Gene:
Gene Symbol:
VF_1603
Gene Name:
diguanylate cyclase
Original Clone ID:
APC38682_SG253_pMCSG19
Keyword:
full length
Species:
Aliivibrio fischeri ES114
Type:
cDNA
Vector Name:
pMCSG19
Format:
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
High number of silent and/or missense mutations in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Brian Feldmann
PSI
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
VfCD00422789
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1200nts
Open reading frame: 1 to 1200
ATGTACACATATGTTGCTCGTCAACCAATCTTAGACCTAAGTAAAAATACGATTGGTTAC
GAATTATTATTTCGAGACGGTCCAAAAAACACTTTTCCACTTGTTGAAGCAGAAGAAGCA
ACGAGCCGACTTTTATCCGATCATTTTTTAAGCTCATACTGCAATGCAACAAATGATAAA
TTAGGCTTTGTTAATTTCCCTCAACAAAGCTTATTAAATCTAGTTCCTACGCTTTTTAAT
CCAGATAACTTAGTAGTTGAAATACTAGAAGACTGCATTCCAAGCCCTGAATTATTAGTA
GCAATTAAACGTCTTGCCAAGCTAGGTTACAAACTTGCTCTTGATGATTTTGAGCCAAAT
CCAGATTGGACTCCTTTTCTACCTTACATTGATATCATCAAGTTTGATATGCGTACAATG
CCAATTCTAAAGGCAAAGCTATTCATTCGTCATTGTGAGAAATACAATATTAAGTTTTTA
GCTGAGAAAGTAGAAACTTACGAAGAATTTGAGCAGGCAAAAGAAGCTGGCTTTACTTAT
TTCCAAGGCTATTTTTTCAGTAAACCTGAAATGCTTCAGCAACGAGCAATTCAACCAAAT
CAACTTGCGATTATTCAGTTATACCAAGAGATCTCTGCACCTAGTGTCGATTTCAATACC
GTAGAAAAATACATCACTCAAGATGTTTCACTTTCTTATAAACTGCTACGATTTGTTAAC
TCATCTTCTATTATTGACAAGCCAATCACCTCTTTTAAGCAAGCATTAGTCTATTTGGGT
GAAACTAAGCTTCGTCAATTTGTATCATTGGTAGCAGTTGCTCATGCAACTCAACACAAA
CCACAATCTCTATACACGTTATCAATCCACCGTGCTAGATTGTGTGAGTTAATCGTAACT
AAAACGGATATTGAATTAGAACCAAGCCAAGCTTTTTTAGTTGGACTGTTTTCTTTACTT
GAGCCTTTACTAGATCAACCGCTTGAAACACTGATTCAGCATTTGCCTATTTCTATTGAA
ATAAAACAAGCGCTCACTGAACGTAAAGGATTATTAGGAAAGCTGATATTAGCTATCGAG
AGCTATGAAAAAGCAGATTGGAGCGTCGTAGCCGAATGTTGTCGATCTCTAAATATTACA
GAAAAAGAATTTACCGCTTGTTATAAAGAATCTGCCAAATGGGTAGATGAGATAGCAAAA
Protein sequence predicted based on nucleotide sequence
MYTYVARQPILDLSKNTIGYELLFRDGPKNTFPLVEAEEATSRLLSDHFLSSYCNATNDK LGFVNFPQQSLLNLVPTLFNPDNLVVEILEDCIPSPELLVAIKRLAKLGYKLALDDFEPN PDWTPFLPYIDIIKFDMRTMPILKAKLFIRHCEKYNIKFLAEKVETYEEFEQAKEAGFTY FQGYFFSKPEMLQQRAIQPNQLAIIQLYQEISAPSVDFNTVEKYITQDVSLSYKLLRFVN SSSIIDKPITSFKQALVYLGETKLRQFVSLVAVAHATQHKPQSLYTLSIHRARLCELIVT KTDIELEPSQAFLVGLFSLLEPLLDQPLETLIQHLPISIEIKQALTERKGLLGKLILAIE SYEKADWSVVAECCRSLNITEKEFTACYKESAKWVDEIAK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c285t,S95S;t360c,N120N;
t372c,T124T;t384g,P128P;
g432a,K144K;t528a,A176A;
a708g,L236L;c720t,N240N;
g810a,L270L;a813c,V271V;
a816t,A272A;a843g,P281P;
g888a,E296E;c991t,L331L;
g1005a,L335L;a1041c,E347D;
t1074c,A358A;g1103a,S368N;
a1110t,V370V;a1125c,R375R
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC38682
Recommended expression in:
Not Applicable
Vector Name:
pMCSG19
Synonyms:
pMCSG-19
Sequencing Primer:
Forward:
ANForward
Reverse:
T7 terminator
Description:
Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV, His, TEV, NA; amp resistance; ligation independent cloning (LIC).
Comments:
pMCSG7 derivative: in vivo TVMV cleavage of MBP
Size (bp):
6441
Parent Vector:
None
Properties:
bacterial expression, improves solubility of insert, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
Ligation independent cloning (LIC) site
204
235
bacterial origin
ori
ColE1-type bacterial origin of replication
4416
5098
promoter
T7
T7 promoter
1515
1531
protease cleavage site
TEV
TEV cleavage site cds
223
243
protease cleavage site
TVMV
TVMV protease cleavage site
286
306
repressor binding site
lac operator
LacO fragment
1490
1512
repressor protein gene
LacI
LacI coding sequence
2045
3004
selectable marker
AmpR
ampicillin resistance gene
5193
6053
tag
His
6xHis tag
268
285
tag
His 2
C-terminal 6xHis tag
140
157
tag
MBP
N-terminal MBP
337
1443
trxn termination sequence
T7 term
T7 terminator sequence
26
72
