NASP (Homo sapiens) in pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  NASP
Gene Name:  nuclear autoantigenic sperm protein (histone-binding)
Original Clone ID:
FLH195854.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00042292
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2364nts
Open reading frame: 1 to 2364

ATGGCCATGGAGTCCACAGCCACTGCCGCCGTCGCCGCGGAGCTGGTTTCTGCCGACAAA
ATTGAAGATGTTCCTGCTCCTTCTACATCTGCAGATAAAGTGGAGAGTCTGGATGTGGAT
AGTGAAGCTAAGAAACTATTGGGTTTAGGACAGAAACATCTGGTGATGGGGGATATTCCA
GCAGCTGTCAATGCATTCCAGGAAGCAGCTAGTCTTTTAGGTAAGAAGTATGGAGAGACA
GCTAATGAGTGTGGAGAAGCCTTCTTTTTCTATGGGAAATCACTTCTGGAGTTGGCAAGA
ATGGAGAATGGTGTGTTGGGAAACGCCTTGGAAGGTGTGCATGTGGAAGAGGAAGAAGGA
GAAAAAACAGAAGATGAATCTCTGGTAGAAAATAATGATAACATAGATGAGGAAGCAAGG
GAAGAGTTGAGAGAACAGGTTTATGACGCCATGGGAGAAAAAGAAGAAGCCAAAAAAACA
GAAGACAAGTCTTTGGCAAAGCCTGAAACTGATAAAGAACAGGACAGTGAAATGGAGAAG
GGTGGAAGAGAAGATATGGATATAAGTAAATCTGCAGAGGAGCCACAGGAAAAAGTTGAC
TTGACTCTAGATTGGTTAACTGAAACCTCTGAAGAGGCAAAAGGAGGAGCAGCACCAGAA
GGACCGAATGAAGCTGAGGTCACTTCTGGGAAGCCAGAACAGGAAGTACCAGATGCTGAG
GAAGAAAAATCAGTTTCTGGAACTGATGTCCAAGAAGAGTGCAGAGAAAAAGGAGGTCAG
GAGAAGCAGGGAGAGGTAATTGTGAGCATAGAGGAGAAGCCAAAAGAAGTTTCAGAAGAG
CAGCCTGTGGTGACTCTAGAAAAGCAGGGCACTGCAGTGGAGGTAGAAGCAGAGTCTTTA
GACCCGACAGTCAAGCCAGTGGATGTGGGTGGGGACGAGCCAGAGGAGAAGGTAGTTACC
TCTGAAAACGAGGCAGGAAAGGCGGTTCTTGAACAACTGGTAGGTCAAGAAGTACCACCT
GCTGAAGAGTCACCAGAGGTGACAACAGAGGCTGCAGAGGCCTCAGCTGTAGAGGCTGGA
TCAGAAGTCTCTGAAAAGCCTGGGCAGGAGGCTCCAGTTCTCCCTAAGGATGGTGCAGTC
AATGGACCGTCAGTTGTAGGAGATCAGACTCCTATTGAACCACAGACTTCTATAGAAAGA
CTGACAGAAACAAAAGATGGCTCAGGACTAGAGGAGAAGGTCAGGGCAAAGCTGGTTCCT
AGTCAGGAGGAGACTAAGCTGTCTGTAGAAGAGTCTGAGGCAGCTGGAGATGGGGTTGAT
ACCAAGGTAGCCCAGGGAGCTACTGAGAAATCACCTGAAGACAAAGTTCAGATAGCTGCT
AATGAAGAGACACAAGAGAGAGAAGAACAGATGAAAGAGGGTGAAGAAACTGAAGGCTCA
GAAGAGGATGATAAAGAAAATGATAAGACCGAAGAAATGCCAAATGATTCAGTCCTTGAA
AACAAGTCTCTTCAAGAAAATGAGGAGGAGGAGATTGGGAACCTAGAGCTTGCCTGGGAT
ATGCTGGATTTAGCAAAGATCATTTTTAAAAGGCAAGAAACAAAAGAAGCACAGCTTTAT
GCTGCCCAGGCACATCTTAAACTCGGAGAAGTTAGTGTTGAATCTGAAAACTATGTGCAA
GCTGTGGAGGAGTTCCAGTCCTGCCTTAACCTGCAGGAACAGTACCTGGAAGCCCACGAC
CGTCTCCTTGCAGAGACCCACTACCAGCTGGGCTTGGCTTATGGGTACAACTCTCAGTAT
GATGAGGCAGTGGCACAGTTCAGCAAATCTATTGAAGTCATTGAGAACAGAATGGCTGTA
CTAAACGAGCAGGTGAAGGAGGCTGAAGGATCGTCTGCTGAATACAAGAAAGAAATTGAG
GAACTAAAGGAACTGCTACCCGAAATTAGAGAGAAGATAGAAGATGCAAAGGAGTCTCAG
CGTAGTGGGAATGTAGCTGAACTGGCTCTGAAAGCTACTCTGGTGGAGAGTTCTACTTCA
GGTTTCACTCCTGGTGGAGGAGGCTCTTCAGTCTCCATGATTGCCAGTAGAAAGCCAACA
GACGGTGCTTCCTCATCAAATTGTGTGACTGATATTTCCCACCTTGTCAGAAAGAAGAGG
AAACCAGAGGAAGAGAGTCCCCGGAAAGATGATGCAAAGAAAGCCAAACAAGAGCCGGAG
GTGAACGGAGGCAGTGGGGATGCTGTCCCCAGTGGAAATGAAGTTTCGGAAAACATGGAG
GAGGAGGCTGAGAATCAGGCTGAAAGCCGGGCAGCAGTGGAGGGGACAGTGGAGGCTGGA
GCTACAGTTGAAAGCACTGCATGT

Protein sequence predicted based on nucleotide sequence

MAMESTATAAVAAELVSADKIEDVPAPSTSADKVESLDVDSEAKKLLGLGQKHLVMGDIP
AAVNAFQEAASLLGKKYGETANECGEAFFFYGKSLLELARMENGVLGNALEGVHVEEEEG
EKTEDESLVENNDNIDEEAREELREQVYDAMGEKEEAKKTEDKSLAKPETDKEQDSEMEK
GGREDMDISKSAEEPQEKVDLTLDWLTETSEEAKGGAAPEGPNEAEVTSGKPEQEVPDAE
EEKSVSGTDVQEECREKGGQEKQGEVIVSIEEKPKEVSEEQPVVTLEKQGTAVEVEAESL
DPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQLVGQEVPPAEESPEVTTEAAEASAVEAG
SEVSEKPGQEAPVLPKDGAVNGPSVVGDQTPIEPQTSIERLTETKDGSGLEEKVRAKLVP
SQEETKLSVEESEAAGDGVDTKVAQGATEKSPEDKVQIAANEETQEREEQMKEGEETEGS
EEDDKENDKTEEMPNDSVLENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQLY
AAQAHLKLGEVSVESENYVQAVEEFQSCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQY
DEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQ
RSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKR
KPEEESPRKDDAKKAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQAESRAAVEGTVEAG
ATVESTAC

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2463
Start on reference sequence 97
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable