DNASU Plasmid | CORO6 (Homo sapiens) in pVP16

Explanation of Terms

Gene:

Gene Symbol: CORO6

Gene Name: coronin 6

Original Clone ID:

1141.1.08_GO.37222

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pVP16

Format:

CLOSED

Source:

Center for Eukaryotic Structural Genomics

Description:

None

Comments:

Linker not verified. Low quality silent and/or missense mutations found in CDS.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics

HsCD00422619

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 732nts

Open reading frame: 51 to 782

Reference Sequence Alignment

GCTTCGAGGAAAACCTGNACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGN
ACTTCCAGTCCCCGATTCCCTGGGGGTGGTTTGCTGTGACTGCATGCGGCGCGCAGCGGA
ACAACTTCGAGGAGCCAGTGGCACTGCAGGAGATGGACACAAGCAACGGGGTCCTATTGC
CCTTTTACGATCCCGACTCCAGCATCGTCTACCTGTGTGGCAAGGGCGACAGCAGCATTC
GGTACTTTGAGATTACCGACGAGCCGCCTTTCGTGCACTACCTGAACACGTTCAGCAGCA
AAGAGCCGCAGCGGGGCATGGGTTTCATGCCCAAAAGGGGACTGGATGTCAGCAAGTGTG
AGATCGCCCGGTTCTACAAGCTACACGAAAGAAAGTGTGAACCTATCATCATGACTGTGC
CCCGCAAGTCAGACCTCTTCCAGGACGATCTGTACCCGGATACGCCAGGCCCGGAGCCGG
CCCTAGAAGCGGACGAATGGCTATCCGGCCAGGACGCCGAACCCGTGCTCATTTCGCTGA
GGGACGGCTATGTGCCCCCCAAGCACCGCGAGCTCCGGGTCACGAAGCGCAACATCCTGG
ACGTGCGCCCGCCCTCCGGCCCCCGCCGCAGCCAGTCGGCCAGCGACGCCCCCTTGTCGC
AGCACACCCTGGAGACGCTGCTGGAAGAGATCAAGGCCCTCCGCGAGCGGGTGCAGGCCC
AGGAGCAGCGCATCACGGCTCTGGAGAACATGCTGTGCGAGCTGGTGGACGGCACGGACT
AGTAGGACCCAGCTTTCTTGTACAAAG

Protein sequence predicted based on nucleotide sequence

ENLFQSPIPWGWFAVTACGAQRNNFEEPVALQEMDTSNGVLLPFYDPDSSIVYLCGKGDS
SIRYFEITDEPPFVHYLNTFSSKEPQRGMGFMPKRGLDVSKCEIARFYKLHERKCEPIIM
TVPRKSDLFQDDLYPDTPGPEPALEADEWLSGQDAEPVLISLRDGYVPPKHRELRVTKRN
ILDVRPPSGPRRSQSASDAPLSQHTLETLLEEIKALRERVQAQEQRITALENMLCELVDG
TD*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

t10n;g26t,S9I

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Tested_Not_Purified

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in GO.37222

Recommended expression in:

Not Applicable

Vector Name:

pVP16

Synonyms:

Sequencing Primer:

Forward: MBP Forward

Reverse: pQE Reverse

Description:

Bacterial (E. coli) expression, adds N-terminal 8xHis and MBP, adds TEV site on 5p end of insert; ampicillin resistance in bacteria; recombinational cloning; Gateway cloning into this vector will remove the Gateway ccdB death casette.

