VF_0087 (A. fischeri ES114 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  VF_0087
Gene Name:  sensory transduction protein kinase
Original Clone ID:
APC38667.1_SG253_pMCSG19
Keyword:
partial cds
Species:
Aliivibrio fischeri ES114
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Brian Feldmann
PSI
Midwest Center for Structural Genomics
VfCD00422783
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 705nts
Open reading frame: 51 to 755

CTTCTGGCGTGGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAATCAACTGG
ATGGGCTTACTTGTGTCAACCGAGCGATTTCTCAAAAGGGAGTATTGGTTTATGGGCAAC
CTATTTATAAAGCGCATACTCATGAGCTAGCATCAACAGAATGTCTAATGCGAATTCAAG
AGGACGACCAAATATTTGCTCCGGGACAGTTTTTACCAGCTGTTGAGGGGACGCATCTCT
ATACTCGCTTAAGTAGAGAAATGATTTCGAGTATCTTTGAGATCATGCAGTCGAATGATT
TGACGTTCTCGATTAATTTATCTCCTCAAGATTTATTAAGTGATAGAACACTAGATTTAT
TAGAGTCATCCATGATCCGTTTAGATAACCCAATGCGAGTAGGATTTGAAGTGTTAGAAA
CGGAACAAATTCAAGATTATGGGCATATCATTGAGGTGTGTAATCATTTCCGACGCTTAG
GTGCGCGAATTATTATTGATGATTTCGGTTCAGGTTATTCCAATATTGATGCGATCATGA
AATTAGAGCCTCAGGTGATTAAGCTTGATGGTTCAATCATCAAAAATGTACATCAAGATA
AGCGACAGCGTTTGATCTCTCAGCAAATGGTACAGCTTTGTCATGTATTAGATGCCAAAG
CTGTGGCCGAGTTTGTTCATAACCAAGAGGTGTGTTCAATTGTTGAAGATATGGGTGTGG
ATTACTTACAAGGTTACTTTTTAGGTGAGCCTTAACATTGGAAGTGGATAACGGATCCGA
ATTCGAGCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

NQLDGLTCVNRAISQKGVLVYGQPIYKAHTHELASTECLMRIQEDDQIFAPGQFLPAVEG
THLYTRLSREMISSIFEIMQSNDLTFSINLSPQDLLSDRTLDLLESSMIRLDNPMRVGFE
VLETEQIQDYGHIIEVCNHFRRLGARIIIDDFGSGYSNIDAIMKLEPQVIKLDGSIIKNV
HQDKRQRLISQQMVQLCHVLDAKAVAEFVHNQEVCSIVEDMGVDYLQGYFLGEP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t13g,W5G;t30c,N10N;
g171t,A57A;t189c,L63L;
c205a,R69R;g351a,V117V;
t420c,F140F;a435g,A145A;
c493t,L165L;c522t,G174G;
t528c,I176I;c666t,G222G
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Tested_Not_Found
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC38667.1
Recommended expression in:
Not Applicable