pssA-2 (P. syringae )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  pssA-2
Gene Name:  CDP-diacylglycerol-serine O-phosphatidyltransferase
Original Clone ID:
PsR175-21.1
Keyword:
None
Species:
Pseudomonas syringae
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
PsCD00423296
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1341nts
Open reading frame: 51 to 1391

GGGGGGGAATTCCTCAGAATATTTTGTTTACTTTAGAAGGAGATATACATATGCAGTCCA
CTTTCCGACGCTCGACCCTGGCAGCGCTTCGCGGTTTTGCCTTGCCGAGTGATGCAATCT
CCATTGTTCCTTCTGCCGCCGGCTGCCGGCGCTGCCTGCTGGAGAAGATCGCTTCGGCCA
CGCGGCGCATCTATATCATCGCGTTGTACCTGCAACAGGATGAAGCCGGCCAGGAAATCC
TCGACGCGCTGTACGCCGCCAAGGCTGCACGGCCCGAGCTGGACGTGGTGGTGCTGGTCG
ACTGGTTTCGCGCCCAGCGCGGCCTGATCGGTGCCGGTCGGCAACCGGGCAACTCGACCT
GGTATCAGGCACAGAACCTTGAATACGACGAAGAAGTGCCGATCTACGGCGTGCCGGTGC
AGACCCGCGAGCTGTTCGGCGTGCTGCACCTCAAGGGCAGCGTGATCGATGACTGCGTGA
TCTACAGCGGCGCGAGCATCAACAACGTCTACCTGCACAAGCTCGACAAATACCGGCCGG
ACCGTTATCACCTGATCGAAAGCGCGCCGCTGGCCGACTCTTTCGAGCACCTGGTGCAGA
GGGAAATACTGCCATCAGCGGCCGTGCACCGCCTGGACCTGCAATCGCCACCGAGCTCTC
GCAGCCTGCGCAGCGAAATCCGCGCCTTTCGCAGTGATTTGAAGCGCGCCACGTACGACA
CGTCGGCAGGCGACAAGGAAAACGGCCAGTTGCGGGTGATTCCGCTGCTGGGTGTGGGGC
CGCGCAACAGCCTCAACCGCGCGATCTGCGACCTGATCGCCTCCAGCAAGATCCAGCTGA
CCATCTGCACACCGTATTTCAACCTGCCCGTAGCGGTTACCCGCGAGGTCAATCGGGCGC
TCAAGCGCGGCGTAAAGGTCGATATCATCATTGGCGACAAGACCGCCAACGATTTCTTCA
TTGACCCGGACGAGCCGTTCAAAGTGATTTCGGCCCTGCCCTATCTGTACGAAATCAGCC
TGCGACACTTTGCCCACAAGCACCAGAAGGCCATCGCCCAGCAGCGTCTGAACGTGCACT
TGTGGAAGCACGGCGACAACACCTTCCACCTCAAGGGGATCTGGGTCGATCGGCGTTATA
CCTTGCTGACCGGCAACAACCTGAACCCTCGGGCGTTCAACCTCGACCTGGAAAACGGCT
TGCTGATCGATGATCCGCAAGGCCAGTGGACCGACCCGCGTGAAGCGGAAATCGCCCAAT
TGATGCGCAACACACAACGCGTCAACCAGTACGACGAGCTCGACACGCTGGCCAATTATC
CTGAAGGAGTGCGCAAGTTCCTCAAGCGGGTCAACCGGGTCAGGGTCGAGCGTTTGCTCT
ACCGCATTCTC

Protein sequence predicted based on nucleotide sequence

MQSTFRRSTLAALRGFALPSDAISIVPSAAGCRRCLLEKIASATRRIYIIALYLQQDEAG
QEILDALYAAKAARPELDVVVLVDWFRAQRGLIGAGRQPGNSTWYQAQNLEYDEEVPIYG
VPVQTRELFGVLHLKGSVIDDCVIYSGASINNVYLHKLDKYRPDRYHLIESAPLADSFEH
LVQREILPSAAVHRLDLQSPPSSRSLRSEIRAFRSDLKRATYDTSAGDKENGQLRVIPLL
GVGPRNSLNRAICDLIASSKIQLTICTPYFNLPVAVTREVNRALKRGVKVDIIIGDKTAN
DFFIDPDEPFKVISALPYLYEISLRHFAHKHQKAIAQQRLNVHLWKHGDNTFHLKGIWVD
RRYTLLTGNNLNPRAFNLDLENGLLIDDPQGQWTDPREAEIAQLMRNTQRVNQYDELDTL
ANYPEGVRKFLKRVNRVRVERLLYRIL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a92g,D31G;a95g,Y32C;
t488c,L163P;c550a,R184R;
g977a,R326H;a1082g,Q361R;
g1304a,S435N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in PsR175
Recommended expression in:
Not Applicable