Comments:

TEV protease cleavage site is "partial" and not functional. The TEV cleavage site must be added to the insert by PCR, which allows amino acids encoded by the attB1 site to be cut off after protein expression. Gateway cloning into this vector will remove the Gateway cassette

Size (bp):

7631

Parent Vector:

None

Properties:

Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker

Author Name:

Center for Eukaryotic Structural Genomics

Publications:

PMID: 15750721

Title: Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination

Vector Map:

Vector Sequence:

Sequence:

CCTCGAGAAATCATAAAAAATTTATTTGCTTTGTGAGCGGATAACAATTATAATAGATTCAATTGTGAGCGGATAACAATTTCACACAGAATTCATTAAAGAGGAGAAATTAACCATGGGACATCACCATCATCACCATCACCATTCAAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACTAATTCGAGCTCGAACAACAACAACAATAACAATAACAACAACCTCGGGATCGACGAAAACCTGTACCTCACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTTCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTATGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATGGCGCGCCTGTAGGACGTCGACGGTACCATCGATACGCGTTCGAAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGCTTGGCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCCTCTAGATTACGTGCAGTCGATGATAAGCTGTCAAACATGAGAATTGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCGGAATTTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCTAGAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAC

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

ColE1-type bacterial origin of replication

6471

5789

negative selection marker

ccdB

ccdB death cassette gene (lost in with-insert form)

2643

2852

promoter

T5 promoter

recombination site

AttR1

AttR1 recombination site

1316

1440

recombination site

AttR2

AttR2 recombination site

3020

2896

repressor protein gene

LacI

LacI coding region

4132

5211

selectable marker

CmR

chloramphenicol resistance gene (CmR) (Lost in with-insert form)

1549

2205

selectable marker

AmpR

ampicillin resistance gene

7228

6569

tag

MBP

N-terminal MBP

148

1246

tag

His

N-terminal 8xHis tag

128

145

Gene

Gene Symbol :

CORO6

Symbol Nomenclature :

CORO6

Designation :

clipin-E|coronin, actin binding protein 6|coronin-6|coronin-like protein E

Full Nomenclature :

coronin 6

GENEID :

84940

Locus Tag :

PP1009

GI :

40352764

GenBank Accession :

BC064514

HGNC :

21356

Vega :

OTTHUMG00000132732

Target GenBank:

BC064514

Reference Sequence Alignment

  

HsCD00422619        ------------------------------------------------------------
NM_032854.3         ATGAGCAGACGTGTGGTTCGGCAAAGCAAGTTCCGCCATGTGTTTGGGCAGGCAGCAAAG
XM_005258051.1      ATGAGCAGACGTGTGGTTCGGCAAAGCAAGTTCCGCCATGTGTTTGGGCAGGCAGCAAAG
XM_005258047.1      ATGAGCAGACGTGTGGTTCGGCAAAGCAAGTTCCGCCATGTGTTTGGGCAGGCAGCAAAG
XM_005258049.1      ATGAGCAGACGTGTGGTTCGGCAAAGCAAGTTCCGCCATGTGTTTGGGCAGGCAGCAAAG
                                                                                

HsCD00422619        ------------------------------------------------------------
NM_032854.3         GCCGACCAGGCCTACGAGGACATCCGTGTGTCCAAGGTCACATGGGACAGCTCCTTCTGT
XM_005258051.1      GCCGACCAGGCCTACGAGGACATCCGTGTGTCCAAGGTCACATGGGACAGCTCCTTCTGT
XM_005258047.1      GCCGACCAGGCCTACGAGGACATCCGTGTGTCCAAGGTCACATGGGACAGCTCCTTCTGT
XM_005258049.1      GCCGACCAGGCCTACGAGGACATCCGTGTGTCCAAGGTCACATGGGACAGCTCCTTCTGT
                                                                                

HsCD00422619        ------------------------------------------------------------
NM_032854.3         GCCGTCAACCCCAAATTCCTGGCCATTATTGTGGAGGCTGGAGGCGGGGGTGCCTTCATC
XM_005258051.1      GCCGTCAACCCCAAATTCCTGGCCATTATTGTGGAGGCTGGAGGCGGGGGTGCCTTCATC
XM_005258047.1      GCCGTCAACCCCAAATTCCTGGCCATTATTGTGGAGGCTGGAGGCGGGGGTGCCTTCATC
XM_005258049.1      GCCGTCAACCCCAAATTCCTGGCCATTATTGTGGAGGCTGGAGGCGGGGGTGCCTTCATC
                                                                                

HsCD00422619        ------------------------------------------------------------
NM_032854.3         GTCCTGCCTCTGGCCAAGACAGGGCGAGTGGATAAGAACTACCCACTGGTCACTGGGCAC
XM_005258051.1      GTCCTGCCTCTGGCCAAGGTG---------------------------------------
XM_005258047.1      GTCCTGCCTCTGGCCAAGACAGGGCGAGTGGATAAGAACTACCCACTGGTCACTGGGCAC
XM_005258049.1      GTCCTGCCTCTGGCCAAGACAGGGCGAGTGGATAAGAACTACCCACTGGTCACTGGGCAC
                                                                                

HsCD00422619        ------------------------------------------------------------
NM_032854.3         ACTGCCCCTGTGCTGGATATTGACTGGTGTCCACACAATGACAACGTTATCGCCAGTGCC
XM_005258051.1      ------------------------------------------------------------
XM_005258047.1      ACTGCCCCTGTGCTGGATATTGACTGGTGTCCACACAATGACAACGTTATCGCCAGTGCC
XM_005258049.1      ACTGCCCCTGTGCTGGATATTGACTGGTGTCCACACAATGACAACGTTATCGCCAGTGCC
                                                                                

HsCD00422619        ------------------------------------------------------------
NM_032854.3         TCAGACGACACCACCATCATGGTGTGGCAGATTCCAGACTATACCCCCATGCGCAACATT
XM_005258051.1      ------------------------TGGCAGATTCCAGACTATACCCCCATGCGCAACATT
XM_005258047.1      TCAGACGACACCACCATCATGGTGTGGCAGATTCCAGACTATACCCCCATGCGCAACATT
XM_005258049.1      TCAGACGACACCACCATCATGGTGTGGCAGATTCCAGACTATACCCCCATGCGCAACATT
                                                                                

HsCD00422619        ------------------------------------------------------------
NM_032854.3         ACGGAACCTATCATCACACTTGAGGGCCACTCCAAGCGTGTGGGCATCCTCTCCTGGCAC
XM_005258051.1      ACGGAACCTATCATCACACTTGAGGGCCACTCCAAGCGTGTGGGCATCCTCTCCTGGCAC
XM_005258047.1      ACGGAACCTATCATCACACTTGAGGGCCACTCCAAGCGTGTGGGCATCCTCTCCTGGCAC
XM_005258049.1      ACGGAACCTATCATCACACTTGAGGGCCACTCCAAGCGTGTGGGCATCCTCTCCTGGCAC
                                                                                

HsCD00422619        ------------------------------------------------------------
NM_032854.3         CCTACTGCCAGGAATGTCCTGCTCAGTGCAGGTGGTGACAATGTGATCATCATCTGGAAT
XM_005258051.1      CCTACTGCCAGGAATGTCCTGCTCAGTGCAGGTGGTGACAATGTGATCATCATCTGGAAT
XM_005258047.1      CCTACTGCCAGGAATGTCCTGCTCAGTGCAGGTGGTGACAATGTGATCATCATCTGGAAT
XM_005258049.1      CCTACTGCCAGGAATGTCCTGCTCAGTGCAGGTGGTGACAATGTGATCATCATCTGGAAT
                                                                                

HsCD00422619        ----------------------------------------------------GCTTCGAG
NM_032854.3         GTGGGCACCGGGGAGGTGCTGCTGAGCCTGGATGATATGCACCCAGACGTCATCCACAGT
XM_005258051.1      GTGGGCACCGGGGAGGTGCTGCTGAGCCTGGATGATATGCACCCAGACGTCATCCACAGT
XM_005258047.1      GTGGGCACCGGGGAGGTGCTGCTGAGCCTGGATGATATGCACCCAGACGTCATCCACAGT
XM_005258049.1      GTGGGCACCGGGGAGGTGCTGCTGAGCCTGGATGATATGCACCCAGACGTCATCCACAGT
                                                                         * :*.. 

