MM_2581 (M. mazei Go1 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MM_2581
Gene Name:  pyruvate formate-lyase activating protein-like protein
Original Clone ID:
APC63383.2_SG191_pMCSG19
Keyword:
partial cds
Species:
Methanosarcina mazei Go1
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
Low quality silent and/or missense mutations found in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI
Midwest Center for Structural Genomics
MmCD00422857
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 747nts
Open reading frame: 35 to 781

GGGTACCGAGAACCTGTACTTCCAATCCAATGCCAACGGAGACCTTTACTCTCTTATNTA
CGGTACTGTTTCAAGCGAAGCTATAGACCCTGTGGAAAAGAAGCCGCTTTACCATTTCTT
TCCGGGTTCCTATGTTTATTCGGTAGGTACTATAGGGTGCAATTTCCGCTGTAAGCACTG
CCAGAACTGGTCAATCTCTCAGGTCTGCCTGGAAGACGCATACACAAAAGACATTCTTCC
GGAAGAGCTTGTGGAAAGAGCCCTTTTATCAGACTCGAAGTCCATTGCCTGGACATATAA
CGAGCCTACTATCTGGCACGAGTATACCTATGAGAGTGCAAAACTGGCAAAAGAAGCGGG
TCTGGGTACTATTTACGTAACAAACGGTTATATGACTCCCGAATCGCTAAAACATATCGC
TCCTTATCTGGATGCAGCCAACATCGATATTAAAGCTTTCAACGAAAAGTTCTATCACGA
TATTGCCAGTGCAAAACTGGCTCCCGTGCTTGAAGCTTCAGCTCTTGCAAAGGAGCTGGG
GATCCATGTGGAGATAACGACCCTGATTATTCCCGGAGTAAATGATTCTCTTGACGAGCT
AAGGGAACTTTCAAAATGGGTCTATAAAAACCTCGGTCCAGATACCCCCCTGCATTTTAC
GCGCTTCCATCCGCAGTACAAGATGCAGGACCTTTCCCCGACACCTGTAAAAACTATGGA
GAATGCCTGTAAAATCGCAACCGAAGAGGGGATGAAATATGTCTATATGGGCAATGTCTA
ACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

NGDLYSLYGTVSSEAIDPVEKKPLYHFFPGSYVYSVGTIGCNFRCKHCQNWSISQVCLED
AYTKDILPEELVERALLSDSKSIAWTYNEPTIWHEYTYESAKLAKEAGLGTIYVTNGYMT
PESLKHIAPYLDAANIDIKAFNEKFYHDIASAKLAPVLEASALAKELGIHVEITTLIIPG
VNDSLDELRELSKWVYKNLGPDTPLHFTRFHPQYKMQDLSPTPVKTMENACKIATEEGMK
YVYMGNV*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t24n
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC63383.2
Recommended expression in:
Not Applicable