rpsA (E. faecalis )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  rpsA
Gene Name:  30S ribosomal protein S1
Original Clone ID:
EfR127-14.3
Keyword:
targeted domain
Species:
Enterococcus faecalis
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
Low quality silent and/or missense mutations found in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
EfCD00422995
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1212nts
Open reading frame: 51 to 1262

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGACAGATT
TTGAAGACAAACAAGTAGCAAACAACGAAACAATGGAAGATGCAATGAACAGCGTTCAAG
AAGTCAGTGTTGGCGATATTGTTAAAGGCGAAGTTTTAGCCATTGAAGACAAACAAGTTG
TTGTCGGCATTGAAGGAGCGGGCGTTGAAGGCGTTGTTCCTGCAAAGGAATTATCGACTT
TACCAGTTGAAGATGTACACGAACTTGTGAAAGTCGGTGATGTGTTAGATTTAGTTGTTA
TCACATCAATTGGCAAAGATAAAGAAAACGGCAGTTATTTACTGTCAAAACGCCGTTTAG
ATGCTAAAAAAGTTTGGGAAGAAATTGAACAAGATTTCCAAGCGGGCAAAGTAATCGAAG
CACCTGTTACAAATGTTGTTAAAGGTGGTTTGGTTGTTGATGTCGGTGTTCGCGGATTTG
TTCCTGCCTCAATGGTTGAAGATCATTTTGTTGCAGATTTTTCTGAATATAAAGGTCAAA
CACTAGCATTTAAAATTATTGAAATCGAACCTTCAGAAAACCGCTTAATTCTTTCACATA
AAGCAGTTGTTGAAGCTGAAAAAGAATCGAAAAAAGAAGAATTACTTTCTTCTTTACATG
ACGGAGACGTTGTTGAAGGGAAAGTAGCTCGTTTAACAGATTTCGGCGCATTCATTGATT
TAGGTGGCGTGGATGGTTTAGTTCATGTTTCTGAAATCGCTCATCAGCATGTGGGCAAAC
CAAGTGATGCGTTAACTGTTGGCGAAGACGTTCAAGTAAAAATTCTTTCAATTAATCCAG
AAGAAGGACGTATTTCTTTATCTATTAAAGAAACATTGCCTGGACCATGGACAGATATCG
AAACAAAAGCAGCCGCTGGGACTGTTTTAGATGGCACAGTAAAACGCCTAACAAGCTTCG
GTGCCTTTGTGGAAGTTTTCCCTGGTGTGGAAGGATTAGTGCATATTTCTCAAATTTCTC
ACAAGCACATTGCAACGCCTCATGAAGTTCTTCATGAAGGGGATCAAGTACAAGTGAAAG
TTTTAGAAGTTAATCCAGAGGAACATCGTATTGCTTTAAGTATCAAAGCGTTAGAACCAA
AACCTGAGTCTCAAGAAGAACCAAAAGAAGAACAGGAATATGAATTGCCTGAAGAAAATA
CTGGCTTTACCATGGGCGATATTTTAGGTGACGCTTTATCTGATGCGAAAGAAGAGGAAT
AG

Protein sequence predicted based on nucleotide sequence

MTDFEDKQVANNETMEDAMNSVQEVSVGDIVKGEVLAIEDKQVVVGIEGAGVEGVVPAKE
LSTLPVEDVHELVKVGDVLDLVVITSIGKDKENGSYLLSKRRLDAKKVWEEIEQDFQAGK
VIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSEYKGQTLAFKIIEIEPSENRLI
LSHKAVVEAEKESKKEELLSSLHDGDVVEGKVARLTDFGAFIDLGGVDGLVHVSEIAHQH
VGKPSDALTVGEDVQVKILSINPEEGRISLSIKETLPGPWTDIETKAAAGTVLDGTVKRL
TSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLHEGDQVQVKVLEVNPEEHRIALSIKA
LEPKPESQEEPKEEQEYELPEENTGFTMGDILGDALSDAKEEE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in EfR127
Recommended expression in:
Not Applicable