HsCD00422619        GAAAACCTGNACCTCACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGNACTTCCAG
NM_032854.3         GTGTGCTGGAACAGCAACGGTAGCCTGCTAGCCACCACCTGCAAGGACAAGACCTTGCGC
XM_005258051.1      GTGTGCTGGAACAGCAACGGTAGCCTGCTAGCCACCACCTGCAAGGACAAGACCTTGCGC
XM_005258047.1      GTGTGCTGGAACAGCAACGGTAGCCTGCTAGCCACCACCTGCAAGGACAAGACCTTGCGC
XM_005258049.1      GTGTGCTGGAACAGCAACGGTAGCCTGCTAGCCACCACCTGCAAGGACAAGACCTTGCGC
                    *:.:.*  * **. **...**:   : .:*....*.. ** *.*..**.:* .*** *. 

HsCD00422619        TCCCCGATTCCCTGGGGGTGGTTTGCTGTGACTGCATGCGGCGCG---------------
NM_032854.3         ATCATTGACCCCAGAAAAGGCCAAGTGGTGGCGGAGAGGTTTGCGGCCCACGAGGGGATG
XM_005258051.1      ATCATTGACCCCAGAAAAGGCCAAGTGGTGGCGGAGAGGTTTGCGGCCCACGAGGGGATG
XM_005258047.1      ATCATTGACCCCAGAAAAGGCCAAGTGGTGGCGGAGAGGTTTGCGGCCCACGAGGGGATG
XM_005258049.1      ATCATTGACCCCAGAAAAGGCCAAGTGGTGGCGGAGAGGTTTGCGGCCCACGAGGGGATG
                    : *.  .: ***:*.... *  ::*  ***.* *..:*    ***               

HsCD00422619        ------------------------------------------------------------
NM_032854.3         AGGCCCATGCGGGCCGTCTTCACGCGCCAGGGCCATATCTTCACCACGGGCTTCACCCGC
XM_005258051.1      AGGCCCATGCGGGCCGTCTTCACGCGCCAGGGCCATATCTTCACCACGGGCTTCACCCGC
XM_005258047.1      AGGCCCATGCGGGCCGTCTTCACGCGCCAGGGCCATATCTTCACCACGGGCTTCACCCGC
XM_005258049.1      AGGCCCATGCGGGCCGTCTTCACGCGCCAGGGCCATATCTTCACCACGGGCTTCACCCGC
                                                                                

HsCD00422619        ---------------------------CAGCGGAACAACTTCGAGGAGCCAGTGGCACTG
NM_032854.3         ATGAGCCAGCGAGAGCTGGGCCTGTGGGACCCGAACAACTTCGAGGAGCCAGTGGCACTG
XM_005258051.1      ATGAGCCAGCGAGAGCTGGGCCTGTGGGACCCGAACAACTTCGAGGAGCCAGTGGCACTG
XM_005258047.1      ATGAGCCAGCGAGAGCTGGGCCTGTGGGACCCGAACAACTTCGAGGAGCCAGTGGCACTG
XM_005258049.1      ATGAGCCAGCGAGAGCTGGGCCTGTGGGACCCGAACAACTTCGAGGAGCCAGTGGCACTG
                                                * * ****************************

HsCD00422619        CAGGAGATGGACACAAGCAACGGGGTCCTATTGCCCTTTTACGATCCCGACTCCAGCATC
NM_032854.3         CAGGAGATGGACACAAGCAACGGGGTCCTATTGCCCTTTTACGATCCCGACTCCAGCATC
XM_005258051.1      CAGGAGATGGACACAAGCAACGGGGTCCTATTGCCCTTTTACGATCCCGACTCCAGCATC
XM_005258047.1      CAGGAGATGGACACAAGCAACGGGGTCCTATTGCCCTTTTACGATCCCGACTCCAGCATC
XM_005258049.1      CAGGAGATGGACACAAGCAACGGGGTCCTATTGCCCTTTTACGATCCCGACTCCAGCATC
                    ************************************************************

