44449762 (E. genome shotgun sequencing of the Sargasso Sea )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
9253d1BCt6p1
Keyword:
full-length cds
Species:
Environmental genome shotgun sequencing of the Sargasso Sea
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
EgCD00423584
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1227nts
Open reading frame: 51 to 1277

TCCCTCAGAAAATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTCCGATAATTA
CTGCGTTACGCACGATCCGTTTACCAGAACGTCTTAAATTGATATGGGTGGAGGTTGAAA
CTGAGGATGGCCTTACAGGTCTCGGGGAAACCTTTCGCGGAGCCCAGGCAGTCGAAGCTG
TACTGCATGAGCAAACGGCGCCCGCCATTATCGGCCGGGCAGCTGAAAATATTACTAGCA
TCTCTAGTGAGCTGTTAAACCCGTATGTGGGTTTCGGGTCATCCTCGGCTGAAGTTAGAG
CAGCCAGCGCTGTCGACATAGCGTTGTGGGATCTTGCTGGCCAGCGAGCAGGCGTACCAC
TGCACGTGGCTCTCGGTGGCGCGGCCCGCGATCGGGTTCCTGTATACGCAACATGCGCGG
GTTATGACTTTAATACCTCTCTGGGGGGACGTCGCAGTATTGGCAGTGCAGAGTTATCCA
CGGGTCCGTACGACGATCAAGTCGCCTTCATGAGAGATGCAGGCGTGCTCGCTGAGTCGT
TGGTTGCTGAAGGTTATGCCGCAATGAAGATCTGGCCCTTTGACGATTTCGCGAGCATTA
CCCCGCATCACATATCTCTTACTGACCTGAAAGATGGGCTCGAGCCATTTCGAAAAATCC
GTGCTGCAGTGGGACAGCGCATTGAAATCATGTGTGAATTACATAGTCTGTGGGGCACAC
ACGCAGCGGCCCGGATTTGCAACGCATTGGCGGACTACGGTGTCCTTTGGGTTGAGGACC
CTATAGCTAAGATGGATAATATCCCGGCCGTAGCCGATCTGCGTCGCCAGACGCGTGCTC
CCATTTGTGGGGGCGAAAACTTAGCCGGTACCCGCAGATTCCATGAGATGCTGTGCGCCG
ATGCGATAGACTTTGTGATGCTCGATCTGACTTGGTGTGGAGGCTTGTCAGAAGGTCGAA
AAATCGCTGCATTAGCCGAAACACACGCGCGGCCGCTGGCTCCACATGATTGCACCGGGC
CTGTCGCATTAATGGCCGGCCTGCACCTCGCGTTGCATGCTCCGACGGCAATTTTCCAAG
AGGTTGTGCGTGCCTCCCTGGCGACCTGGTATGCTGACTTAGTAGATCACTTACCAGTCA
TTCAGGAAGGTATCGCACTGGCCCCGACTCGCCCCGGACTGGGCACCGCTTTATTGCCTC
ATGTTCGTAAAATTGCTGGTGCAGTGGTTAGAGAATCTGGGAAGCCGCGGGAAGGGCATC
ATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

PIITALRTIRLPERLKLIWVEVETEDGLTGLGETFRGAQAVEAVLHEQTAPAIIGRAAEN
ITSISSELLNPYVGFGSSSAEVRAASAVDIALWDLAGQRAGVPLHVALGGAARDRVPVYA
TCAGYDFNTSLGGRRSIGSAELSTGPYDDQVAFMRDAGVLAESLVAEGYAAMKIWPFDDF
ASITPHHISLTDLKDGLEPFRKIRAAVGQRIEIMCELHSLWGTHAAARICNALADYGVLW
VEDPIAKMDNIPAVADLRRQTRAPICGGENLAGTRRFHEMLCADAIDFVMLDLTWCGGLS
EGRKIAALAETHARPLAPHDCTGPVALMAGLHLALHAPTAIFQEVVRASLATWYADLVDH
LPVIQEGIALAPTRPGLGTALLPHVRKIAGAVVRESGKPREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g5t,R2I;c44t,P15L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9253d
Recommended expression in:
Not Applicable