BCE_0427 (B. cereus ATCC 10987 )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BCE_0427
Gene Name:  sensor histidine kinase, putative
Original Clone ID:
APC67254.2_SG321_pMCSG7
Keyword:
partial cds
Species:
Bacillus cereus ATCC 10987
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
High number of silent and/or missense mutations in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Shonda Clancy
PSI
Midwest Center for Structural Genomics
BhCD00423394
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 564nts
Open reading frame: 51 to 614

CTTCTGGTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTTAAGTG
ATCAGACGAGATATTCTAGGCTCGCGAATATAACAAAAATAATAAATACGAAATTAGAAC
TACGTGACGTATTGCAGCGTGTGACAATGGCGATATCAGAGGAGATTGTTAGGTGCGACG
CTGTTGGAATTTATTTACCCCAGGAAGATGGAACATTTAGAGGGTTTGCAGGAAAACCAG
AGACTATAAATGGTGTAACGCTCGATACTCAAGTAATTAATCCTGAAATAGACTTACTGG
CAAAAGAAGTTATTGAAACGAAAAGAACCATCTATATTCCTGATACCTCAAAGGATCATC
GACCAGATCCGAGACCGGTTAATGCATTCAAAATTAAGTCCTTATTAGCTCTGCCTATCT
CATTTGGGCAAGAGTTATTTGGTCTGGTTTTTTTATTTGATTATGGAATTCCGATGAACT
TAACAGATTCAGAAATTCAAAGTGTTGAAGCTTATGTAAATATGGCTGCGGTTGCAATTC
AAAACGCAAATAATTTAAAACAAAAGGAAAACCTTATTGCTGAGAAGCAGCTGTTACTAA
ATGTTACCCGTTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGC
TTGCG

Protein sequence predicted based on nucleotide sequence

MLSDQTRYSRLANITKIINTKLELRDVLQRVTMAISEEIVRCDAVGIYLPQEDGTFRGFA
GKPETINGVTLDTQVINPEIDLLAKEVIETKRTIYIPDTSKDHRPDPRPVNAFKIKSLLA
LPISFGQELFGLVFLFDYGIPMNLTDSEIQSVEAYVNMAAVAIQNANNLKQKENLIAEKQ
LLLNVTR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a36g,A12A;a60g,T20T;
a78c,E26D;a93g,V31V;
t126c,C42C;t150c,P50P;
g151c,a153g,E51Q;g180a,A60A;
c195t,T65T;c204t,G68G;
g219t,T73T;g222a,Q74Q;
g229a,D77N;g236a,G79E;
t270g,T90T;a275g,K92R;
g279c,T93T;t307c,Y103H;
g315a,P105P;a327g,P109P;
c330t,V110V;g331a,D111N;
g336a,A112A;t351c,S117S;
t396g,L132L;g418a,V140I;
a423g,P141P;g477t,A159A;
c483t,V161V;c486a,A162A;
a501t,K167N;c509a,T170K
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC67254.2
Recommended expression in:
Not Applicable