TLE1 (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  TLE1
Gene Name:  transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)
Original Clone ID:
FLH195838.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
Plate source, forward and reverse reads, produced low quality DNA; tube source, forward and reverse reads contig'd to each other but did not contig to refseq; BLAST search returned HsCD00040841
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00042290
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UNAVAILABLE
No restriction
Special MTA: None
Insert sequence: 2310nts
Open reading frame: 1 to 2310

ATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCTGCAGGCCAGCCCTTCAAGTTC
ACTATCCCGGAGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTAT
CACAGCCTTAAATTGGAATGTGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCAC
TATGTGATGTATTATGAAATGTCATATGGATTAAACATTGAAATGCACAAACAGACTGAA
ATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATCCCATTTCTGTCTCAGGAACAT
CAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAGGTGACCATGGCAGAGTTGAAT
GCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTTTCTCATGGCCACGGACCCCCA
GTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCTGGAATCCCGCCCCTCGGGGGC
AGTGCCGGCCTTCTTGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAA
GATGACAAGAAGCACCACGATGCAGAGCACCACAGAGACAGAGAGCCGGGCACAAGTAAT
TCCCTCCTGGTCCCAGACAGTCTAAGAGGCACAGATAAACGCAGAAATGGACCTGAATTT
TCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGACTCCAGCCACTATGACAGTGAT
GGTGACAAAAGCGATGACAACTTAGTTGTGGATGTGTCTAATGAGGACCCTTCTTCTCCG
CGAGCAAGCCCTGCCCACTCGCCCCGGGAAAATGGAATCGACAAAAATCGCCTGCTAAAG
AAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCGGCAAGTTCCACTTCTTTGAAA
TCCAAAGAAATGAGCTTGCATGAAAAAGCCAGCACGCCTGTTCTGAAATCCAGCACACCA
ACGCCTCGGAGCGACATGCCAACGCCGGGCACCAGCGCCACTCCAGGCCTCCGTCCAGGT
CTCGGCAAGCCTCCAGCCATAGACCCCCTCGTTAACCAAGCGGCAGCTGGCTTGAGGACA
CCCCTGGCAGTGCCCGGCCCATATCCTGCTCCTTTTGGGATGGTCCCCCACGCTGGCATG
AACGGCGAGCTGACCAGCCCAGGCGCTGCCTACGCCAGTTTACACAACATGTCGCCCCAG
ATGAGCGCCGCAGCCGCCGCGGCCGCCGTGGTGGCCTACGGGCGCTCCCCCATGGTGGGG
TTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCTCCAAACCTGGCAGGAATCCCT
GGGGGGAAACCTGCATACTCCTTCCACGTTACTGCAGACGGTCAGATGCAGCCTGTCCCT
TTTCCCCCCGACGCCCTCATCGGACCCGGAATCCCCCGGCATGCTCGCCAGATCAACACC
CTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATCAGCAACCCCACGAGACACGTGTAC
ACAGGCGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGCCACCCTGGCAATAAGAGCCCT
GTCTCCCAGCTCGACTGTCTGAACAGAGACAATTATATCCGTTCCTGTAAATTGCTACCC
GATGGCTGCACTCTCATAGTGGGAGGGGAAGCCAGTACTTTGTCCATTTGGGACCTGGCG
GCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCGGCCCCCGCCTGCTACGCCCTG
GCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTG
TGGGATCTGCACAACCAGACACTAGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGC
TGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACGGGTGGTTTGGACAACACAGTC
AGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACCTCCCAGATC
TTCTCCCTGGGGTACTGCCCCACCGGGGAGTGGCTGGCAGTGGGCATGGAGAGCAGCAAT
GTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTACCAGCTGCACCTGCATGAGAGCTGC
GTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTTGTGAGTACTGGAAAAGATAAC
CTCCTCAATGCTTGGCGGACCCCCTATGGAGCCAGCATATTCCAGTCCAAAGAGTCCTCG
TCAGTGCTTAGCTGTGACATCTCTGTGGATGATAAGTACATAGTCACTGGCTCGGGGGAC
AAGAAGGCTACAGTCTATGAAGTCATCTAC

Protein sequence predicted based on nucleotide sequence

MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRH
YVMYYEMSYGLNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELN
AIIGQQQLQAQHLSHGHGPPVPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIK
DDKKHHDAEHHRDREPGTSNSLLVPDSLRGTDKRRNGPEFSNDIKKRKVDDKDSSHYDSD
GDKSDDNLVVDVSNEDPSSPRASPAHSPRENGIDKNRLLKKDASSSPASTASSASSTSLK
SKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAIDPLVNQAAAGLRT
PLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAVVAYGRSPMVG
FDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINT
LNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLP
DGCTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAV
WDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQI
FSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDN
LLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2763
Start on reference sequence 451
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable