VF_A0681 (A. fischeri ES114 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  VF_A0681
Gene Name:  hypothetical protein
Original Clone ID:
APC36761.2_SG212_pMCSG19
Keyword:
partial cds
Species:
Aliivibrio fischeri ES114
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
High number of silent and/or missense mutations in CDS.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Jessica Bearden
PSI
Midwest Center for Structural Genomics
VfCD00422812
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 639nts
Open reading frame: 51 to 689

CTTCTGGCGTGGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCCGTCCTGTCT
GGTATGGCGAGCCTGTGGATATTCAGCCAGAACACAGAGACATGATGTTGGTGGTGGATT
TATCCGGATCTATGGCAGAAGAAGACATGAAAACCAGTAATGGTGATTTTGTTGACCGCT
TAACCGCTGTTAAGCAAGTGGTGTCCGATTTTATTGACCAACGTAAAGGCGATCGCCTTG
GCTTGGTGCTGTTTGGTGATCACGCTTATCTTCAAACGCCTCTCACCTTTGATAGAAACA
CAGTACGTGAACAATTAGATCGTACTGTGCTAAATCTTGTAGGCCAACGAACTGCAATCG
GTGAAGGTTTAGGACTGGCAACCAAAACCTTTATTGAAAGTAATGCACCACAAAGAACCA
TTATTTTATTAAGTGATGGTGCTAATACGGCAGGTGTACTAGAGCCATTAGAAGCGGCAC
AACTGGCAAAAGATAATCATGCCAAGATTTACACCGTTGGTATTGGCGCTGGTGAAATGC
AAGTACGTGGATTCTTTGGAAAACAAACGGTAAATACTGCTCGTGATCTGGATGAAGATA
CCTTAACCAAGATTGCGACAATGACTGGCGGACAATATTTCCGTGCTCGTAACGCCGATG
AACTTGCAGAAATTTATCAAACCATTTAACATTGGAAGTGGATAACGGATCCGAATTCGA
GCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

RPVWYGEPVDIQPEHRDMMLVVDLSGSMAEEDMKTSNGDFVDRLTAVKQVVSDFIDQRKG
DRLGLVLFGDHAYLQTPLTFDRNTVREQLDRTVLNLVGQRTAIGEGLGLATKTFIESNAP
QRTIILLSDGANTAGVLEPLEAAQLAKDNHAKIYTVGIGAGEMQVRGFFGKQTVNTARDL
DEDTLTKIATMTGGQYFRARNADELAEIYQTI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c78a,G26G;a189t,L63L;
g261a,E87E;g267a,L89L;
c276t,T92T;c300a,R100R;
c303t,T101T;g306a,A102A;
t309c,I103I;c384t,S128S;
g390t,G130G;a426g,A142A;
g429a,A143A;a435g,L145L;
c528t,T176T;a594t,R198R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC36761.2
Recommended expression in:
Not Applicable