lipA ([. ducreyi 35000HP )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  lipA
Gene Name:  lipoyl synthase
Original Clone ID:
APC62970.3_SG184_pMCSG19
Keyword:
partial cds
Species:
[Haemophilus] ducreyi 35000HP
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
HdCD00423220
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 801nts
Open reading frame: 1 to 801

ACTAATTCAGCGAAAATTGATAGCATTAAATATGGTATGCGTCGCCACGGGTTACATTCA
GTTTGTGAAGAAGCATCTTGTCCAAATTTACATGAATGTTTTAATCATGGTACTGCCACT
TTTATGATTATGGGCGCAATTTGTACTCGTCGTTGTCCGTTTTGTGATGTTGCGCATGGT
AAGCCATTACCACTTGATCCCTATGAACCGCGTAAAGTTGCCGAAACAGTGCAAGATATG
AAATTAAAATACGTGGTAATTACGTCGGTCGATCGTGATGATTTGGCCGATCGGGGGGCA
GCACATTTTGCGGCTACAGTGCGAGAAATTAAAGCATTAAATCCTGAATGCAAAGTCGAG
ATTTTAGTCCCGGATTTTCGTGGTCGAGTAGAACAAGCGGTTGCTATTTTGAAACAAAAT
CCACCCGATGTATTTAATCATAATTTAGAAAATGTGCCACGTTTATATCGCGAAATTCGC
CCGGGAGCGGATTATAAATGGTCATTAGAATTGCTTAAAATTTTCAAACAAGAGTTCCCT
AATATTCCCACTAAATCAGGCTTAATGGTTGGATTGGGCGAAACAAACGAAGAAATTTTA
GCGGTTATGCAAGATTTACGTGATCATGGTGTGACGATGTTGACCGTTGGTCAATATTTA
CAACCAAGCCGTCATCACTTAAGAGTAGAACGTTATGTTCCGCCTGCTGAATTTGAAATG
TTTCGCAGTGAAGCTGAAAAAATGGGATTTGAACACGCTGCTTGCGGTCCATTTGTTCGT
TCTTCTTATCATGCAGATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC
GACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

TNSAKIDSIKYGMRRHGLHSVCEEASCPNLHECFNHGTATFMIMGAICTRRCPFCDVAHG
KPLPLDPYEPRKVAETVQDMKLKYVVITSVDRDDLADRGAAHFAATVREIKALNPECKVE
ILVPDFRGRVEQAVAILKQNPPDVFNHNLENVPRLYREIRPGADYKWSLELLKIFKQEFP
NIPTKSGLMVGLGETNEEILAVMQDLRDHGVTMLTVGQYLQPSRHHLRVERYVPPAEFEM
FRSEAEKMGFEHAACGPFVRSSYHAD*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t7c,c8g,S3R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC62970.3
Recommended expression in:
Not Applicable