BCE_0427 (B. cereus ATCC 10987 )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BCE_0427
Gene Name:  sensor histidine kinase, putative
Original Clone ID:
APC67254.1_SG321_pMCSG7
Keyword:
partial cds
Species:
Bacillus cereus ATCC 10987
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
High number of silent and/or missense mutations in CDS.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Shonda Clancy
PSI
Midwest Center for Structural Genomics
BhCD00423393
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 546nts
Open reading frame: 51 to 596

CTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCAGATATTCTA
GGCTCGCAAATATAACAAAAATAATAAATACGAAATTAGAACTACGTGACGTATTGCAGC
GTGTGACAATGGCGATATCAGAGGAGATTGTTAGGTGCGACGCTGTTGGAATTTATTTAC
CCCAGGAAGATGGAACATTTAGAGGGTTTGCAGGAAAACCAGAGACTATAAATGGTGTAA
CGCTCGATACTCAAGTAATTAATCCTGAAATAGACTTACTGGCAAAAGAAGTTATTGAAA
CGAAAAGAACCATCTATATTCCTGATACCTCAAAGGATCATCGACCAGATCCGAGACCGG
TTAATGCATTCAAAATTAAGTCCTTATTAGCTCTGCCTATCTCATTTGGGCAAGAGTTAT
TTGGTCTGGTTTTTTTATTTGATTATGGAATTCCGATGAACTTAACAGATTCAGAAATTC
AAAGTGTTGAAGCTTATGTAAATATGGCTGCGGTTGCAATTCAAAACGCAAATAATTTAA
AACAAAAGGAAAACCTTATTGCTGAGAAGCAGCTGTTACTAAATGTTACCCGTTAACATT
GGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

RYSRLANITKIINTKLELRDVLQRVTMAISEEIVRCDAVGIYLPQEDGTFRGFAGKPETI
NGVTLDTQVINPEIDLLAKEVIETKRTIYIPDTSKDHRPDPRPVNAFKIKSLLALPISFG
QELFGLVFLFDYGIPMNLTDSEIQSVEAYVNMAAVAIQNANNLKQKENLIAEKQLLLNVT
R*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a42g,T14T;a60c,E20D;
a75g,V25V;t108c,C36C;
t132c,P44P;g133c,a135g,E45Q;
g162a,A54A;c177t,T59T;
c186t,G62G;g201t,T67T;
g204a,Q68Q;g211a,D71N;
g218a,G73E;t252g,T84T;
a257g,K86R;g261c,T87T;
t289c,Y97H;g297a,P99P;
a309g,P103P;c312t,V104V;
g313a,D105N;g318a,A106A;
t333c,S111S;t378g,L126L;
g400a,V134I;a405g,P135P;
g459t,A153A;c465t,V155V;
c468a,A156A;a483t,K161N;
c491a,T164K
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC67254.1
Recommended expression in:
Not Applicable