HsCD00422619        GTCTACCTGTGTGGCAAGGGCGACAGCAGCATTCGGTACTTTGAGATTACCGACGAGCCG
NM_032854.3         GTCTACCTGTGTGGCAAGGGCGACAGCAGCATTCGGTACTTTGAGATTACCGACGAGCCG
XM_005258051.1      GTCTACCTGTGTGGCAAGGGCGACAGCAGCATTCGGTACTTTGAGATTACCGACGAGCCG
XM_005258047.1      GTCTACCTGTGTGGCAAGGGCGACAGCAGCATTCGGTACTTTGAGATTACCGACGAGCCG
XM_005258049.1      GTCTACCTGTGTGGCAAGGGCGACAGCAGCATTCGGTACTTTGAGATTACCGACGAGCCG
                    ************************************************************

HsCD00422619        CCTTTCGTGCACTACCTGAACACGTTCAGCAGCAAAGAGCCGCAGCGGGGCATGGGTTTC
NM_032854.3         CCTTTCGTGCACTACCTGAACACGTTCAGCAGCAAAGAGCCGCAGCGGGGCATGGGTTTC
XM_005258051.1      CCTTTCGTGCACTACCTGAACACGTTCAGCAGCAAAGAGCCGCAGCGGGGCATGGGTTTC
XM_005258047.1      CCTTTCGTGCACTACCTGAACACGTTCAGCAGCAAAGAGCCGCAGCGGGGCATGGGTTTC
XM_005258049.1      CCTTTCGTGCACTACCTGAACACGTTCAGCAGCAAAGAGCCGCAGCGGGGCATGGGTTTC
                    ************************************************************

HsCD00422619        ATGCCCAAAAGGGGACTGGATGTCAGCAAGTGTGAGATCGCCCGGTTCTACAAGCTACAC
NM_032854.3         ATGCCCAAAAGGGGACTGGATGTCAGCAAGTGTGAGATCGCCCGGTTCTACAAGCTACAC
XM_005258051.1      ATGCCCAAAAGGGGACTGGATGTCAGCAAGTGTGAGATCGCCCGGTTCTACAAGCTACAC
XM_005258047.1      ATGCCCAAAAGGGGACTGGATGTCAGCAAGTGTGAGATCGCCCGGTTCTACAAGCTACAC
XM_005258049.1      ATGCCCAAAAGGGGACTGGATGTCAGCAAGTGTGAGATCGCCCGGTTCTACAAGCTACAC
                    ************************************************************

HsCD00422619        GAAAGAAAGTGTGAACCTATCATCATGACTGTGCCCCGCAAGTCAGACCTCTTCCAGGAC
NM_032854.3         GAAAGAAAGTGTGAACCTATCATCATGACTGTGCCCCGCAAGTCAGACCTCTTCCAGGAC
XM_005258051.1      GAAAGAAAGTGTGAACCTATCATCATGACTGTGCCCCGCAAGTCAGACCTCTTCCAGGAC
XM_005258047.1      GAAAGAAAGTGTGAACCTATCATCATGACTGTGCCCCGCAAGTCAGACCTCTTCCAGGAC
XM_005258049.1      GAAAGAAAGTGTGAACCTATCATCATGACTGTGCCCCGCAAGTCAGACCTCTTCCAGGAC
                    ************************************************************

HsCD00422619        GATCTGTACCCGGATACGCCAGGCCCGGAGCCGGCCCTAGAAGCGGACGAATGGCTATCC
NM_032854.3         GATCTGTACCCGGATACGCCAGGCCCGGAGCCGGCCCTAGAAGCGGACGAATGGCTATCC
XM_005258051.1      GATCTGTACCCGGATACGCCAGGCCCGGAGCCGGCCCTAGAAGCGGACGAATGGCTATCC
XM_005258047.1      GATCTGTACCCGGATACGCCAGGCCCGGAGCCGGCCCTAGAAGCGGACGAATGGCTATCC
XM_005258049.1      GATCTGTACCCGGATACGCCAGGCCCGGAGCCGGCCCTAGAAGCGGACGAATGGCTATCC
                    ************************************************************

