Gene:
Gene Symbol:
AF1970
Gene Name:
hypothetical protein
Original Clone ID:
799a2TNt7b1
Keyword:
full-length cds
Species:
Archaeoglobus fulgidus
Type:
cDNA
Vector Name:
pSB2
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
AsCD00423544
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 951nts
Open reading frame: 18 to 968
GTGGTCATATGTCCCTTGAAATTGTACCCCAGGAAGAAAGGCTGATTCGGGAAGGAAAAA
TTCTTGATGCTTTTGAGCTTGTAAAGCAATCCGCACCTGAAGAGCTAAGGGAGAGGATAG
AGAAGTCCTTCAGAGATGCTGAGGAGATTAAAAAGCTTGATGGAATCTGGAAAAAACTCA
TGCTGATACTTCACTTCGTTTACGCTTCTCAGGACGCGGTCGAGAGGCAGGACAAGCAAT
CTCTCGTTTCCTGCGTTGTTTCATCTGTTAATGCGATAAAGCTCACCATTGAGCTTGGAA
TGAAATCCATAACGCCGGTTTTGATGAGAAACGCTGCGAGAGCATTGATTCTGATGGACA
TGAAAGAAAACGCGGAGAGGATGTACACGGAGGCGGAAAAAATATGCGAGGAAAACAGAG
ATGAAGAGATGCTTGCAGCCATTGAAAACGACCTCGCAACGCTTTACTACGACATGGGAA
AGTACAACGAGGCCAAGGTTAAGGCCGAAGAGGCTTTAGAGATAAGGAGAAAGATTGGCA
CCAAAGAAGAGCTTGCGGAAAGCCTTGCGACCGCATCGGAGATTTACGTAAAACTCGGAG
ACTTTGACGAGGCTGAGGAGTGCTACAGAGAGGCGGAGAAGCTGTACAGGGAACTCGTGG
AGGAGGCAGAGTCCTTCAAGCTTAACCTGGCCATTCTGCTGAGCAACTACGCGATGTTCC
GAAAGAAGCTCGGAAGGTATCAGGAGGCTGAGAAAATGTTCAAGGAGGCGCTGGAGATTT
TCCAGAATCTCGAAAGGATTGATGCGGACTTCATGCAGTTCGTAGCCGCAGCGTACAGGC
ATCTTGGAGACCTCTACAGAGAAATGAAGGAGTACTCGAAGGCTGAGGAGTACTACAAGC
TTTCGAGGGAGAAGTTCAGGGACATACAGAAAAGGTGGGAAAGCGTAGCGAGCGAAGGGG
GATCCTAAACTGCAGAGATCCGAATTCGAGCTCCGTCG
Protein sequence predicted based on nucleotide sequence
EIVPQEERLIREGKILDAFELVKQSAPEELRERIEKSFRDAEEIKKLDGIWKKLMLILHF VYASQDAVERQDKQSLVSCVVSSVNAIKLTIELGMKSITPVLMRNAARALILMDMKENAE RMYTEAEKICEENRDEEMLAAIENDLATLYYDMGKYNEAKVKAEEALEIRRKIGTKEELA ESLATASEIYVKLGDFDEAEECYREAEKLYRELVEEAESFKLNLAILLSNYAMFRKKLGR YQEAEKMFKEALEIFQNLERIDADFMQFVAAAYRHLGDLYREMKEYSKAEEYYKLSREKF RDIQKRWESVASEGGS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a10c,a11c,a12c,K4P;g13c,E5Q;
t17a,t18a,V6E
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-799a
Recommended expression in:
Not Applicable
Vector Name:
pSB2
Synonyms:
pSB-2, pSB2_TN
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds N-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
TOPO modified pSB2 vector -by Invitrogen. Stop codon generated by adding an extra G to the reverse primer which puts the insert in-frame with vector-encoded stop when cloned into the TA site.
Size (bp):
5338
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
TOPO recognition site
TOPO
TOPO cloning site
5110
5119
bacterial origin
ori
ColE1 bacterial origin of replication
1420
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4984
5003
repressor protein gene
LacI
LacI cds
3770
4594
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5079
5096
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5267
5313
