACATGCAGGTGATGGAAGAAGTGGTGCGTAGGGACAAGAACCACCCCGCG
GTCGTGATGTGGTCTGTGGCCAACGAGCCTGCGTCCCACCTAGAATCTGCTGGCTACTAC
TTGAAGATGGTGATCGCTCACACCAAATCCTTGGACCCCTCCCGGCCTGTGACCTTTGTG
AGCAACTCTAACTATGCAGCAGACAAGGGGGCTCCGTATGTGGATGTGATCTGTTTGAAC
AGCTACTACTCTTGGTATCACGACTACGGGCACCTGGAGTTGATTCAGCTGCAGCTGGCC
ACCCAGTTTGAGAACTGGTATAAGAAGTATCAGAAGCCCATTATTCAGAGCGAGTATGGA
GCAGAAACGATTGCAGGGTTTCACCAGGATCCACCTCTGATGTTCACTGAAGAGTACCAG
AAAAGTCTGCTAGAGCAGTACCATCTGGGTCTGGATCAAAAACGCAGAAAATACGTGGTT
GGAGAGCTCATTTGGAATTTTGCCGATTTCATGACTGAACAGTCACCGACGAGAGTGCTG
GGGAATAAAAAGGGGATCTTCACTCGGCAGAGACAACCAAAAAGTGCAGCGTTCCTTTTG
CGAGAGAGATACTGGAAGATTGCCAATGAAACCAGGTATCCCCACTCAGTAGCCAAGTCA
CAATGTTTGGAAAACAGCCTGTTTACT
Protein sequence predicted based on nucleotide sequence
LQGGMLYPQESPSRECKELDGLWSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSS FNDISQDWRLRHFVGWVWYEREVILPERWTQDLRTRVVLRIGSAHSYAIVWVNGVDTLEH EGGYLPFEADISNLVQVGPLPSRLRITIAINNTLTPTTLPPGTIQYLTDTSKYPKGYFVQ NTYFDFFNYAGLQRSVLLYTTPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRL LDAENKVVANGTGTQGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTL PVGIRTVAVTKSQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFR TSHYPYAEEVMQMCDRYGIVVIDECPGVGLALPQFFNNVSLHHHMQVMEEVVRRDKNHPA VVMWSVANEPASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLN SYYSWYHDYGHLELIQLQLATQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQ KSLLEQYHLGLDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLL RERYWKIANETRYPHSVAKSQCLENSLFT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,66
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGEn1-DEST
Synonyms:
pXLG-NtermTCMhisStrep-DEST
Sequencing Primer:
Forward:
pGEn3-DEST-5'F
Reverse:
pGEn1-DEST R
Description:
Mammalian expression vector with a CMV promoter and N-terminal TCM 8xHis and Strep tag; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments:
None
Size (bp):
6210
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
Col E1 origin
1137
1804
death cassette
ccdB
ccdB death cassette (removed in plasmids with insert)
4352
4657
intron
artificial intron
Artificial Intron
2828
2960
poly-A signal
BGH pA
bovine growth hormone (BGH) polyadenylation signal
5386
5600
primer
forward
forward primer 5? AGCATGCACCACCACCATCACC 3?
3049
3070
primer
reverse
reverse primer 5' TGACAACGGGCCACAACTCCTC 3'
5009
5030
promoter
CMV
CMV mammalian promoter
2208
2790
recombination site
5'ITR
inverted terminal repeat sequence
1998
2127
recombination site
attR1
attR1 recombinational cloning site
3114
3242
recombination site
attR2
attR2 recombinational cloning site
4698
4822
recombination site
3'-ITR
inverted terminal repeat sequence
5688
5817
selectable marker
AmpR
ampicillin resistance gene
129
1108
selectable marker
CmR
Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)
3351
4000
signal sequence
TCM
TCM signal sequence
2977
3054
tag
His
8xHis tag
3055
3078
tag
strep
N-terminal Strep tag
3085
3108
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
4838
5379
