Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
APC37678.2_SG230_pMCSG19
Keyword:
partial cds
Species:
Bacillus subtilis subsp. subtilis str. 168
Type:
cDNA
Vector Name:
pMCSG19
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
High number of silent and/or missense mutations in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Kimberly Buck
PSI
Midwest Center for Structural Genomics
BsCD00422823
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1185nts
Open reading frame: 1 to 1185

AAAGTCGAGAAAGTCTCTAAGATTTTTGGGAAACAAACAAAGAAGGCAGTTCAAATGCTT
GCCAACGGAAAGGCAAAAAAAGAGATCCTGAAAGCAACCGGATCAACCGTTGGGGTTAAT
CAGGCAGATTTTGAAGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGC
GGTAAATCAACTTTAGTACGGATGTTAAACCGGCTTATTGAACCGACTGCCGGAAATATT
TACATTGATGGTGACATGATAACAAATATGTCAAAAGACCAGCTCCGGGAAGTCCGCCGG
AAAAAAATCAGCATGGTCTTCCAAAAGTTCGCTCTGTTTCCGCACAGAACGATACTTGAA
AACACAGAATACGGACTGGAGCTTCAAGGTGTAGACAAACAAGAGCGGCAGCAAAAAGCG
CTTGAGTCTCTTAAGCTCGTTGGACTGGAAGGATTTGAACACCAATATCCCGACCAGCTT
TCCGGCGGGATGCAGCAGCGTGTCGGCCTTGCCCGCGCGTTGACGAATGACCCGGACATC
CTTCTGATGGACGAAGCGTTCAGTGCGCTCGATCCACTGATTCGTAAAGATATGCAGGAT
GAGCTTCTTGATCTTCACGATAATGTCGGAAAAACAATTATCTTTATTACGCATGACTTG
GACGAAGCGTTGCGGATCGGCGACAGAATTGTCCTAATGAAGGACGGCAACATCGTTCAA
ATCGGCACGCCTGAAGAAATCCTGATGAATCCATCTAACGAATATGTTGAGAAATTCGTC
GAAGATGTTGATTTGTCTAAAGTCCTCACAGCAGGCCACATTATGAAACGCGCTGAAACA
GTGCGGATTGATAAAGGGCCTCGCGTAGCATTGACACTGATGAAAAACCTTGGAATTTCT
TCAATTTATGCAGTGGATAAACAAAAGAAATTATTAGGTGTTATCTATGCGTCTGATGCG
AAAAAAGCGGCTGAGTCTGATTTGTCACTTCAAGACATCTTGAATACAGAATTTACGACT
GTACCAGAGAATACGTATCTGACTGAGATTTTTGACGTTGTGTCCGATGCGAATATTCCA
ATTGCGGTTGTGGATGAGAAGCAAAGAATGAAAGGAATTGTCGTAAGAGGCGCGCTGATT
GGCGCACTTGCCGGCAATAACGAGTATATCAATGCTGAAGGCACT

Protein sequence predicted based on nucleotide sequence

KVEKVSKIFGKQTKKAVQMLANGKAKKEILKATGSTVGVNQADFEVYDGEIFVIMGLSGS
GKSTLVRMLNRLIEPTAGNIYIDGDMITNMSKDQLREVRRKKISMVFQKFALFPHRTILE
NTEYGLELQGVDKQERQQKALESLKLVGLEGFEHQYPDQLSGGMQQRVGLARALTNDPDI
LLMDEAFSALDPLIRKDMQDELLDLHDNVGKTIIFITHDLDEALRIGDRIVLMKDGNIVQ
IGTPEEILMNPSNEYVEKFVEDVDLSKVLTAGHIMKRAETVRIDKGPRVALTLMKNLGIS
SIYAVDKQKKLLGVIYASDAKKAAESDLSLQDILNTEFTTVPENTYLTEIFDVVSDANIP
IAVVDEKQRMKGIVVRGALIGALAGNNEYINAEGT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a189c,S63S;g196a,V66I;
g200c,R67P;t205g,L69V;
g225c,P75P;c230g,A77G;
g232c,G78R;a256c,M86L;
a264c,T88T;a273c,S91S;
c279a,D93E;g287c,R96P;
g296c,R99P;g311a,S104N;
a323c,Q108P;t337g,F113V;
a364c,a366c,T122P;g381a,E127E;
a386c,Q129P;t390g,G130G;
t392g,V131G;g394a,D132N;
a397c,K133Q;g403a,g405a,E135K;
g426a,E142E;g439c,V147L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in APC37678.2
Recommended expression in:
Not Applicable