MJ_1447 (M. jannaschii )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MJ_1447
Gene Name:  bifunctional formaldehyde-activating enzyme/3-hexulose-6-phosphate synthase
Original Clone ID:
32710
Keyword:
full-length cds
Species:
Methanocaldococcus jannaschii
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
Low quality silent and/or missense mutations found in CDS.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
MjCD00423536
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1146nts
Open reading frame: 51 to 1196

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGATAAAAT
TTGGAAAAGCAGTTTTGGGAAATGAAATTAATGCAATAGTTAATGTTGCATTAGGGAAAG
GAGAGTTAATTGAGAATGCCTTTACAAATGCTTTAACAAGAGGAAATTGTGTTTTTGCCG
ATTTAAGACCTGATTTAATAGTTAAACCATTAACCTTAGTTGTTCCAAGGCATAATATAG
AGAGTGAGATACAGGATGAGCTATTTCAGGGAGTTATTCAGTATGCAGTTGCCAAGGCAG
TTGCTGATTTAGATTTAGATGAAGATTTAAAGGTTGTTGTCTCTGTTAATGTCCCAGAGG
TTCCAATAACCAATTTAAATAAAAGAAAACTCTTCCAATACTTCTATGCCTCAGCAAAGT
TAGCTATAAACAGAGCTTTAAATGAATATCCTTCAAAAGAGAAGGTAAAGAAAGAGAAAT
ATAGAGCTTTGCATCCATTAGTTGGATTTAGGGATGTTAGATTGGAGTATCCTCCATATC
TACAAATTGCTTTGGATGTCCCAACTATGGAGAATTTGGAATTTTTGTTACAAACAATTC
CAAATAGCGACCACATCATCTTAGAGGCTGGAACACCACTAATTAAAAAGTTTGGTTTAG
AGGTTATTGAAATAATGAGAGAATATTTTGATGGCTTTATTGTTGCTGATTTAAAAACCT
TAGACACTGGAAGGGTTGAGGTAAGATTGGCATTTGAAGCAACAGCTAATGCAGTGGCAA
TAAGTGGAGTAGCACCAAAATCAACAATAATTAAAGCTATCCACGAATGTCAAAAATGTG
GTTTAATCAGCTATTTGGATATGATGAACGTCTCTGAACCTCAAAAATTATATGATTCAT
TAAAATTAAAGCCAGATGTTGTTATCTTGCATAGAGGGATTGATGAGGAGACATTTGGAA
TTAAAAAGGAATGGAAATTTAAGGAAAACTGCTTATTAGCAATTGCTGGAGGAGTTGGTG
TGGAGAATGTTGAAGAGCTTTTAAAAGAATATCAAATATTAATCGTTGGTAGAGCAATTA
CAAAATCAAAAGACCCAGGAAGAGTAATTAGGATGTTTATAAACAAGATGGGTTATGATA
TAGACACTTATAGGCTTTATTTTGATGAGGATGAGGATATAGGGGAGGAATTATAACGCG
ACTTAATTAATTTGGCGGATGAGAGAAGATTTTCAGCCTGATA

Protein sequence predicted based on nucleotide sequence

MIKFGKAVLGNEINAIVNVALGKGELIENAFTNALTRGNCVFADLRPDLIVKPLTLVVPR
HNIESEIQDELFQGVIQYAVAKAVADLDLDEDLKVVVSVNVPEVPITNLNKRKLFQYFYA
SAKLAINRALNEYPSKEKVKKEKYRALHPLVGFRDVRLEYPPYLQIALDVPTMENLEFLL
QTIPNSDHIILEAGTPLIKKFGLEVIEIMREYFDGFIVADLKTLDTGRVEVRLAFEATAN
AVAISGVAPKSTIIKAIHECQKCGLISYLDMMNVSEPQKLYDSLKLKPDVVILHRGIDEE
TFGIKKEWKFKENCLLAIAGGVGVENVEELLKEYQILIVGRAITKSKDPGRVIRMFINKM
GYDIDTYRLYFDEDEDIGEEL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g16a,E6K;g42t,K14N;
a88g,T30A;a130g,N44D;
a142g,N48D
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 375567
Recommended expression in:
Not Applicable