ytsJ (B. subtilis )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  ytsJ
Gene Name:  NAD-dependent malic enzyme 4
Original Clone ID:
6398b1BCt1b1
Keyword:
None
Species:
Bacillus subtilis
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BsCD00423727
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1254nts
Open reading frame: 51 to 1304

TTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTTCATTAAGAG
AAGAAGCATTACACCTGCATAAAGTCAACCAGGGGAAACTGGAGTCTAAATCAAAAGTAG
AGGTCAGAAATGCGAAAGATTTAAGCCTCGCGTATTCTCCGGGTGTTGCAGAACCGTGTA
AGGATATTCATGAAGACATTAACAAAGTATATGATTATACAATGAAGGGGAACATGGTAG
CAGTTGTAACTGACGGTACAGCTGTGCTTGGCCTTGGAAACATCGGCCCTGAAGCGGCGC
TTCCTGTTATGGAAGGGAAAGCAGTTCTTTTCAAAAGCTTTGCGGGCGTAGATGCGTTCC
CGATTGCTTTAAACACAAACGATGTTGATAAAATCGTTGAAACTGTAAAATTGCTGGAAC
CGACATTTGGCGGCGTCAACCTTGAAGATATTGCAGCGCCAAACTGCTTTATCATTGAAG
AGCGCCTCAAAAAAGAAACAAACATCCCGGTTTTCCATGATGATCAGCACGGTACTGCCA
TTGTAACCGTAGCAGGTCTTGTGAACGCGCTGAAACTGTCTGGAAAATCCATGTCATCCA
TTAAAGTTGTCGCAAACGGCGCGGGTGCGGCTGGCATTGCGATTATCAAGCTTCTTCATC
ATTATGGCGTTCGCGACATTGTGATGTGCGATTCCAAAGGGGCCATTTATGAAGGGCGCC
CGAACGGCATGAACGATGTCAAAAATGAAGTGGCGAAGTTCACGAACCAAGATCGCAAAG
ACGGTTCTCTGAAAGATGTTATCGTTGATGCTGACGTATTTATCGGTGTCTCTGTAGCAG
GCGCGTTAACAAAAGAAATGGTTCAAAGCATGGCAAAAGATCCGATTATCTTTGCAATGG
CAAATCCAAATCCGGAAATCATGCCGGAAGATGCGCGTGAAGCCGGAGCAAGCGTTGTCG
GAACTGGCCGTTCTGACTTCCCGAACCAAGTAAACAATGTTCTTGCATTCCCTGGTATTT
TCCGCGGAGCGCTTGATGTGCGTGCCACTCATATTAACGAGGAAATGAAAATCGCAGCGG
TTGAAGCCATTGCTTCACTGGTTTCAGAAGATGAGCTTAGCGCAGACTATGTCATCCCTG
CACCGTTTGATAAACGTGTTGCGCCTGCTGTTGCGAAGGCTGTGGCAAAAGCTGCGATGG
AAACAGGTGTTGCAAGAATCACTGTTGACCCTGAGGAAGTCGCAGAAAAAACAAGAAAAC
TTACGATTATTGGTAAAGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCC
G

Protein sequence predicted based on nucleotide sequence

SLREEALHLHKVNQGKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMKG
NMVAVVTDGTAVLGLGNIGPEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETVK
LLEPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGKS
MSSIKVVANGAGAAGIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTNQ
DRKDGSLKDVIVDADVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAGA
SVVGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSADY
VIPAPFDKRVAPAVAKAVAKAAMETGVARITVDPEEVAEKTRKLTIIGKEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g1225a,E409K
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-6398b
Recommended expression in:
Not Applicable