YE4063 (Y. enterocolitica subsp. enterocolitica 8081 )
In pMCSG19B (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  YE4063
Gene Name:  EAL domain-containing protein
Original Clone ID:
APC38721.2_SG255_pMCSG19B
Keyword:
partial cds
Species:
Yersinia enterocolitica subsp. enterocolitica 8081
Type:
cDNA
Vector Name:
pMCSG19B
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
High number of silent and/or missense mutations in CDS.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Brian Feldmann
PSI
Midwest Center for Structural Genomics
YeCD00422795
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 681nts
Open reading frame: 49 to 729

TCTGGCGTGGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGATCGTAATAAA
GGACAGAGCTTCTGGCGGCAATGTCAGCGTCGGTACACTTTTCAACCCATCTACCGCACC
TCTGGGGCGCTGCTGGCTATTGAGCTGCTTACGGCGGTTTTCCATCCTTCCTCGCCGGAT
ACCCGCTTGAATCCGGAAGATTACTTCAGTGCTATTAGTATCCGCGCCCGTCTGGATGTA
GTGCTGGAGCAGTTGAAGATGGTCAAGCAATGGCATGCTATATTTGTGCATCACTCGGTG
TTGGTCTCGATTAACATTGATGGTCAGGCTTTGATGGCGATGCAGGACGACTTGCAGGCT
AAGGTGTTAATTGATGCCATGCCTTACATTCGCTTTGAATTGCTCGAGCATGTCGATACC
TCTCTTGATACCCCCTTTGCACGTATTGCCGAGGCAGACCGGCTATGGCTGGATGATTTT
GGCAGCGGGCTGGCGAATTTCACTTCATTTATTAGTTGGCGCTATGAATACATCAAAGTC
GCCAGAGACTTGTTTATCGTGCTGCAAGAAAGTGATGTTGGGAACCAACTGTTTTTCAAA
CTGGTGACCCTGATGGGAAGTTATAGCAAAGGCGTGATTGTTGAAGGGGTGGAAACCTCG
CAAGAATGGGCGATGGTACAGCAGTCTGATGCGGTTGCTGCACAAGGCTATTATCTCTCC
CGACCTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

DRNKGQSFWRQCQRRYTFQPIYRTSGALLAIELLTAVFHPSSPDTRLNPEDYFSAISIRA
RLDVVLEQLKMVKQWHAIFVHHSVLVSINIDGQALMAMQDDLQAKVLIDAMPYIRFELLE
HVDTSLDTPFARIAEADRLWLDDFGSGLANFTSFISWRYEYIKVARDLFIVLQESDVGNQ
LFFKLVTLMGSYSKGVIVEGVETSQEWAMVQQSDAVAAQGYYLSRP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t60c,P20P;t66c,Y22Y;
t69c,R23R;c75t,S25S;
t78g,G26G;c93t,I31I;
a108g,A36A;t114c,F38F;
c117t,H39H;c120t,P40P;
a123c,S41S;a126g,S42S;
t135c,T45T;t138c,R46R;
c139t,L47L;a165t,A55A;
c168t,I56I;a175c,S59R;
t184c,L62L;a187g,N63D;
g192a,V64V;t210g,N70K;
t216c,V72V;a219g,K73K;
g231t,A77A;t402c,A134A;
a405g,E135E;c414g,R138R;
t415c,L139L;c468t,S156S;
g480a,E160E;t501c,D167D;
c531t,V177V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC38721.2
Recommended expression in:
Not Applicable