Gene:
Gene Symbol:
HEXA
Gene Name:
hexosaminidase A (alpha polypeptide)
Original Clone ID:
HGH20_M01
Keyword:
partial cds (N-terminal deletion)
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGEn1-DEST
Format:
CLOSED
Source:
Glyco-enzyme clone collection
Description:
All clones in the PSI Materials Repository related to human and bacterial glycosylation enzymes (Glyco-enzyme clone collection) were generated through the support of NIH grant RR005351. All users of these clones are requested to cite this grant source on all publications employing these resources
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Georgia
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
Center for Personalized Diagnostics
Arizona State University
Kelley Moremen
HsCD00413319
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
CTCTGGCCCTGGCCTCAGAACTTCCAAACCTCCGACCAGCGCTACGTCCTTTACCCGAAC
AACTTTCAATTCCAGTACGATGTCAGCTCGGCCGCGCAGCCCGGCTGCTCAGTCCTCGAC
GAGGCCTTCCAGCGCTATCGTGACCTGCTTTTCGGTTCCGGGTCTTGGCCCCGTCCTTAC
CTCACAGGGAAACGGCATACACTGGAGAAGAATGTGTTGGTTGTCTCTGTAGTCACACCT
GGATGTAACCAGCTTCCTACTTTGGAGTCAGTGGAGAATTATACCCTGACCATAAATGAT
GACCAGTGTTTACTCCTCTCTGAGACTGTCTGGGGAGCTCTCCGAGGTCTGGAGACTTTT
AGCCAGCTTGTTTGGAAATCTGCTGAGGGCACATTCTTTATCAACAAGACTGAGATTGAG
GACTTTCCCCGCTTTCCTCACCGGGGCTTGCTGTTGGATACATCTCGCCATTACCTGCCA
CTCTCTAGCATCCTGGACACTCTGGATGTCATGGCGTACAATAAATTGAACGTGTTCCAC
TGGCATCTGGTAGATGATCCTTCCTTCCCATATGAGAGCTTCACTTTTCCAGAGCTCATG
AGAAAGGGGTCCTACAACCCTGTCACCCACATCTACACAGCACAGGATGTGAAGGAGGTC
ATTGAATACGCACGGCTCCGGGGTATCCGTGTGCTTGCAGAGTTTGACACTCCTGGCCAC
ACTTTGTCCTGGGGACCAGGTATCCCTGGATTACTGACTCCTTGCTACTCTGGGTCTGAG
CCCTCTGGCACCTTTGGACCAGTGAATCCCAGTCTCAATAATACCTATGAGTTCATGAGC
ACATTCTTCTTAGAAGTCAGCTCTGTCTTCCCAGATTTTTATCTTCATCTTGGAGGAGAT
GAGGTTGATTTCACCTGCTGGAAGTCCAACCCAGAGATCCAGGACTTTATGAGGAAGAAA
GGCTTCGGTGAGGACTTCAAGCAGCTGGAGTCCTTCTACATCCAGACGCTGCTGGACATC
GTCTCTTCTTATGGCAAGGGCTATGTGGTGTGGCAGGAGGTGTTTGATAATAAAGTAAAG
ATTCAGCCAGACACAATCATACAGGTGTGGCGAGAGGATATTCCAGTGAACTATATGAAG
GAGCTGGAACTGGTCACCAAGGCCGGCTTCCGGGCCCTTCTCTCTGCCCCCTGGTACCTG
AACCGTATATCCTATGGCCCTGACTGGAAGGATTTCTACGTAGTGGAACCCCTGGCATTT
GAAGGTACCCCTGAGCAGAAGGCTCTGGTGATTGGTGGAGAGGCTTGTATGTGGGGAGAA
TATGTGGACAACACAAACCTGGTCCCCAGGCTCTGGCCCAGAGCAGGGGCTGTTGCCGAA
AGGCTGTGGAGCAACAAGTTGACATCTGACCTGACATTTGCCTATGAACGTTTGTCACAC
TTCCGCTGTGAGTTGCTGAGGCGAGGTGTCCAGGCCCAACCCCTCAATGTAGGCTTCTGT
GAGCAGGAGTTTGAACAGACCTAG
Protein sequence predicted based on nucleotide sequence
LWPWPQNFQTSDQRYVLYPNNFQFQYDVSSAAQPGCSVLDEAFQRYRDLLFGSGSWPRPY LTGKRHTLEKNVLVVSVVTPGCNQLPTLESVENYTLTINDDQCLLLSETVWGALRGLETF SQLVWKSAEGTFFINKTEIEDFPRFPHRGLLLDTSRHYLPLSSILDTLDVMAYNKLNVFH WHLVDDPSFPYESFTFPELMRKGSYNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGH TLSWGPGIPGLLTPCYSGSEPSGTFGPVNPSLNNTYEFMSTFFLEVSSVFPDFYLHLGGD EVDFTCWKSNPEIQDFMRKKGFGEDFKQLESFYIQTLLDIVSSYGKGYVVWQEVFDNKVK IQPDTIIQVWREDIPVNYMKELELVTKAGFRALLSAPWYLNRISYGPDWKDFYVVEPLAF EGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAVAERLWSNKLTSDLTFAYERLSH FRCELLRRGVQAQPLNVGFCEQEFEQT*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
del@1,66
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGEn1-DEST
Synonyms:
pXLG-NtermTCMhisStrep-DEST
Sequencing Primer:
Forward:
pGEn3-DEST-5'F
Reverse:
pGEn1-DEST R
Description:
Mammalian expression vector with a CMV promoter and N-terminal TCM 8xHis and Strep tag; ampicillin resistance in bacteria; Gateway recombinational cloning
Comments:
None
Size (bp):
6210
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
Col E1 origin
1137
1804
death cassette
ccdB
ccdB death cassette (removed in plasmids with insert)
4352
4657
intron
artificial intron
Artificial Intron
2828
2960
poly-A signal
BGH pA
bovine growth hormone (BGH) polyadenylation signal
5386
5600
primer
forward
forward primer 5? AGCATGCACCACCACCATCACC 3?
3049
3070
primer
reverse
reverse primer 5' TGACAACGGGCCACAACTCCTC 3'
5009
5030
promoter
CMV
CMV mammalian promoter
2208
2790
recombination site
5'ITR
inverted terminal repeat sequence
1998
2127
recombination site
attR1
attR1 recombinational cloning site
3114
3242
recombination site
attR2
attR2 recombinational cloning site
4698
4822
recombination site
3'-ITR
inverted terminal repeat sequence
5688
5817
selectable marker
AmpR
ampicillin resistance gene
129
1108
selectable marker
CmR
Chloramphenicol resistance (CmR) gene (removed in plasmids with insert)
3351
4000
signal sequence
TCM
TCM signal sequence
2977
3054
tag
His
8xHis tag
3055
3078
tag
strep
N-terminal Strep tag
3085
3108
trxn regulatory element
WPRE
woodchuck hepatitis post-transcriptional regulatory element
4838
5379