HsCD00422619        GGCCAGGACGCCGAACCCGTGCTCATTTCGCTGAGGGACGGCTATGTGCCCCCCAAGCAC
NM_032854.3         GGCCAGGACGCCGAACCCGTGCTCATTTCGCTGAGGGACGGCTATGTGCCCCCCAAGCAC
XM_005258051.1      GGCCAGGACGCCGAACCCGTGCTCATTTCGCTGAGGGACGGCTATGTGCCCCCCAAGCAC
XM_005258047.1      GGCCAGGACGCCGAACCCGTGCTCATTTCGCTGAGGGACGGCTATGTGCCCCCCAAGCAC
XM_005258049.1      GGCCAGGACGCCGAACCCGTGCTCATTTCGCTGAGGGACGGCTATGTGCCCCCCAAGCAC
                    ************************************************************

HsCD00422619        CGCGAGCTCCGGGTCACGAAGCGCAACATCCTGGACGTGCGCCCGCCCTCCGGCCCCCGC
NM_032854.3         CGCGAGCTCCGGGTCACGAAGCGCAACATCCTGGACGTGCGCCCGCCCTCCGGCCCCCGC
XM_005258051.1      CGCGAGCTCCGGGTCACGAAGCGCAACATCCTGGACGTGCGCCCGCCCTCCGGCCCCCGC
XM_005258047.1      CGCGAGCTCCGGGTCACGAAGCGCAACATCCTGGACGTGCGCCCGCCCTCCGGCCCCCGC
XM_005258049.1      CGCGAGCTCCGGGTCACGAAGCGCAACATCCTGGACGTGCGCCCGCCCTCCGGCCCCCGC
                    ************************************************************

HsCD00422619        CGCAGCCAGTCGGCCAGCGACGCCCCCTTG---TCGCAGCACACCCTGGAGACGCTGCTG
NM_032854.3         CGCAGCCAGTCGGCCAGCGACGCCCCCTTGTCGCAGCAGCACACCCTGGAGACGCTGCTG
XM_005258051.1      CGCAGCCAGTCGGCCAGCGACGCCCCCTTGTCGCAGCAGCACACCCTGGAGACGCTGCTG
XM_005258047.1      CGCAGCCAGTCGGCCAGCGACGCCCCCTTGTCGCAGCAGCACACCCTGGAGACGCTGCTG
XM_005258049.1      CGCAGCCAGTCGGCCAGCGACGCCCCCTTG---TCGCAGCACACCCTGGAGACGCTGCTG
                    ******************************    .*************************

HsCD00422619        GAAGAGATCAAGGCCCTCCGCGAGCGGGTGCAGGCCCAGGAGCAGCGCATCACGGCTCTG
NM_032854.3         GAAGAGATCAAGGCCCTCCGCGAGCGGGTGCAGGCCCAGGAGCAGCGCATCACGGCTCTG
XM_005258051.1      GAAGAGATCAAGGCCCTCCGCGAGCGGGTGCAGGCCCAGGAGCAGCGCATCACGGCTCTG
XM_005258047.1      GAAGAGATCAAGGCCCTCCGCGAGCGGGTGCAGGCCCAGGAGCAGCGCATCACGGCTCTG
XM_005258049.1      GAAGAGATCAAGGCCCTCCGCGAGCGGGTGCAGGCCCAGGAGCAGCGCATCACGGCTCTG
                    ************************************************************

HsCD00422619        GAGAACATGCTGTGCGAGCTGGTGGACGGCACGGACTAGTAGGACCCAGCTTTCTTGTAC
NM_032854.3         GAGAACATGCTGTGCGAGCTGGTGGACGGCACGGACTAG---------------------
XM_005258051.1      GAGAACATGCTGTGCGAGCTGGTGGACGGCACGGACTAG---------------------
XM_005258047.1      GAGAACATGCTGTGCGAGCTGGTGGACGGCACGGACTAG---------------------
XM_005258049.1      GAGAACATGCTGTGCGAGCTGGTGGACGGCACGGACTAG---------------------
                    ***************************************                     

HsCD00422619        AAAG
NM_032854.3         ----
XM_005258051.1      ----
XM_005258047.1      ----
XM_005258049.1      ----