Gene:
Gene Symbol:
lipA
Gene Name:
lipoyl synthase
Original Clone ID:
APC198.1_SG173_pMCSG19
Keyword:
partial cds
Species:
Bacillus subtilis subsp. subtilis str. 168
Type:
cDNA
Vector Name:
pMCSG19
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
BsCD00422656
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 843nts
Open reading frame: 51 to 893
ATTTTGGCGGGGATCTGGGTACCGAGAACCTGTACTTTCAATCCAATGTCAATACAAAGG
AGAACTATGCAGGGCTCAAAAAACTCATGCGGGAAAACAACCTGCATACCGTATGCGAAG
AAGCAAAGTGTCCGAATATTCATGAATGCTGGGCGGTCAGACGTACAGCGACATTTATGA
TTTTAGGTTCTGTTTGTACAAGAGCATGCCGTTTCTGTGCTGTCAAAACAGGCCTTCCAA
CTGAGCTTGATCTGCAAGAGCCGGAACGCGTAGCGGATTCAGTCGCGCTTATGAATTTGA
AACACGCGGTCATTACGGCTGTTGCGCGTGACGATCAGAAAGATGGCGGGGCAGGCATTT
TCGCGGAAACTGTCCGTGCGATCCGCAGAAAAAGCCCGTTCACAACAATTGAGGTGCTGC
CGTCTGATATGGGCGGAAACTACGATAACCTAAAAACCTTGATGGATACACGCCCAGACA
TCCTGAATCACAATATCGAAACGGTTCGCCGCCTGACGCCAAGAGTCCGCGCGCGTGCGA
CGTACGACCGTTCATTGGAATTTTTGCGCCGTGCGAAGGAAATGCAGCCTGACATTCCGA
CAAAATCAAGTATCATGATCGGACTCGGCGAAACAAAAGAAGAGATTATTGAAGTAATGG
ACGACCTCTTGGCGAACAACGTGGATATTATGGCAATCGGCCAATATCTGCAGCCGACGA
AGAAACACTTGAAAGTCCAAAAATACTACCATCCTGATGAATTTGCAGAATTAAAAGAAA
TCGCGATGCAAAAAGGCTTCAGCCATTGTGAAGCCGGTCCGCTTGTCCGTTCCTCTTACC
ATGCGGATGAACAAGTAAACGAAGCATCTAAAAAGCGTCAAGCACAAGCATAACATTGGA
AGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG
Protein sequence predicted based on nucleotide sequence
NTKENYAGLKKLMRENNLHTVCEEAKCPNIHECWAVRRTATFMILGSVCTRACRFCAVKT GLPTELDLQEPERVADSVALMNLKHAVITAVARDDQKDGGAGIFAETVRAIRRKSPFTTI EVLPSDMGGNYDNLKTLMDTRPDILNHNIETVRRLTPRVRARATYDRSLEFLRRAKEMQP DIPTKSSIMIGLGETKEEIIEVMDDLLANNVDIMAIGQYLQPTKKHLKVQKYYHPDEFAE LKEIAMQKGFSHCEAGPLVRSSYHADEQVNEASKKRQAQA*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t9g,N3K;a12g,E4E;a19g,T7A
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC198.1
Recommended expression in:
Not Applicable
Vector Name:
pMCSG19
Synonyms:
pMCSG-19
Sequencing Primer:
Forward:
ANForward
Reverse:
T7 terminator
Description:
Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
Comments:
pMCSG7 derivative: in vivo TVMV cleavage of MBP
Size (bp):
6441
Parent Vector:
None
Properties:
bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
204
235
bacterial origin
ori
ColE1-type bacterial origin of replication
4416
5098
promoter
T7
T7 promoter
1515
1531
protease cleavage site
TEV
TEV cleavage site cds
223
243
protease cleavage site
TVMV
TVMV protease cleavage site
286
306
repressor binding site
lac operator
LacO fragment
1490
1512
repressor protein gene
LacI
LacI coding sequence
2045
3004
selectable marker
AmpR
ampicillin resistance gene
5193
6053
tag
His
6xHis tag
268
285
tag
His 2
C-terminal 6xHis tag
140
157
tag
MBP
N-terminal MBP
337
1443
trxn termination sequence
T7 term
T7 terminator sequence
26
72
