Gene:
Gene Symbol:
Sav1
Gene Name:
salvador homolog 1 (Drosophila)
Original Clone ID:
939.1.32_GO.34288
Keyword:
full-length cds
Species:
Mus musculus
Type:
cDNA
Vector Name:
pVP16
Format:
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
PSI
Center for Eukaryotic Structural Genomics
MmCD00422566
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1179nts
Open reading frame: 51 to 1229
GGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCCTGTCCCGCAAGAAAACCAAAAACGAGGTGTCTAAGCCGGCCGAGGTGC
AGGGCAAGTACGTGAAGAAGGAGACGTCGCCCCTGCTGCGGAATCTCATGCCTTCATTCA
TTCGGCACGGTCCAACAATTCCCAGACGGACTGACCTCTGTCTTCCAGATTCAAGTGCTA
CTGCTTTCTCAGCTTCTGGAGACGGTATAGTTTCAAGAAACCAGAGTTTCCTGAGAACTG
CAATTCAAAGGACACCTCATGAAGTAATGAGAAGAGAAAGCCACAGACTGTCTGCCCCTT
CTTACCTTGTCAGGAGCCTAGCAGATGTCCCTCGAGAGTGTGGCTCATCACAGTCATTTT
TGACAGAAGTTAACTTTGCTGTTGAGAATGGAGACTCTGGCTCCCGATACTTCTTCTCAG
ATAACTTTTTTGATGGACAGAGAAGGCGGCCACTTGGAGATCGTGCACAAGAAGATTACA
GATATTATGAATACAACCATGATCTCTTCCAGAGGATGCCACAGAGTCAGGGGAGGCACA
CTTCAGGTATTGGGAGAGTCACGGCTACATCTCTAGGGAATTTAACTAACCATGGATCTG
AAGATTTACCCCTTCCTCCTGGCTGGTCTGTGGACTGGACAATGAGAGGGAGAAAATACT
ACATAGATCATAACACAAATACCACTCACTGGAGTCATCCCCTTGAACGAGAAGGACTTC
CTCCTGGCTGGGAACGAGTAGAGTCATCAGAATTTGGAACCTATTACGTGGATCACACCA
ATAAAAGGGCTCAGTACAGGCACCCCTGTGCTCCGAGTGTACCTCGGTATGATCAGCCTC
CACCCATCACGTATCAGCCACAACAAACTGAAAGAAATCAGTCTCTCCTGGTCCCTGCAA
ATCCCTACCATACTGCAGAAATTCCTGACTGGCTTCAGGTTTATGCCCGAGCCCCTGTGA
AATATGACCACATTCTGAAGTGGGAGCTCTTCCAGCTGGCTGACCTGGACACGTACCAGG
GAATGCTGAAGTTGCTCTTCATGAAGGAACTGGAGCAGATTGTGAAGTTGTACGAGGCCT
ACAGACAGGCTCTTCTCACTGAGTTGGAAAACCGCAAGCAGAGGCAGCAGTGGTATGCCC
AGCAGCATGGCAAGACGTTCTTAAGTTAATAGGACCCAGCTTTCT
Protein sequence predicted based on nucleotide sequence
ENLYFQSLSRKKTKNEVSKPAEVQGKYVKKETSPLLRNLMPSFIRHGPTIPRRTDLCLPD SSATAFSASGDGIVSRNQSFLRTAIQRTPHEVMRRESHRLSAPSYLVRSLADVPRECGSS QSFLTEVNFAVENGDSGSRYFFSDNFFDGQRRRPLGDRAQEDYRYYEYNHDLFQRMPQSQ GRHTSGIGRVTATSLGNLTNHGSEDLPLPPGWSVDWTMRGRKYYIDHNTNTTHWSHPLER EGLPPGWERVESSEFGTYYVDHTNKRAQYRHPCAPSVPRYDQPPPITYQPQQTERNQSLL VPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDTYQGMLKLLFMKELEQIVKL YEAYRQALLTELENRKQRQQWYAQQHGKTFLS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in GO.34288
Recommended expression in:
Not Applicable
Vector Name:
pVP16
Synonyms:
''
Sequencing Primer:
Forward:
MBP Forward
Reverse:
pQE Reverse
Description:
Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.
Comments:
TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette
Size (bp):
7631
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
Center for Eukaryotic Structural Genomics
Publications:
PMID:
15750721
Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
6471
5789
negative selection marker
ccdB
ccdB death cassette gene (lost in with-insert form)
2643
2852
promoter
T5
T5 promoter
5
54
recombination site
AttR1
AttR1 recombination site
1316
1440
recombination site
AttR2
AttR2 recombination site
3020
2896
repressor protein gene
LacI
LacI coding region
4132
5211
selectable marker
CmR
chloramphenicol resistance gene (CmR) (Lost in with-insert form)
1549
2205
selectable marker
AmpR
ampicillin resistance gene
7228
6569
tag
MBP
N-terminal MBP
148
1246
tag
His
N-terminal 8xHis tag
128
145
