rimO (T. thermophilus )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  rimO
Gene Name:  ribosomal protein S12 methylthiotransferase
Original Clone ID:
UR26-21.2
Keyword:
None
Species:
Thermus thermophilus
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
TtCD00423315
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1302nts
Open reading frame: 38 to 1339

TCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGGCGAAGATCGGTTTTGTCAG
CTTGGGCTGCCCCAAAGCCCTGGTGGACTCGGAGCAGATCCTTTCCCGGCTCAAGGCCCT
GGGGTACGAGACGAGCCCCAGCTACGAGGAGGCGGAGCTCGTCATCGTCAACACCTGCGG
CTTCATCAACGAGGCGGTGGAGGAGAGCCTCGAGGCCATCGGCGAGGCCCTCAAGGAAAA
CGGCAAGGTGGTGGTCACGGGCTGCCTGGGGGCGCGCCCCGAGAAGATCCGGGAGCGCCA
CCCCCAGGTGCTCGCCGTCACGGGTCCGGGGGAGGTGGAAAGGGTTCTGGAGGCGGTGCA
GGAGGTGCTTCCCGCCCCCAGGGACCCCTTCTTGGACCTCATCCCTCCCCAGGTCAAGCT
CACCCCGAGGCACTACGCCTACGTGAAGCTCTCCGAGGGGTGCGACCACCGGTGCAGCTT
CCGCATCATCCCTAAGCTGCGGGGAAGGCTCCGCTCCCGGGACGCCGCCGACGTGCTCGC
CGAGGCGGCGAGGCTCGTGGCCACGGGGACGAAGGAGCTCCTCCTCGTGGCCCAGGACCT
CTCGGCCTACGGGGTGGACCTGGGGCACCGGGAGAGCCTCTGGGGGGACCGGCCCGTGCG
GGCGGAGCTTAAGGACCTCCTCACCCACATGGCCGAGCTCGGCGTCTGGATCCGCCTCCA
CTACGTCTACCCCTACCCCCATGTCAAGGACCTCCTCCCCCTCATGGCCGAGGGGAAGGT
CCTGCCCTACCTGGACGTTCCCCTCCAGCACGCCTCCCCCCGGATCCTCCGCCTCATGCG
CCGCCCCGGGGGGTACGAGAGCCACCTCAAGACCCTCAAGGCCTGGCGGGAGGTGGTGCC
CGAGCTCGCCCTCCGCTCCACCTTCATCGTGGGCTTCCCCGGGGAGACGGAGGAGGACTT
CCAGATCCTCCTGGACTTCCTGGAGGAGGCGGAGCTGGACCGGGTGGGGGTCTTCGCCTA
CTCCCCGGTGGAGGGGGCGGAGGCGAACCGCCTTCCGGACCCCGTGCCCGAGGAGGTGAA
GGAGGAGCGCAAGGCGAGGCTTTTGGAGGTGCAGGCCCGGGTGAGCCTCAGGAAGAACCA
GCGTTTCGTGGGCAAGACCCTGGAGGTCCTGGTGGACGAGCTGCCCGAGCCCGGCCTCGC
CGTAGGCCGGACCTACCGCGACGCCCCCGGCGTGGACGGGGTGGTCTACGTGGAGACGGA
CGGCACCGTGCGGGTAGGGGAGAGGATCCCCGTGCGCATCCTTCGGGCGGACACCTACGA
CCTCCACGGGGTCCAGGCG

Protein sequence predicted based on nucleotide sequence

MAKIGFVSLGCPKALVDSEQILSRLKALGYETSPSYEEAELVIVNTCGFINEAVEESLEA
IGEALKENGKVVVTGCLGARPEKIRERHPQVLAVTGPGEVERVLEAVQEVLPAPRDPFLD
LIPPQVKLTPRHYAYVKLSEGCDHRCSFRIIPKLRGRLRSRDAADVLAEAARLVATGTKE
LLLVAQDLSAYGVDLGHRESLWGDRPVRAELKDLLTHMAELGVWIRLHYVYPYPHVKDLL
PLMAEGKVLPYLDVPLQHASPRILRLMRRPGGYESHLKTLKAWREVVPELALRSTFIVGF
PGETEEDFQILLDFLEEAELDRVGVFAYSPVEGAEANRLPDPVPEEVKEERKARLLEVQA
RVSLRKNQRFVGKTLEVLVDELPEPGLAVGRTYRDAPGVDGVVYVETDGTVRVGERIPVR
ILRADTYDLHGVQA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t445c,C149R
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in UR26
Recommended expression in:
Not Applicable